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Removal of Bacterial Contamination from Bioethanol Fermentation System Using Membrane Bioreactor

Swedish Centre for Resource Recovery, University of Borås, 501 90 Borås, Sweden
Flemish Institute for Technological Research, VITO NV, Boeretang 200, B-2400 Mol, Belgium
Department of Environmental Sciences and Engineering, Graduate School of Science, Engineering and Technology, Istanbul Technical University, Maslak, 34467 Sarıyer, Istanbul, Turkey
Author to whom correspondence should be addressed.
Fermentation 2018, 4(4), 88;
Received: 25 September 2018 / Revised: 12 October 2018 / Accepted: 16 October 2018 / Published: 18 October 2018
(This article belongs to the Special Issue Ethanol and Value-Added Co-Products)
A major issue hindering efficient industrial ethanol fermentation from sugar-based feedstock is excessive unwanted bacterial contamination. In industrial scale fermentation, reaching complete sterility is costly, laborious, and difficult to sustain in long-term operation. A physical selective separation of a co-culture of Saccharomyces cerevisiae and an Enterobacter cloacae complex from a buffer solution and fermentation media at dilution rates of 0.1–1 1/h were examined using an immersed membrane bioreactor (iMBR). The effect of the presence of yeast, inoculum size, membrane pore size, and surface area, backwashing and dilution rate on bacteria removal were assessed by evaluating changes in the filtration conditions, medium turbidity, and concentration of compounds and cell biomass. The results showed that using the iMBR with dilution rate of 0.5 1/h results in successful removal of 93% of contaminating bacteria in the single culture and nearly complete bacteria decontamination in yeast-bacteria co-culture. During continuous fermentation, application of lower permeate fluxes provided a stable filtration of the mixed culture with enhanced bacteria washout. This physical selective separation of bacteria from yeast can enhance final ethanol quality and yields, process profitability, yeast metabolic activity, and decrease downstream processing costs. View Full-Text
Keywords: membrane bioreactor; filtration; bacterial decontamination; fermentation membrane bioreactor; filtration; bacterial decontamination; fermentation
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MDPI and ACS Style

Mahboubi, A.; Cayli, B.; Bulkan, G.; Doyen, W.; De Wever, H.; Taherzadeh, M.J. Removal of Bacterial Contamination from Bioethanol Fermentation System Using Membrane Bioreactor. Fermentation 2018, 4, 88.

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