Injectable Composite Systems of Gellan Gum:Alginate Microparticles in Pluronic Hydrogels for Bioactive Cargo Controlled Delivery: Optimization of Hydrogel Composition based on Rheological Behavior
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Microparticle Production
2.3. Hydrogel and Microparticle–Hydrogel Composite System
2.4. Rheological Characterization
2.5. In Vitro Degradation of Pluronic
2.6. In Vitro Drug Release
3. Results and Discussion
3.1. Sol–Gel Transition Temperature
3.2. Sol–Gel Transition of the Microparticle–Hydrogel Composite System
Mathematical Fittings of the Transition Temperatures
3.3. Frequency Sweeps at 37 °C
3.4. Flow Curves at 21 °C
3.5. Degradation of Pluronic Hydrogels
3.6. In Vitro MB-Release Profiles
3.7. Mathematical Fittings
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Tp | |||
---|---|---|---|
F127:F68 Ratio | m (°C/(wt.%)) | ||
14:6 | –0.4658 | 45.55 | 0.9799 |
15:5 | –0.4762 | 42.66 | 0.9764 |
16:4 | –0.4564 | 38.74 | 0.8805 |
17:3 | –0.4668 | 35.38 | 0.9616 |
Ti | |||
F127:F68 Ratio | m (°C/(wt.%)) | (°C) | |
14:6 | –0.5886 | 41.91 | 0.8804 |
15:5 | –0.4182 | 38.16 | 0.8413 |
16:4 | –0.4646 | 35.27 | 0.8341 |
17:3 | –0.4900– | 31.72 | 0.9900 |
Batches (g)/pH | 16:4 5% | 17:3 5% | 17:3 2% | Microparticles | ||||
---|---|---|---|---|---|---|---|---|
pH 6.5 | pH 7.4 | pH 6.5 | pH 7.4 | pH 6.5 | pH 7.4 | pH 6.5 | ||
KP Tlag | k | 21.150 | 22.533 | 20.995 | 20.710 | 23.236 | 21.849 | 30.191 |
n | 0.349 | 0.309 | 0.331 | 0.298 | 0.312 | 0.325 | 0.240 | |
Tlag | 1.938 | 2.923 | 3.868 | 3.896 | 3.959 | 2.957 | 2.957 | |
R2adj | 0.929 | 0.956 | 0.965 | 0.956 | 0.955 | 0.961 | 0.955 | |
PS Tlag | k1 | 10.316 | 12.253 | 9.105 | 7.820 | 8.011 | 11.780 | 13.744 |
k2 | –0.245 | –0.389 | –0.205 | –0.178 | –0.158 | –0.345 | –0.446 | |
m | 0.691 | 0.593 | 0.658 | 0.670 | 0.730 | 0.606 | 0.557 | |
Tlag | 0.835 | 1.746 | 2.330 | 1.791 | 1.872 | 1.894 | 0.893 | |
R2adj | 0.995 | 0.994 | 0.997 | 0.996 | 0.989 | 0.991 | 0.990 |
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Carrêlo, H.; Escoval, A.R.; Soares, P.I.P.; Borges, J.P.; Cidade, M.T. Injectable Composite Systems of Gellan Gum:Alginate Microparticles in Pluronic Hydrogels for Bioactive Cargo Controlled Delivery: Optimization of Hydrogel Composition based on Rheological Behavior. Fluids 2022, 7, 375. https://doi.org/10.3390/fluids7120375
Carrêlo H, Escoval AR, Soares PIP, Borges JP, Cidade MT. Injectable Composite Systems of Gellan Gum:Alginate Microparticles in Pluronic Hydrogels for Bioactive Cargo Controlled Delivery: Optimization of Hydrogel Composition based on Rheological Behavior. Fluids. 2022; 7(12):375. https://doi.org/10.3390/fluids7120375
Chicago/Turabian StyleCarrêlo, Henrique, André R. Escoval, Paula I. P. Soares, João P. Borges, and Maria Teresa Cidade. 2022. "Injectable Composite Systems of Gellan Gum:Alginate Microparticles in Pluronic Hydrogels for Bioactive Cargo Controlled Delivery: Optimization of Hydrogel Composition based on Rheological Behavior" Fluids 7, no. 12: 375. https://doi.org/10.3390/fluids7120375