Dicarboxylic Amino Acid Permease 7219 Regulates Fruiting Body Type of Auricularia heimuer
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Culture Conditions
2.2. Nucleic Acid Extraction
2.3. Primary Mapping of Fruiting Body Type Trait
2.4. Fine Mapping of Fruiting Body Type Trait and SCL-18 Alignment
2.5. Transcriptome Analysis, qRT-PCR Validation, and Candidate Gene Screening
2.6. Polymorphism Analysis for Promoter Base Detection of Candidate Genes
2.7. Statistical Analysis
3. Results
3.1. Classification of Fruiting Body Types of Locational Groups
3.2. Localization of the Fruiting Body Type Locus
3.3. SCL-18 Alignment and Screening of Candidate Genes
3.4. Nucleotide Sequence of the g7219 Gene
3.5. Polymorphism in the Promoter of the g7219 Gene
4. Discussion
4.1. Primary Positioning
4.2. Increased Density of Genetic Linkage Map
4.3. Fine Positioning of Fruiting Body Type Locus of A. heimuer
4.4. Physical Location of Fruiting Body Type Markers and Functional Prediction of Candidate Genes
4.5. Polymorphism of Candidate Genes in Strains with Different Fruiting Body Types
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Number | Name | Fruiting Body Pattern | Number | Name | Fruiting Body Pattern |
---|---|---|---|---|---|
A1 | Heiwei 981 | CL | A13 | Heimeur 1 | CH |
A2 | Hei 793 | CH | A14 | Qihei No. 1 | CL |
A5 | Zhejiang Ear 1 | CH | A15 | Heier 5 | CH |
A6 | Zheer 1 | CL | A17 | Heier 6 | CL |
A8 | H10 | CH | A18 | Qihei No. 2 | CH |
A9 | Yante 3 | CL | A19 | Fengshou 2 | CL |
A10 | Yante 5 | CL | A22 | JiAU 2 | CL |
A12 | Xinke | CH |
Fruiting Body Pattern | Cluster Type (CL) | Chrysanthemum Type (CH) |
---|---|---|
parental strain | A14 | A18 |
cross population | C3, C6, C7, C8, C11, C21, C22, C23, C25, C27, C28, C29, C30, C33, C34, C35, C37, C38, C39, C40, C42, C45, C55, C58, C60, C63, C68, C72, C77, C79, C80, C85, C87, C89, C91, C95, C96, C106, C107, C109, C112, C119, C127, C129, C133, C139, C149, C153, C157, C160, C161, C163, C164, C166, C167, C169, C171, C174, C175, C183, C184, C185, 186, C188, C193, C199, C201 | C1, C2, C12, C15, C17, C18, C19, C20, C32, C50, C51, C57, C61, C64, C65, C66, C68, C70, C71, C73, C74, C75, C76, C78, C81, C82, C86, C88, C90, C92, C93, C94, C97, C98, C99, C101, C102, C103, C105, C110, C114, C115, C118, C120, C121, C123, C126, C128, C132, C134, C135, C136, C146, C150, C152, C154, C165, C168, C170, C172, C178, C179, C180, C181, C187, C190, C191, C192, C197, C198, C202 |
Strain | BioProject (PRJNA) | Contingent Count (N50 kb) | Total Length (Mb) | Scaffold Count (N50 Mb) | GC Count (%) |
---|---|---|---|---|---|
B14-8 | 382471 | 1071 (121.22) | 43.57 | 534 (0.27) | 57 |
Dai13782 | 382748 | 114 (1006.44) | 49.76 | 103 (1.35) | 57 |
Scheme | Characteristics | Secondary Structure | |||||
---|---|---|---|---|---|---|---|
Isoelectric Point pI | Molecular Weight Mw(kD) | Instability Index | Hydrophilic | α-Helix (%) | β-Angle of Rotation (%) | Irregular Curl | |
A14 | 5.90 | 28.35 | 39.43 | 0.904 | 48.03 | 26.38 | 22.83 |
A18 | 5.37 | 28.34 | 40.05 | 0.911 | 49.61 | 21.65 | 24.41 |
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Lu, J.; Lu, L.; Yao, F.; Fang, M.; Ma, X.; Meng, J.; Shao, K. Dicarboxylic Amino Acid Permease 7219 Regulates Fruiting Body Type of Auricularia heimuer. J. Fungi 2023, 9, 876. https://doi.org/10.3390/jof9090876
Lu J, Lu L, Yao F, Fang M, Ma X, Meng J, Shao K. Dicarboxylic Amino Acid Permease 7219 Regulates Fruiting Body Type of Auricularia heimuer. Journal of Fungi. 2023; 9(9):876. https://doi.org/10.3390/jof9090876
Chicago/Turabian StyleLu, Jia, Lixin Lu, Fangjie Yao, Ming Fang, Xiaoxu Ma, Jingjing Meng, and Kaisheng Shao. 2023. "Dicarboxylic Amino Acid Permease 7219 Regulates Fruiting Body Type of Auricularia heimuer" Journal of Fungi 9, no. 9: 876. https://doi.org/10.3390/jof9090876