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Article

Optimization of Synthetic Media Composition for Kluyveromyces marxianus Fed-Batch Cultivation

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Laboratory of Bioengineering, Latvian State Institute of Wood Chemistry, Dzerbenes Street 27, LV-1006 Riga, Latvia
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Institute of General Chemical Engineering, Faculty of Materials Science and Applied Chemistry, Riga Technical University, Paula Valdena Street 3, LV-1658 Riga, Latvia
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Biotehniskais Centrs AS, Dzerbenes Street 27, LV-1006 Riga, Latvia
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Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas Street 1, LV-1004 Riga, Latvia
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Author to whom correspondence should be addressed.
Academic Editor: Rosanna Tofalo
Fermentation 2021, 7(2), 62; https://doi.org/10.3390/fermentation7020062
Received: 25 March 2021 / Revised: 9 April 2021 / Accepted: 13 April 2021 / Published: 15 April 2021
The Kluyveromyces marxianus yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order to intensify the biosynthesis rate of a target product, reaching high biomass concentrations in the reaction medium is mandatory. Fed-batch processes are an attractive and efficient way how to achieve high cell densities. However, depending on the physiology of the particular microbial strain, an optimal media composition should be used to avoid by-product synthesis and, subsequently, a decrease in overall process effi-ciency. Thus, the aim of the present study was to optimise the synthetic growth medium and feeding solution compositions (in terms of carbon, nitrogen, phosphorous, magnesium, and calcium concentrations) for high cell density K. marxianus fed‑batch cultivations. Additionally, the biomass yields from the vitamin mixture and other macro/microelements were identified. A model predictive control algorithm was successfully applied for a fed-batch cultivation control. Biomass growth and substrate consumption kinetics were compared with the mathematical model predictions. Finally, 2‑phenylethanol biosynthesis was induced and its productivity was estimated. The determined optimal macronutrient ratio for K. marxianus biomass growth was identified as C:N:P = 1:0.07:0.011. The maximal attained yeast biomass concentration was close to 70 g·L-1 and the 2-PE biosynthesis rate was 0.372 g·L−1·h−1, with a yield of 74% from 2-phenylalanine. View Full-Text
Keywords: Kluyveromyces marxianus; 2-phenylethanol; fed-batch; cultivation; bioreactor; model predictive control (MPC) Kluyveromyces marxianus; 2-phenylethanol; fed-batch; cultivation; bioreactor; model predictive control (MPC)
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MDPI and ACS Style

Dubencovs, K.; Liepins, J.; Suleiko, A.; Suleiko, A.; Vangravs, R.; Kassaliete, J.; Scerbaka, R.; Grigs, O. Optimization of Synthetic Media Composition for Kluyveromyces marxianus Fed-Batch Cultivation. Fermentation 2021, 7, 62. https://doi.org/10.3390/fermentation7020062

AMA Style

Dubencovs K, Liepins J, Suleiko A, Suleiko A, Vangravs R, Kassaliete J, Scerbaka R, Grigs O. Optimization of Synthetic Media Composition for Kluyveromyces marxianus Fed-Batch Cultivation. Fermentation. 2021; 7(2):62. https://doi.org/10.3390/fermentation7020062

Chicago/Turabian Style

Dubencovs, Konstantins, Janis Liepins, Arturs Suleiko, Anastasija Suleiko, Reinis Vangravs, Jana Kassaliete, Rita Scerbaka, and Oskars Grigs. 2021. "Optimization of Synthetic Media Composition for Kluyveromyces marxianus Fed-Batch Cultivation" Fermentation 7, no. 2: 62. https://doi.org/10.3390/fermentation7020062

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