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Open AccessArticle

Assessment of isomiR Discrimination Using Commercial qPCR Methods

Computational Medicine Center,, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA
Author to whom correspondence should be addressed.
Non-Coding RNA 2017, 3(2), 18;
Received: 24 January 2017 / Revised: 8 March 2017 / Accepted: 20 March 2017 / Published: 24 March 2017
We sought to determine whether commercial quantitative polymerase chain reaction (qPCR) methods are capable of distinguishing isomiRs: variants of mature microRNAs (miRNAs) with sequence endpoint differences. We used two commercially available miRNA qPCR methods to quantify miR-21-5p in both synthetic and real cell contexts. We find that although these miRNA qPCR methods possess high sensitivity for specific sequences, they also pick up background signals from closely related isomiRs, which influences the reliable quantification of individual isomiRs. We conclude that these methods do not possess the requisite specificity for reliable isomiR quantification. View Full-Text
Keywords: isomiR; miRNA; qPCR; short non-coding RNA isomiR; miRNA; qPCR; short non-coding RNA
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Magee, R.; Telonis, A.G.; Cherlin, T.; Rigoutsos, I.; Londin, E. Assessment of isomiR Discrimination Using Commercial qPCR Methods. Non-Coding RNA 2017, 3, 18.

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