Biological Control of Pear Valsa Canker Caused by Valsa pyri Using Penicillium citrinum
Abstract
:1. Introduction
2. Materials and Methods
2.1. Pathogenic Fungal Isolates
2.2. Isolation of Endophytic Fungi from the Branches of Pear
2.3. Dual Culture Test for Screening Potential Biocontrol Fungi
2.4. Assessment of the Impact of Antagonistic Strains on V. pyri Hyphal Morphology
2.5. Assessment of Antagonistic Strain-Derived Culture Filtrates on V. pyri Mycelial Growth
2.6. Assessment of Antagonistic Strain-Derived Culture Filtrates on V. pyti Conidial Germination
2.7. Disease Suppression Assay
2.8. Antagonistic Strain Identification
3. Results
3.1. Isolation and Assessment of Antifungal Activity of ZZ1 against Phytopathogenic Fungi
3.2. Assessment of the Antagonistic Effects of Isolate ZZ1 on V. pyri Hyphal Morphology
3.3. Analysis of the Ability of ZZ1 Conidial Suspensions to Control Pear Valsa Canker Disease
3.4. Assessment of the Ability of ZZ1 Culture Filtrates to Suppress V. pyri Mycelial Growth and Spore Germination
3.5. Assessment of the Ability of ZZ1 Culture Filtrates to Control Pear Valsa Canker Disease
3.6. Identification of Strain ZZ1
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Endophytic Strains | Inhibition (%) |
---|---|
ZZ1 | 95.2 ± 2.1 |
ZZ2 | 7.4 ± 0.8 |
ZZ3 | 3.9 ± 0.7 |
ZZ4 | 5.7 ± 1.1 |
ZZ5 | 2.7 ± 0.8 |
ZZ6 | 3.5 ± 1.2 |
ZZ7 | 12.3 ± 1.4 |
ZZ8 | 32.5 ± 3.1 |
ZZ9 | 13.5 ± 2.3 |
ZZ10 | 5.3 ± 1.6 |
ZZ11 | 3.5 ± 0.5 |
ZZ12 | 55.0 ± 2.6 |
ZZ13 | 22.3 ± 3.1 |
ZZ14 | 15.4 ± 2.4 |
ZZ15 | 5.9 ± 1.5 |
ZZ16 | 14.6 ± 2.4 |
ZZ17 | 58.7 ± 3.2 |
ZZ18 | 8.6 ± 0.9 |
ZZ19 | 5.4 ± 0.4 |
ZZ20 | 23.5 ± 3.5 |
ZZ21 | 16.5 ± 2.6 |
ZZ22 | 7.4 ± 1.8 |
ZZ23 | 8.9 ± 1.1 |
ZZ24 | 3.3 ± 0.8 |
ZZ25 | 5.4 ± 1.3 |
ZZ26 | 8.4 ± 2.1 |
ZZ27 | 4.9 ± 0.5 |
ZZ28 | 9.4 ± 1.7 |
ZZ29 | 10.5 ± 2.1 |
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Yuan, H.; Shi, B.; Huang, T.; Zhou, Z.; Wang, L.; Hou, H.; Tu, H. Biological Control of Pear Valsa Canker Caused by Valsa pyri Using Penicillium citrinum. Horticulturae 2021, 7, 198. https://doi.org/10.3390/horticulturae7070198
Yuan H, Shi B, Huang T, Zhou Z, Wang L, Hou H, Tu H. Biological Control of Pear Valsa Canker Caused by Valsa pyri Using Penicillium citrinum. Horticulturae. 2021; 7(7):198. https://doi.org/10.3390/horticulturae7070198
Chicago/Turabian StyleYuan, Hongbo, Bingke Shi, Tianxiang Huang, Zengqiang Zhou, Li Wang, Hui Hou, and Hongtao Tu. 2021. "Biological Control of Pear Valsa Canker Caused by Valsa pyri Using Penicillium citrinum" Horticulturae 7, no. 7: 198. https://doi.org/10.3390/horticulturae7070198