Characterization Data for the Establishment of Scale-Up and Process Transfer Strategies between Stainless Steel and Single-Use Bioreactors
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reactor Setup and Operation Parameters
2.2. Experimental Procedure to Measure the Global Mixing Time
2.3. Experimental Procedure to Measure the Volumetric Mass Transfer Coefficient
2.4. Modeling of Mass Transfer Performance
3. Results
3.1. Mixing Time
3.2. Mass Transfer Performance and Modelling
3.3. Comparison of the Correlations for Different Scales and Reactors
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
DECHEMA | Gesellschaft für Chemische Technik und Biotechnologie e. V. | |
Eq | equation | |
FDA | Food and Drug Administration | |
MSE | mean squared error | |
OTR | oxygen transfer rate | |
Pb | pitched blade impeller | |
PBS | phosphate-buffered saline | |
RT | Rushton Turbine | |
Seg | segment impeller | |
STR | stirred-tank reactor | |
SUB | single-use bioreactor | |
TUHH | Hamburg University of Technology | |
Nomenclature | ||
Characteristic Numbers | ||
Re | stirrer Reynolds number | |
Greek Symbols | ||
empirical parameter | - | |
empirical parameter | - | |
empirical parameter | - | |
mixing time | s | |
dimensionless mixing time | - | |
density | kg m−3 | |
dynamic viscosity | Pa s | |
Roman Symbols | ||
a | specific area | m−1 |
c | concentration | - |
C | empirical parameter | - |
c* | saturation concentration | - |
d | diameter | m |
k | mass transfer coefficient | m s−1 |
n | stirring frequency | s−1 |
OTR | oxygen transfer rate | s−1 |
P | power | W |
t | time | s |
T | temperature | °C |
u | stirrer velocity | m s−1 |
V | volume | m3 |
v | gas velocity | m s−1 |
vvm | volumetric gassing rate | s−1 |
Subscripts | ||
e | electrode | |
L | liquid | |
mod. | modified | |
O2 | oxygen | |
R | impeller | |
S | superficial | |
tip | stirrer tip |
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System | Stainless Steel | Biostat STR® 2000 L | Biostat STR® 200 L |
---|---|---|---|
Working Volume | 8000 L–12,500 L | 1400 L–2000 L | 160 L–200 L |
Vessel Diameter | 2.000 m | 1.295 m | 0.595 m |
Impeller Type (bottom, top) | Rushton, Pitched-Blade | Rushton, Segment | Rushton, Segment |
Impeller Diameter | 0.665 m | 0.492 m | 0.225 m |
Angle Top Impeller | 45° | 30° | 30° |
Stirrer Speed Range | 30 rpm–80 rpm | 35 rpm–70 rpm | 62 rpm–150 rpm |
Aeration Range | 20 Lpm–250 Lpm | 10 Lpm–100 Lpm | 1 Lpm–20 Lpm |
Sparger Type | Open Tube | Ring (0.8 mm × 200) | Ring (0.8 mm × 20) |
Baffles | 4 × 200 mm | no baffles | no baffles |
Stainless Steel Stirred Tank | Biostat STR® 2000 L | Biostat STR® 200 L | |
---|---|---|---|
utip | |||
vvm | |||
V |
Coefficient | Stainless Steel Stirred Tank | Biostat STR® 2000 L | Biostat STR® 200 L |
---|---|---|---|
C | 25.84 | 29.40 | 25.58 |
α | 1.64 | 1.53 | 2.45 |
β | 0.92 | 0.69 | 0.50 |
γ | 0.81 | 0.55 | 0.11 |
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Bernemann, V.; Fitschen, J.; Leupold, M.; Scheibenbogen, K.-H.; Maly, M.; Hoffmann, M.; Wucherpfennig, T.; Schlüter, M. Characterization Data for the Establishment of Scale-Up and Process Transfer Strategies between Stainless Steel and Single-Use Bioreactors. Fluids 2024, 9, 115. https://doi.org/10.3390/fluids9050115
Bernemann V, Fitschen J, Leupold M, Scheibenbogen K-H, Maly M, Hoffmann M, Wucherpfennig T, Schlüter M. Characterization Data for the Establishment of Scale-Up and Process Transfer Strategies between Stainless Steel and Single-Use Bioreactors. Fluids. 2024; 9(5):115. https://doi.org/10.3390/fluids9050115
Chicago/Turabian StyleBernemann, Vincent, Jürgen Fitschen, Marco Leupold, Karl-Heinz Scheibenbogen, Marc Maly, Marko Hoffmann, Thomas Wucherpfennig, and Michael Schlüter. 2024. "Characterization Data for the Establishment of Scale-Up and Process Transfer Strategies between Stainless Steel and Single-Use Bioreactors" Fluids 9, no. 5: 115. https://doi.org/10.3390/fluids9050115
APA StyleBernemann, V., Fitschen, J., Leupold, M., Scheibenbogen, K. -H., Maly, M., Hoffmann, M., Wucherpfennig, T., & Schlüter, M. (2024). Characterization Data for the Establishment of Scale-Up and Process Transfer Strategies between Stainless Steel and Single-Use Bioreactors. Fluids, 9(5), 115. https://doi.org/10.3390/fluids9050115