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Fermentation 2017, 3(3), 31;

Phenols Removal from Hemicelluloses Pre-Hydrolysate by Laccase to Improve Butanol Production

Centre National en Électrochimie et en Technologies Environnementales, Shawinigan G9N 6V8, QC, Canada
Department of Chemical Engineering, Polytechnique Montréal, Montréal H3T 1J4, QC, Canada
Department of Wood and Forest Sciences, Université Laval, 2405 rue de la Terrasse, Québec G1V 0A6, QC, Canada
Author to whom correspondence should be addressed.
Received: 9 May 2017 / Revised: 23 June 2017 / Accepted: 26 June 2017 / Published: 30 June 2017
(This article belongs to the Special Issue Biofuels and Biochemicals Production)
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Phenolic compounds are important inhibitors of the microorganisms used in the Acetone-Butanol-Ethanol (ABE) fermentation. The degradation of phenolic compounds in a wood pre-hydrolysate, a potential substrate for the production of ABE, was studied in this article. First, physicochemical methods for detoxification such as nanofiltration and flocculation were applied and the best combination was selected. With a flocculated sample, the concentration of phenolic compounds decreases from 1.20 to 0.28 g/L with the addition of a solid laccase at optimum conditions, which is below the phenolic compounds limit of inhibition. This results in an increase in butanol production, more than double, compared to a pre-hydrolysate non-treated with laccase enzymes. View Full-Text
Keywords: phenolic compounds; detoxification; butanol; pre-hydrolysate; laccase enzymes phenolic compounds; detoxification; butanol; pre-hydrolysate; laccase enzymes

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Allard-Massicotte, R.; Chadjaa, H.; Marinova, M. Phenols Removal from Hemicelluloses Pre-Hydrolysate by Laccase to Improve Butanol Production. Fermentation 2017, 3, 31.

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