This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Open AccessArticle
Scale-Up of a Two-Stage Anaerobic Digestion System: From Laboratory Reactor to Pilot Plant
by
Maria Isabella Lima Garção
Maria Isabella Lima Garção 1,*
,
Joachim Müller
Joachim Müller 2
and
Andreas Lemmer
Andreas Lemmer 1
1
State Institute of Agricultural Engineering and Bioenergy, University of Hohenheim, Garbenstr. 9, 70599 Stuttgart, Germany
2
Agricultural Engineering in the Tropics and Subtropics, University of Hohenheim, Garbenstr. 9, 70599 Stuttgart, Germany
*
Author to whom correspondence should be addressed.
Fermentation 2026, 12(6), 255; https://doi.org/10.3390/fermentation12060255 (registering DOI)
Submission received: 30 April 2026
/
Revised: 18 May 2026
/
Accepted: 22 May 2026
/
Published: 24 May 2026
Abstract
Two-stage anaerobic digestion systems are extensively researched for enhancing process stability and phase separation when processing complex organic materials. Scaling from laboratory setups to pilot plants necessitates engineering modifications to ensure operational feasibility. In this study, a laboratory-scale system comprising a 100 L horizontal CSTR and a packed-bed reactor was scaled up 100-fold. The design separates solid and liquid retention times, with fibers retained in the first stage while liquids and volatile fatty acids flow into the second. Fiber retention in the lab was achieved using a 100 µm sieve dividing the CSTR into two chambers, allowing prolonged lignocellulosic degradation. During scale-up, a filtration and recirculation system was introduced, able to return the fibers to the first reactor through a 1000 µm edge-gap filter, which separates liquids for the second reactor and recycles undegraded fibers. An economic analysis indicated a scale-up exponent of 0.396, indicating that unit costs decrease with plant size and demonstrating economies of scale. Laboratory-based mass balance estimates biogas production at approximately 16.3 m3 daily at the pilot scale, equivalent to 90 kWh. The modular system aims to be transferred to small farms, promoting cost-effective biogas from manure and local residues to support decentralized renewable energy in agriculture.
Share and Cite
MDPI and ACS Style
Lima Garção, M.I.; Müller, J.; Lemmer, A.
Scale-Up of a Two-Stage Anaerobic Digestion System: From Laboratory Reactor to Pilot Plant. Fermentation 2026, 12, 255.
https://doi.org/10.3390/fermentation12060255
AMA Style
Lima Garção MI, Müller J, Lemmer A.
Scale-Up of a Two-Stage Anaerobic Digestion System: From Laboratory Reactor to Pilot Plant. Fermentation. 2026; 12(6):255.
https://doi.org/10.3390/fermentation12060255
Chicago/Turabian Style
Lima Garção, Maria Isabella, Joachim Müller, and Andreas Lemmer.
2026. "Scale-Up of a Two-Stage Anaerobic Digestion System: From Laboratory Reactor to Pilot Plant" Fermentation 12, no. 6: 255.
https://doi.org/10.3390/fermentation12060255
APA Style
Lima Garção, M. I., Müller, J., & Lemmer, A.
(2026). Scale-Up of a Two-Stage Anaerobic Digestion System: From Laboratory Reactor to Pilot Plant. Fermentation, 12(6), 255.
https://doi.org/10.3390/fermentation12060255
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details
here.
Article Metrics
Article Access Statistics
For more information on the journal statistics, click
here.
Multiple requests from the same IP address are counted as one view.