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Fermentation 2016, 2(2), 10;

Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation

Microbiology Research Laboratory, Department of Basic Sciences, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan-173230, HP, India
Author to whom correspondence should be addressed.
Academic Editor: Marc Beauregard
Received: 19 January 2016 / Revised: 19 March 2016 / Accepted: 19 March 2016 / Published: 4 May 2016
(This article belongs to the Special Issue Industrial Biotechnology: An Emerging Area)
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A novel strain Rhizopus sp. C4 was isolated from compost for the production of pectinase. Cultivation of Rhizopus sp. C4 on orange peel substrate under various solid-state fermentation (SSF) conditions was evaluated for pectinase yield along with the enzyme activity profile as a potential, low-cost alternative to submerged-liquid fermentation. Response surface methodology (RSM) was employed to optimize various environmental parameters for pectinase production. Various parameters, namely temperature, moisture and incubation days, were studied statistically for a total of 20 runs using central composite design. The highest yield of the enzyme, i.e., 11.63 IU/mL, was obtained from 1:3.5 moisture ratios in 7 days at 30°C. The study demonstrated that optimization through RSM could improve the enzymatic characteristics and yield of the enzyme. View Full-Text
Keywords: pectinases; enzyme; optimization; pectinase; solid state fermentation pectinases; enzyme; optimization; pectinase; solid state fermentation

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Handa, S.; Sharma, N.; Pathania, S. Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation. Fermentation 2016, 2, 10.

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