Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores
Abstract
:1. Introduction
2. Materials and Methods
2.1. Clinical and Environmental Isolates
2.2. Molecular Identification
2.3. Generation of the Local Database
2.3.1. Culture
2.3.2. Protein Extraction
2.3.3. Spectral Acquisition
2.3.4. Quality Control
2.3.5. Creation of MSP
2.4. Evaluation of MSP Library Performance
3. Results
3.1. Interest of Culture Media
3.2. Characteristics of the Local Database
3.3. Evaluation of the Local Database Performance and Comparison with the Bruker Database
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Species | Isolate Number | Origins |
---|---|---|
Aspergillus amoenus | 1 (E) | Mold-damaged home |
Aspergillus creber | 2 (E) | Cancer treatment center |
3 (E) | Cancer treatment center | |
4 (E) | Serpula lacrymans-damaged home | |
5 (C) | Skin scales of right foot | |
6 (C) | Skin scales of right foot | |
7 (C) | Sputum | |
8 (E) | Mold-damaged home | |
9 (E) | Mold-damaged home | |
10 (E) | Mold-damaged home | |
11 (E) | Mold-damaged home | |
Aspergillus fructus | 12 (C) | Nail of big toe |
Aspergillus jensenii | 13 (C) | Scalp |
14 (C) | Bronchoalveolar lavage fluid | |
15 (C) | Bronchoalveolar lavage fluid | |
16 (E) | Mold-damaged home | |
Aspergillus protuberus | 17 (E) | Cancer treatment center |
Aspergillus puulaauensis | 18 (C) | Armpit skin |
Aspergillus sydowii | 19 (C) | Bronchoalveolar lavage fluid |
20 (C) | Bronchoalveolar lavage fluid | |
21 (C) | External auditory canal | |
22 (C) | Bronchoalveolar lavage fluid | |
23 (C) | Bronchoalveolar lavage fluid | |
24 (C) | Bronchoalveolar lavage fluid | |
25 (C) | Bronchoalveolar lavage fluid | |
26 (C) | Nail | |
27 (C) | Bronchoalveolar lavage fluid | |
28 (C) | Bronchoalveolar lavage fluid | |
29 (C) | Bronchoalveolar lavage fluid | |
Aspergillus tabacinus | 30 (C) | Sputum (cystic fibrosis) |
Species | Isolate Number | Culture Medium | Number of MSPs Obtained | ||||||
---|---|---|---|---|---|---|---|---|---|
SAB | MEA | CZA | LIQUID SAB | ||||||
First Attempt | Second Attempt | Third Attempt | First Attempt | Second Attempt | First Attempt | First Attempt | |||
Aspergillus amoenus | 1 I | 1 MSP | / | / | 2 MSPs | / | 0 MSP | / | 3 |
Aspergillus creber | 2 (E) | 0 MSP | 0 MSP | 1 MSP | 0 MSP | 0 MSP | 0 MSP | 1 MSP | 2 |
3 (E) | 0 MSP | 0 MSP | 1 MSP | 1 MSP | / | / | / | 2 | |
4 (E) | 0 MSP | 0 MSP | 1 MSP | 0 MSP | 0 MSP | 0 MSP | 1 MSP | 2 | |
5 (C) | 0 MSP | 0 MSP | 1 MSP | 1 MSP | / | / | / | 2 | |
6 (C) | 1 MSP | / | / | 0 MSP | 0 MSP | 0 MSP | 0 MSP | 1 | |
7 (C) | 0 MSP | 0 MSP | 1 MSP | 0 MSP | 1 MSP | 0 MSP | / | 2 | |
8 (E) | 1 MSP | / | / | 0 MSP | 0 MSP | 0 MSP | 0 MSP | 1 | |
9 (E) | 0 MSP | 0 MSP | 1 MSP | 1 MSP | / | / | / | 2 | |
10 (E) | 1 MSP | / | / | 2 MSPs | / | / | / | 3 | |
11 (E) | 0 MSP | 0 MSP | 1 MSP | 1 MSP | / | / | / | 2 | |
Aspergillus fructus | 12 (C) | 1 MSP | / | / | 1 MSP | / | 0 MSP | / | 2 |
Aspergillus jensenii | 13 (C) | 1 MSP | / | / | 1 MSP | / | / | / | 2 |
14 (C) | 0 MSP | 0 MSP | 1 MSP | 0 MSP | 0 MSP | 0 MSP | 0 MSP | 1 | |
15 (C) | 1 MSP | / | / | 1 MSP | / | / | / | 2 | |
16 (E) | 0 MSP | 0 MSP | 1 MSP | 0 MSP | 0 MSP | 0 MSP | 0 MSP | 1 | |
Aspergillus protuberus | 17 (E) | 0 MSP | 0 MSP | 1 MSP | 0 MSP | 0 MSP | 0 MSP | 0 MSP | 1 |
Aspergillus puulaauensis | 18 (C) | 0 MSP | 0 MSP | 1 MSP | 1 MSP | / | 0 MSP | 1 MSP | 3 |
Aspergillus sydowii | 19 (C) | 2 MSPs | / | / | 1 MSP | / | / | / | 3 |
20 (C) | 1 MSP | / | / | 1 MSP | / | / | / | 2 | |
21 (C) | 1 MSP | / | / | 1 MSP | / | / | / | 2 | |
22 (C) | 1 MSP | / | / | 1 MSP | / | / | / | 2 | |
23 (C) | 2 MSPs | / | / | 0 MSP | 0 MSP | 0 MSP | / | 2 | |
24 (C) | 1 MSP | / | / | 1 MSP | / | / | / | 2 | |
25 (C) | 1 MSP | / | / | 1 MSP | / | / | / | 2 | |
26 (C) | 1 MSP | / | / | 1 MSP | / | / | / | 2 | |
27 (C) | 1 MSP | / | / | 1 MSP | / | / | / | 2 | |
28 (C) | 1 MSP | / | / | 1 MSP | / | / | / | 2 | |
29 (C) | 1 MSP | / | / | 1 MSP | / | / | / | 2 | |
Aspergillus tabacinus | 30 (C) | 1 MSP | / | / | 1 MSP | / | 0 MSP | / | 2 |
Species | Isolates Number | Bruker Database | Local Database | Total Number of Profiles (/10) | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Log Score ≥ 2.00 | Number of Profiles | 1.70 ≤ Log Score < 2.00 | Number of Profiles | Log Score ≥ 2.00 | Number of Profiles | 1,70 ≤ Log Score < 2.00 | Number of Profiles | ||||
Aspergillus amoenus | 1 (E) | Aspergillus versicolor 120227_14 ETL | 1 | 0 | Aspergillus amoenus | 2 | 0 | 10 | |||
Aspergillus versicolor 392 UGB | 1 | Aspergillus tabacinus | 1 | ||||||||
Aspergillus versicolor D_16_256_8_1 LLH | 1 | Aspergillus fructus | 2 | ||||||||
Aspergillus versicolor 1343 MPA | 1 | Aspergillus protuberus | 1 | ||||||||
Aspergillus creber | 2 (E) | Aspergillus versicolor F51 LLH | 1 | 0 | Aspergillus creber | 7 | 0 | 10 | |||
Aspergillus versicolor DSM 63292 DSM | 1 | Aspergillus puulaauensis | 1 | ||||||||
3 (E) | 0 | 0 | Aspergillus creber | 9 | 0 | 10 | |||||
Aspergillus puulaauensis | 1 | ||||||||||
4 (E) | Aspergillus versicolor F51 LLH | 1 | 0 | Aspergillus creber | 8 | 0 | 10 | ||||
Aspergillus puulaauensis | 1 | ||||||||||
5 (C) | 0 | 0 | Aspergillus creber | 9 | 0 | 10 | |||||
Aspergillus puulaauensis | 1 | ||||||||||
6 (C) | Aspergillus versicolor F51 LLH | 1 | 0 | Aspergillus creber | 8 | 0 | 10 | ||||
Aspergillus puulaauensis | 1 | ||||||||||
7 (C) | 0 | 0 | Aspergillus creber | 10 | 0 | 10 | |||||
8 (E) | Aspergillus versicolor F51 LLH | 1 | 0 | Aspergillus creber | 9 | 0 | 10 | ||||
9 (E) | Aspergillus versicolor F51 LLH | 1 | 0 | Aspergillus creber | 9 | 0 | 10 | ||||
10 (E) | 0 | 0 | Aspergillus creber | 10 | 0 | 10 | |||||
11 (E) | Aspergillus versicolor F51 LLH | 1 | 0 | Aspergillus creber | 8 | 0 | 10 | ||||
Aspergillus puulaauensis | 1 | ||||||||||
Aspergillus fructus | 12 (C) | Aspergillus versicolor 120227_14 ETL | 1 | Aspergillus versicolor 1343 MPA | 1 | Aspergillus fructus | 2 | Aspergillus protuberus | 1 | 10 | |
Aspergillus versicolor 392 UGB | 1 | Aspergillus amoenus | 1 | Aspergillus puulaauensis | 1 | ||||||
Aspergillus versicolor D_16_256_8_1 LLH | 1 | Aspergillus tabacinus | 1 | ||||||||
Aspergillus jensenii | 13 (C) | 0 | 0 | Aspergillus jensenii | 4 | 0 | 10 | ||||
Aspergillus creber | 3 | ||||||||||
Aspergillus puulaauensis | 3 | ||||||||||
14 (C) | 0 | 0 | Aspergillus jensenii | 3 | Aspergillus jensenii | 1 | 10 | ||||
Aspergillus creber | 2 | Aspergillus creber | 3 | ||||||||
Aspergillus puulaauensis | 1 | ||||||||||
15 (C) | 0 | 0 | Aspergillus jensenii | 5 | 0 | 10 | |||||
Aspergillus creber | 4 | ||||||||||
Aspergillus puulaauensis | 1 | ||||||||||
16 (E) | 0 | 0 | Aspergillus jensenii | 3 | Aspergillus jensenii | 2 | 10 | ||||
Aspergillus creber | 2 | Aspergillus creber | 2 | ||||||||
Aspergillus puulaauensis | 1 | ||||||||||
Aspergillus protuberus | 17 (E) | 0 | Aspergillus versicolor 120227_14 ETL | 1 | Aspergillus protuberus | 1 | Aspergillus fructus | 2 | 10 | ||
Aspergillus tabacinus | 1 | Aspergillus sydowii | 3 | ||||||||
Aspergillus amoenus | 1 | Aspergillus puulaauensis | 1 | ||||||||
Aspergillus puulaauensis | 18 (C) | Aspergillus versicolor F51 LLH | 1 | 0 | Aspergillus puulaauensis | 3 | 0 | 10 | |||
Aspergillus jensenii | 2 | ||||||||||
Aspergillus creber | 4 | ||||||||||
Aspergillus sydowii | 19 (C) | Aspergillus versicolor 1343 MPA | 1 | 0 | Aspergillus sydowii | 8 | Aspergillus sydowii | 1 | 10 | ||
20 (C) | 0 | 0 | Aspergillus sydowii | 9 | Aspergillus sydowii | 1 | 10 | ||||
21 (C) | 0 | 0 | Aspergillus sydowii | 9 | Aspergillus sydowii | 1 | 10 | ||||
22 (C) | Aspergillus versicolor 1343 MPA | 1 | 0 | Aspergillus sydowii | 6 | Aspergillus sydowii | 3 | 10 | |||
23 (C) | 0 | Aspergillus sydowii 2008_141783 MUZ | 1 | Aspergillus sydowii | 2 | Aspergillus sydowii | 7 | 10 | |||
24 (C) | 0 | Aspergillus versicolor 1343 MPA | 1 | Aspergillus sydowii | 5 | Aspergillus sydowii | 4 | 10 | |||
25 (C) | 0 | Aspergillus sydowii 2008_141783 MUZ | 1 | Aspergillus sydowii | 8 | Aspergillus sydowii | 1 | 10 | |||
26 (C) | 0 | 0 | Aspergillus sydowii | 10 | 0 | 10 | |||||
27 (C) | Aspergillus versicolor 1343 MPA | 1 | 0 | Aspergillus sydowii | 8 | 0 | 10 | ||||
Aspergillus amoenus | 1 | ||||||||||
28 (C) | 0 | Aspergillus versicolor 1343 MPA | 1 | 0 | Aspergillus sydowii | 8 | 10 | ||||
Aspergillus versicolor 2009_137364 MUZ | 1 | ||||||||||
29 (C) | Aspergillus versicolor 1343 MPA | 1 | 0 | Aspergillus sydowii | 9 | 0 | 10 | ||||
Aspergillus tabacinus | 30 (C) | Aspergillus versicolor 120227_14 ETL | 1 | 0 | Aspergillus tabacinus | 2 | Aspergillus fructus | 1 | 10 | ||
Aspergillus amoenus | 1 | Aspergillus jensenii | 2 | ||||||||
Aspergillus protuberus | 1 | Aspergillus amoenus | 1 | ||||||||
Aspergillus fructus | 1 | ||||||||||
Total number of profiles | 18 | 9 | 225 | 48 | 300 |
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Jomat, O.; Géry, A.; Leudet, A.; Capitaine, A.; Garon, D.; Bonhomme, J. Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores. J. Fungi 2023, 9, 868. https://doi.org/10.3390/jof9090868
Jomat O, Géry A, Leudet A, Capitaine A, Garon D, Bonhomme J. Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores. Journal of Fungi. 2023; 9(9):868. https://doi.org/10.3390/jof9090868
Chicago/Turabian StyleJomat, Océane, Antoine Géry, Astrid Leudet, Agathe Capitaine, David Garon, and Julie Bonhomme. 2023. "Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores" Journal of Fungi 9, no. 9: 868. https://doi.org/10.3390/jof9090868
APA StyleJomat, O., Géry, A., Leudet, A., Capitaine, A., Garon, D., & Bonhomme, J. (2023). Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores. Journal of Fungi, 9(9), 868. https://doi.org/10.3390/jof9090868