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Article

MEF2C Directly Interacts with Pre-miRNAs and Distinct RNPs to Post-Transcriptionally Regulate miR-23a-miR-27a-miR-24-2 microRNA Cluster Member Expression

by
Estefanía Lozano-Velasco
1,2,†,
Carlos Garcia-Padilla
1,3,†,
Miguel Carmona-Garcia
1,
Alba Gonzalez-Diaz
1,
Angela Arequipa-Rendon
1,
Amelia E. Aranega
1,2 and
Diego Franco
1,2,*
1
Cardiovascular Development Group, Department of Experimental Biology, University of Jaen, 23071 Jaen, Spain
2
Fundación Medina, 18016 Granada, Spain
3
Department of Anatomy, Embryology and Zoology, School of Medicine, University of Extremadura, 06006 Badajoz, Spain
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Non-Coding RNA 2024, 10(3), 32; https://doi.org/10.3390/ncrna10030032
Submission received: 3 October 2023 / Revised: 11 May 2024 / Accepted: 15 May 2024 / Published: 17 May 2024
(This article belongs to the Section Small Non-Coding RNA)
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Abstract

Transcriptional regulation constitutes a key step in gene expression regulation. Myocyte enhancer factor 2C (MEF2C) is a transcription factor of the MADS box family involved in the early development of several cell types, including muscle cells. Over the last decade, a novel layer of complexity modulating gene regulation has emerged as non-coding RNAs have been identified, impacting both transcriptional and post-transcriptional regulation. microRNAs represent the most studied and abundantly expressed subtype of small non-coding RNAs, and their functional roles have been widely documented. On the other hand, our knowledge of the transcriptional and post-transcriptional regulatory mechanisms that drive microRNA expression is still incipient. We recently demonstrated that MEF2C is able to transactivate the long, but not short, regulatory element upstream of the miR-23a-miR-27a-miR-24-2 transcriptional start site. However, MEF2C over-expression and silencing, respectively, displayed distinct effects on each of the miR-23a-miR-27a-miR-24-2 mature cluster members without affecting pri-miRNA expression levels, thus supporting additional MEF2C-driven regulatory mechanisms. Within this study, we demonstrated a complex post-transcriptional regulatory mechanism directed by MEF2C in the regulation of miR-23a-miR-27a-miR-24-2 cluster members, distinctly involving different domains of the MEF2C transcription factor and the physical interaction with pre-miRNAs and Ksrp, HnRNPa3 and Ddx17 transcripts.
Keywords: Mef2c; microRNAs; RNPs Mef2c; microRNAs; RNPs

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MDPI and ACS Style

Lozano-Velasco, E.; Garcia-Padilla, C.; Carmona-Garcia, M.; Gonzalez-Diaz, A.; Arequipa-Rendon, A.; Aranega, A.E.; Franco, D. MEF2C Directly Interacts with Pre-miRNAs and Distinct RNPs to Post-Transcriptionally Regulate miR-23a-miR-27a-miR-24-2 microRNA Cluster Member Expression. Non-Coding RNA 2024, 10, 32. https://doi.org/10.3390/ncrna10030032

AMA Style

Lozano-Velasco E, Garcia-Padilla C, Carmona-Garcia M, Gonzalez-Diaz A, Arequipa-Rendon A, Aranega AE, Franco D. MEF2C Directly Interacts with Pre-miRNAs and Distinct RNPs to Post-Transcriptionally Regulate miR-23a-miR-27a-miR-24-2 microRNA Cluster Member Expression. Non-Coding RNA. 2024; 10(3):32. https://doi.org/10.3390/ncrna10030032

Chicago/Turabian Style

Lozano-Velasco, Estefanía, Carlos Garcia-Padilla, Miguel Carmona-Garcia, Alba Gonzalez-Diaz, Angela Arequipa-Rendon, Amelia E. Aranega, and Diego Franco. 2024. "MEF2C Directly Interacts with Pre-miRNAs and Distinct RNPs to Post-Transcriptionally Regulate miR-23a-miR-27a-miR-24-2 microRNA Cluster Member Expression" Non-Coding RNA 10, no. 3: 32. https://doi.org/10.3390/ncrna10030032

APA Style

Lozano-Velasco, E., Garcia-Padilla, C., Carmona-Garcia, M., Gonzalez-Diaz, A., Arequipa-Rendon, A., Aranega, A. E., & Franco, D. (2024). MEF2C Directly Interacts with Pre-miRNAs and Distinct RNPs to Post-Transcriptionally Regulate miR-23a-miR-27a-miR-24-2 microRNA Cluster Member Expression. Non-Coding RNA, 10(3), 32. https://doi.org/10.3390/ncrna10030032

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