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Review

Aspects of In Vitro Plant Tissue Culture and Breeding of Asparagus: A Review

1
Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora, CSIC-UMA, Algarrobo-Costa, 29750 Málaga, Spain
2
Department of Biology and Geology, Agri-Food Campus of International Excellence (CeiA3) and Research Center CIAMBITAL, University of Almería, 04120 Almería, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Othmane Merah
Horticulturae 2022, 8(5), 439; https://doi.org/10.3390/horticulturae8050439
Received: 7 April 2022 / Revised: 11 May 2022 / Accepted: 12 May 2022 / Published: 14 May 2022
(This article belongs to the Special Issue Advances in Asparagus Production, Genomics and Breeding)
In vitro plant tissue culture and biotechnology used to assist and support the development of plant breeding when classical methods of propagation must be accelerated or it was necessary to overcome barriers inaccessible by classical approaches. In asparagus, to improve multiple breeding tasks, a high number of in vitro methods have been used, such as plant regeneration methods through organogenesis, embryogenesis, manipulation of ploidy, protoplast isolation, genetic manipulation (protoplast fusion, genetic transformation), embryo rescue and germplasm preservation (in vitro, in vitro slow growth, cryopreservation). Plant tissue culture methods can overcome multiple problems in asparagus breeding such as, barriers of self and cross-incompatibility between asparagus species through embryo rescue of interspecific hybrids and protoplast fusion or genetic transformation, introgression of new genes, clonal propagation of elite genotypes of asparagus, mass screening, and the generation of haploid and polyploid genotypes, among others, becoming the tool of choice for asparagus breeding programs. Some of these in vitro methods are still under development. View Full-Text
Keywords: electroporation; embryo rescue; genetic transformation; micropropagation; ploidy modifications; protoplasts electroporation; embryo rescue; genetic transformation; micropropagation; ploidy modifications; protoplasts
MDPI and ACS Style

Encina, C.L.; Regalado, J.J. Aspects of In Vitro Plant Tissue Culture and Breeding of Asparagus: A Review. Horticulturae 2022, 8, 439. https://doi.org/10.3390/horticulturae8050439

AMA Style

Encina CL, Regalado JJ. Aspects of In Vitro Plant Tissue Culture and Breeding of Asparagus: A Review. Horticulturae. 2022; 8(5):439. https://doi.org/10.3390/horticulturae8050439

Chicago/Turabian Style

Encina, Carlos L., and José J. Regalado. 2022. "Aspects of In Vitro Plant Tissue Culture and Breeding of Asparagus: A Review" Horticulturae 8, no. 5: 439. https://doi.org/10.3390/horticulturae8050439

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