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Fermentation 2016, 2(2), 13;

A Review of Process-Design Challenges for Industrial Fermentation of Butanol from Crude Glycerol by Non-Biphasic Clostridium pasteurianum

Department of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond Street, London, ON N6A 3K7, Canada
Institute of Biochemical Engineering, Technische Universität Braunschweig, Braunschweig 38106, Germany
Department of Biochemical Engineering, AVT—Aachener Verfahrenstechnik, RWTH Aachen University, Worringer Weg 1, Aachen 52074, Germany
Author to whom correspondence should be addressed.
Academic Editors: Badal C. Saha and Sangita Singh
Received: 5 April 2016 / Revised: 19 May 2016 / Accepted: 6 June 2016 / Published: 15 June 2016
(This article belongs to the Special Issue Industrial Biotechnology: An Emerging Area)
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Butanol, produced via traditional acetone-butanol-ethanol (ABE) fermentation, suffers from low yield and productivity. In this article, a non-ABE butanol production process is reviewed. Clostridium pasteurianum has a non-biphasic metabolism, alternatively producing 1,3-propanediol (PDO)-butanol-ethanol, referred to as PBE fermentation. This review discusses the advantages of PBE fermentation with an emphasis on applications using biodiesel-derived crude glycerol, currently an inexpensive and readily available feedstock. To address the process design challenges, various strategies have been employed and are examined and reviewed; genetic engineering and mutagenesis of C. pasteurianum, characterization and pretreatment of crude glycerol and various fermentation strategies such as bioreactor design and configuration, increasing cell density and in-situ product removal. Where research deficiencies exist for PBE fermentation, the process solutions as employed for ABE fermentation are reviewed and their suitability for PBE is discussed. Each of the obstacles against high butanol production has multiple solutions, which are reviewed with the end-goal of an integrated process for continuous high level butanol production and recovery using C. pasteurianum and biodiesel-derived crude glycerol. View Full-Text
Keywords: glycerol; Clostridium pasteurianum; butanol; PBE fermentation; in-situ recovery glycerol; Clostridium pasteurianum; butanol; PBE fermentation; in-situ recovery

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Sarchami, T.; Munch, G.; Johnson, E.; Kießlich, S.; Rehmann, L. A Review of Process-Design Challenges for Industrial Fermentation of Butanol from Crude Glycerol by Non-Biphasic Clostridium pasteurianum. Fermentation 2016, 2, 13.

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