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Article

Identification of a Major QTL and Development of a CAPS Marker Associated with Stem Blight Resistance in BC1 Populations Derived from Interspecific Crosses Between Asparagus officinalis L. and Asparagus kiusianus Makino

by
Yanxing Lu
1,*,
Yoko Takeuchi
2,
Emika Kakizoe
1,
Eri Kato
1,
Masaru Matsumoto
3,
Yoshiyuki Yamagata
2,
Jun-ichiro Masuda
4,
Yuki Mizunoe
2,
Keita Tomiyoshi
5,
Kaori Sakai
2,
Mostafa Abdelrahman
6,
Akira Kanno
6,
Takao Ikeuchi
7,
Mitsutaka Mori
7,
Kyoko Murakami
7,
Atsuko Uragami
8,
Shin-ichi Watanabe
9 and
Yukio Ozaki
2,*
1
Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Motooka, Nishi-ku, Fukuoka 819-0395, Japan
2
Faculty of Agriculture, Kyushu University, Motooka, Nishi-ku, Fukuoka 819-0395, Japan
3
Institute of Tropical Agriculture, Kyushu University, Motooka, Nishi-ku, Fukuoka 819-0395, Japan
4
Faculty of Agriculture, Miyazaki University, Miyazaki 889-2192, Japan
5
Faculty of Agriculture, Kagawa University, Mikicho 761-0701, Kagawa, Japan
6
Graduate School of Life Science, Tohoku University, 2-1-1, Katahira, Aoba-ku, Sendai 980-8577, Japan
7
Kagawa Prefectural Agricultural Experiment Station, 1534-1 Ayagawa, Ayauta, Kagawa 761-2306, Japan
8
Institute of Vegetable and Floriculture Science, NARO (National Agriculture and Food Research Organization), Tsukuba 305-8519, Ibaraki, Japan
9
Kyushu Okinawa Agricultural Research Center, NARO (National Agriculture and Food Research Organization), 1823-1 Miimachi, Kurume 839-8503, Fukuoka, Japan
*
Authors to whom correspondence should be addressed.
Horticulturae 2026, 12(8), 953; https://doi.org/10.3390/horticulturae12080953
Submission received: 3 July 2026 / Revised: 26 July 2026 / Accepted: 28 July 2026 / Published: 2 August 2026
(This article belongs to the Section Biotic and Abiotic Stress)
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Abstract

Stem blight, caused by Phomopsis asparagi, is the most serious disease affecting the cultivation of asparagus (Asparagus officinalis L.) in the warm regions of East and Southeast Asia, including Japan. Although Asparagus kiusianus, a wild species endemic to Japan, shows strong resistance to this disease and is cross-compatible with cultivated asparagus, the genetic basis of this resistance remains unclear. In this study, we used BC1 populations from interspecific crosses between A. officinalis and A. kiusianus to dissect stem blight resistance inheritance and identify markers suitable for marker-assisted selection. Disease severity was evaluated using P. asparagi inoculation, and RAD sequencing was used to construct a linkage map and perform QTL analysis. Segregation patterns suggested that resistance was controlled by major genes and polygenic factors which depended on the cross combination. A major QTL was detected on chromosome 1 and SNP_PRK showed a significant association (LOD > 3). A dCAPS marker derived from SNP_PRK was associated with resistance, with approximately 80% of individuals carrying the associated genotype showing resistance. CAPS marker PR1 distinguished the same genotype classes and produced results consistent with those obtained using PRK in the evaluated materials. PR1 may therefore facilitate resistant individual selection in breeding programs.
Keywords: Asparagus officinalis; Asparagus kiusianus; stem blight; Phomopsis asparagi; disease resistance; RAD sequencing; SNP marker; dCAPS marker; QTL analysis; marker-assisted selection Asparagus officinalis; Asparagus kiusianus; stem blight; Phomopsis asparagi; disease resistance; RAD sequencing; SNP marker; dCAPS marker; QTL analysis; marker-assisted selection

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MDPI and ACS Style

Lu, Y.; Takeuchi, Y.; Kakizoe, E.; Kato, E.; Matsumoto, M.; Yamagata, Y.; Masuda, J.-i.; Mizunoe, Y.; Tomiyoshi, K.; Sakai, K.; et al. Identification of a Major QTL and Development of a CAPS Marker Associated with Stem Blight Resistance in BC1 Populations Derived from Interspecific Crosses Between Asparagus officinalis L. and Asparagus kiusianus Makino. Horticulturae 2026, 12, 953. https://doi.org/10.3390/horticulturae12080953

AMA Style

Lu Y, Takeuchi Y, Kakizoe E, Kato E, Matsumoto M, Yamagata Y, Masuda J-i, Mizunoe Y, Tomiyoshi K, Sakai K, et al. Identification of a Major QTL and Development of a CAPS Marker Associated with Stem Blight Resistance in BC1 Populations Derived from Interspecific Crosses Between Asparagus officinalis L. and Asparagus kiusianus Makino. Horticulturae. 2026; 12(8):953. https://doi.org/10.3390/horticulturae12080953

Chicago/Turabian Style

Lu, Yanxing, Yoko Takeuchi, Emika Kakizoe, Eri Kato, Masaru Matsumoto, Yoshiyuki Yamagata, Jun-ichiro Masuda, Yuki Mizunoe, Keita Tomiyoshi, Kaori Sakai, and et al. 2026. "Identification of a Major QTL and Development of a CAPS Marker Associated with Stem Blight Resistance in BC1 Populations Derived from Interspecific Crosses Between Asparagus officinalis L. and Asparagus kiusianus Makino" Horticulturae 12, no. 8: 953. https://doi.org/10.3390/horticulturae12080953

APA Style

Lu, Y., Takeuchi, Y., Kakizoe, E., Kato, E., Matsumoto, M., Yamagata, Y., Masuda, J.-i., Mizunoe, Y., Tomiyoshi, K., Sakai, K., Abdelrahman, M., Kanno, A., Ikeuchi, T., Mori, M., Murakami, K., Uragami, A., Watanabe, S.-i., & Ozaki, Y. (2026). Identification of a Major QTL and Development of a CAPS Marker Associated with Stem Blight Resistance in BC1 Populations Derived from Interspecific Crosses Between Asparagus officinalis L. and Asparagus kiusianus Makino. Horticulturae, 12(8), 953. https://doi.org/10.3390/horticulturae12080953

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