Production of Single-Cell Protein from Fruit Peel Wastes Using Palmyrah Toddy Yeast
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Chemicals
2.2. Physicochemical Properties of Fruit Peels
2.3. Culture Media and Inoculum Preparations
2.4. Production of SCP Using Liquid State Fermentation Process
2.5. Selection of the Best Substrate for SCP Production
2.6. Optimization of Fermentation Condition and Comparison with Control Medium
2.7. Effect of Nucleic Acid Reduction on SCP Production
2.8. Estimation of Amino Acid Content
2.9. Statistical Analysis
3. Results and Discussion
3.1. Compositional Analysis of Substrates
3.2. Selection of the Best Substrate for SCP Production
3.3. Optimization of Process Parameters in the SCP Production
3.3.1. Optimization of pH for SCP Production
3.3.2. Optimization of Incubation Temperature for SCP Production
3.3.3. Optimization of Incubation Time for the SCP Production
3.3.4. Effect of Optimization on Biomass and Protein Content
3.3.5. Effect of Nucleic Acid Reduction
3.3.6. Nutrient Analysis
4. Conclusions and Future Perspectives
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fruit Peel | Yield (%) | pH | TSS (%) | Reducing Sugar (%) | Moisture (%) | Ash (%) 1 | Fat (%) 1 | Protein (%) 1 | Total Carbohydrate (%) 1 |
---|---|---|---|---|---|---|---|---|---|
Pineapple | 15.3 ± 0.9 b | 3.7 ± 0.0 e | 10.8 ± 0.0 c | 2.6 ± 0.1 b | 84.7 ± 0.9 b | 4.5 ± 0.3 c | 0.9 ± 0.1 c | 6.9 ± 0.1 c,d | 87.7 ± 0.4 b |
Watermelon | 5.0 ± 0.4 c | 5.4 ± 0.0 b | 3.2 ± 0.0 f | 1.8 ± 0.1 c,d | 95.0 ± 0.4 a | 5.5 ± 0.2 b,c | 1.5 ± 0.1b | 10.3 ± 0.3 b | 82.6 ± 0.5 c |
Papaya | 8.4 ± 0.2 c | 5.5 ± 0.0 a | 6.5 ± 0.0 e | 5.8 ± 0.1 a | 91.6 ± 0.2 a | 6.4 ± 0.4 a,b | 1.1 ± 0.1 b,c | 11.3 ± 0.6 a | 81.2 ± 0.9 c |
Sour orange | 25.2 ± 2.5 a | 4.1 ± 0.0 d | 12.3 ± 0.0 b | 1.2 ± 0.1 d | 74.8 ± 2.5 c | 6.1 ± 1.0 a,b | 1.4 ± 0.1 b,c | 7.2 ± 0.1 c | 85.4 ± 0.9 a |
Banana | 26.1 ± 2.1 a | 4.8 ± 0.0 c | 7.1 ± 0.0 d | 3.1 ± 0.4 b | 73.9 ± 2.1 c | 7.4 ± 0.3 a | 2.6 ± 0.4 a | 6.4 ± 0.2 d | 83.6 ± 0.7 a |
Mango | 23.8 ± 0.2 a | 4.1 ± 0.0 d | 16.8 ± 0.0 a | 2.4 ± 0.3 b,c | 76.2 ± 0.2 c | 4.2 ± 0.5 c | 2.5 ± 0.3 a | 6.2 ± 0.2 d | 87.1 ± 0.8 a |
Fruit Peel | Dry Biomass (g/L) | Crude Protein Content (%) |
---|---|---|
Pineapple | 9.40 ± 0.53 a,b | 49.7 ± 1.3 b |
Watermelon | 5.33 ± 0.61 c | 45.2 ± 0.7 c |
Papaya | 11.73 ± 0.81 a | 52.4 ± 0.4 a |
Sour orange | 9.13 ± 0.64 a,b | 29.5 ± 1.2 d |
Banana | 7.77 ± 1.88 b,c | 30.4 ± 0.6 d |
Mango | 8.61 ± 0.90 b | 24.6 ± 0.2 e |
Condition | Dry Biomass (g/L) | Crude Protein Content (%) |
---|---|---|
Control medium | 27.75 ± 0.93 A | 54.3 ± 0.6 b |
Papaya peel medium | 23.15 ± 2.31 B | 56.1 ± 0.4 a |
Condition | Dry Biomass (g/L) | Crude Protein Content (%) |
---|---|---|
Before nucleic acid reduction treatment | 23.2 ± 2.3 A | 56.1 ± 0.4 a |
After nucleic acid reduction treatment | 16.7 ± 1.2 B | 45.3 ± 0.3 b |
Description | Amino Acid | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
His 1 | Ile 1 | Leu 1 | Lys 1 | Met + Cys 1,2 | Phe + Tyr 1,3 | Thr 1 | Trp 1 | Val 1 | Glu | Arg | |
SCP from papaya peel based medium | 8.5 | 14.6 | 29.2 | 8.2 | 14.4 | 6.5 | 5.5 | 9.2 | 14.7 | 12.6 | 22.5 |
Amino acid requirement (FAO/WHO/UNU, 2007 [39]) | 15 | 30 | 59 | 45 | 22 | 38 | 23 | 6 | 39 | NR | NR |
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Thiviya, P.; Gamage, A.; Kapilan, R.; Merah, O.; Madhujith, T. Production of Single-Cell Protein from Fruit Peel Wastes Using Palmyrah Toddy Yeast. Fermentation 2022, 8, 355. https://doi.org/10.3390/fermentation8080355
Thiviya P, Gamage A, Kapilan R, Merah O, Madhujith T. Production of Single-Cell Protein from Fruit Peel Wastes Using Palmyrah Toddy Yeast. Fermentation. 2022; 8(8):355. https://doi.org/10.3390/fermentation8080355
Chicago/Turabian StyleThiviya, Punniamoorthy, Ashoka Gamage, Ranganathan Kapilan, Othmane Merah, and Terrence Madhujith. 2022. "Production of Single-Cell Protein from Fruit Peel Wastes Using Palmyrah Toddy Yeast" Fermentation 8, no. 8: 355. https://doi.org/10.3390/fermentation8080355