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Article

Scale-Up of a Two-Stage Anaerobic Digestion System: From Laboratory Reactor to Pilot Plant

by
Maria Isabella Lima Garção
1,*,
Joachim Müller
2 and
Andreas Lemmer
1
1
State Institute of Agricultural Engineering and Bioenergy, University of Hohenheim, Garbenstr. 9, 70599 Stuttgart, Germany
2
Agricultural Engineering in the Tropics and Subtropics, University of Hohenheim, Garbenstr. 9, 70599 Stuttgart, Germany
*
Author to whom correspondence should be addressed.
Fermentation 2026, 12(6), 255; https://doi.org/10.3390/fermentation12060255 (registering DOI)
Submission received: 30 April 2026 / Revised: 18 May 2026 / Accepted: 22 May 2026 / Published: 24 May 2026

Abstract

Two-stage anaerobic digestion systems are extensively researched for enhancing process stability and phase separation when processing complex organic materials. Scaling from laboratory setups to pilot plants necessitates engineering modifications to ensure operational feasibility. In this study, a laboratory-scale system comprising a 100 L horizontal CSTR and a packed-bed reactor was scaled up 100-fold. The design separates solid and liquid retention times, with fibers retained in the first stage while liquids and volatile fatty acids flow into the second. Fiber retention in the lab was achieved using a 100 µm sieve dividing the CSTR into two chambers, allowing prolonged lignocellulosic degradation. During scale-up, a filtration and recirculation system was introduced, able to return the fibers to the first reactor through a 1000 µm edge-gap filter, which separates liquids for the second reactor and recycles undegraded fibers. An economic analysis indicated a scale-up exponent of 0.396, indicating that unit costs decrease with plant size and demonstrating economies of scale. Laboratory-based mass balance estimates biogas production at approximately 16.3 m3 daily at the pilot scale, equivalent to 90 kWh. The modular system aims to be transferred to small farms, promoting cost-effective biogas from manure and local residues to support decentralized renewable energy in agriculture.
Keywords: anaerobic digestion; scale-up; two-stage system; pilot scale; laboratory scale anaerobic digestion; scale-up; two-stage system; pilot scale; laboratory scale

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MDPI and ACS Style

Lima Garção, M.I.; Müller, J.; Lemmer, A. Scale-Up of a Two-Stage Anaerobic Digestion System: From Laboratory Reactor to Pilot Plant. Fermentation 2026, 12, 255. https://doi.org/10.3390/fermentation12060255

AMA Style

Lima Garção MI, Müller J, Lemmer A. Scale-Up of a Two-Stage Anaerobic Digestion System: From Laboratory Reactor to Pilot Plant. Fermentation. 2026; 12(6):255. https://doi.org/10.3390/fermentation12060255

Chicago/Turabian Style

Lima Garção, Maria Isabella, Joachim Müller, and Andreas Lemmer. 2026. "Scale-Up of a Two-Stage Anaerobic Digestion System: From Laboratory Reactor to Pilot Plant" Fermentation 12, no. 6: 255. https://doi.org/10.3390/fermentation12060255

APA Style

Lima Garção, M. I., Müller, J., & Lemmer, A. (2026). Scale-Up of a Two-Stage Anaerobic Digestion System: From Laboratory Reactor to Pilot Plant. Fermentation, 12(6), 255. https://doi.org/10.3390/fermentation12060255

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