Assessment of Fungal Succession in Decomposing Swine Carcasses (Sus scrofa L.) Using DNA Metabarcoding
Abstract
:1. Introduction
1.1. Decomposition in Forensic Investigations
1.2. Fungi in Animal Decomposition
1.3. DNA Metabarcoding of Fungi
2. Materials and Methods
2.1. Field Experiment Design
2.2. DNA Extraction
2.3. PCR
2.4. High-Throughput Sequencing (Next Generation Sequencing, NGS)
2.5. Data Analysis
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer Name | Primer Sequence, from 5′ |
---|---|
tag_1F | CACACGATCT GTGARTCATCGAATCTTTG |
tag_1R | CACACGCTGT TCCTCCGCTTATTGATATGC |
tag_4F | CACATAGTCT GTGARTCATCGAATCTTTG |
tag_4R | CACATGTCGT TCCTCCGCTTATTGATATGC |
tag_5F | CACATGACTT GTGARTCATCGAATCTTTG |
tag_5R | CACGCAGCAT TCCTCCGCTTATTGATATGC |
tag_6F | CACGATCAGT GTGARTCATCGAATCTTTG |
tag_6R | CACTAGCGCT TCCTCCGCTTATTGATATGC |
tag_7F | CACGTGCTCT GTGARTCATCGAATCTTTG |
tag_7R | CACTATGCAT TCCTCCGCTTATTGATATGC |
tag_8F | CACTATAGCT GTGARTCATCGAATCTTTG |
tag_8R | CACTCACACT TCCTCCGCTTATTGATATGC |
tag_9F | CACTATGTGT GTGARTCATCGAATCTTTG |
tag_9R | CACTCTGAGT TCCTCCGCTTATTGATATGC |
tag_10F | CACTCAGAGT GTGARTCATCGAATCTTTG |
tag_10R | CACTGATCAT TCCTCCGCTTATTGATATGC |
tag_11F | CACTCTCACT GTGARTCATCGAATCTTTG |
tag_11R | CACTGTATGT TCCTCCGCTTATTGATATGC |
tag_12F | CACTGCTACT GTGARTCATCGAATCTTTG |
tag_12R | CAGACATAGT TCCTCCGCTTATTGATATGC |
tag_13F | CAGACAGTGT GTGARTCATCGAATCTTTG |
tag_13R | CAGACTATGT TCCTCCGCTTATTGATATGC |
tag_14F | CAGACATCTT GTGARTCATCGAATCTTTG |
tag_14R | CAGAGCTCAT TCCTCCGCTTATTGATATGC |
tag_15F | CAGAGACGCT GTGARTCATCGAATCTTTG |
tag_15R | CAGATACACT TCCTCCGCTTATTGATATGC |
tag_16F | CAGAGCTCGT GTGARTCATCGAATCTTTG |
tag_16R | CAGATGCTAT TCCTCCGCTTATTGATATGC |
tag_17F | CAGAGTATGT GTGARTCATCGAATCTTTG |
tag_17R | CAGCACGACT TCCTCCGCTTATTGATATGC |
tag_18F | CAGATACAGT GTGARTCATCGAATCTTTG |
tag_18R | CAGCACTCGT TCCTCCGCTTATTGATATGC |
tag_20F | CAGCACTATT GTGARTCATCGAATCTTTG |
tag_20R | CAGCTGACGT TCCTCCGCTTATTGATATGC |
tag_21F | CAGCGATACT GTGARTCATCGAATCTTTG |
tag_21R | CAGTATCTCT TCCTCCGCTTATTGATATGC |
tag_22F | CAGCTAGATT GTGARTCATCGAATCTTTG |
tag_22R | CAGTCAGTCT TCCTCCGCTTATTGATATGC |
tag_23F | CAGCTCACTT GTGARTCATCGAATCTTTG |
tag_23R | CAGTCGTGCT TCCTCCGCTTATTGATATGC |
Fungal Species | Stage | Indicator Value | p-Value | Guild |
---|---|---|---|---|
Alternaria metachromatica | Zero | 0.450013 | 0.029 | |
Apiotrichum mycotoxinovorans | Zero | 0.3204 | 0.005 | |
Ascomycota sp. | Zero | 0.30861 | 0.004 | |
Beauveria bassiana | Zero | 0.381168 | 0.044 | Animal pathogen |
Cladosporium tenuissimum | Zero | 0.279405 | 0.029 | Endophyte |
Cryptococcus uniguttulatus | Zero | 0.3 | 0.037 | |
Cystobasidium pallidum | Zero | 0.616563 | 0.016 | |
Cystofilobasidium capitatum | Fresh | 0.24132 | 0.002 | |
Erysiphe corylacearum | Fresh | 0.558174 | 0.004 | |
Exophiala salmonis | Bloat | 0.223837 | 0.021 | Endophyte |
Filobasidium sp. | Bloat | 0.173077 | 0.032 | |
Gibellulopsis piscis | Bloat | 0.370083 | 0.038 | |
Jaminaea sp. | Bloat | 0.404757 | 0.003 | |
Knufia tsunedae | Bloat | 0.329308 | 0.028 | Soil Saprotroph |
Lachancea thermotolerans | Bloat | 0.325791 | 0.003 | |
Leucosporidium intermedium | Bloat | 0.278229 | 0.016 | |
Malassezia restricta | Bloat | 0.341931 | 0.035 | Animal pathogen |
Metarhizium anisopliae | Bloat | 0.229167 | 0.003 | |
Mortierella alpina | Decay | 0.352564 | 0.001 | |
Mortierella elongata | Decay | 0.526913 | 0.016 | |
Mucor racemosus | Decay | 0.25907 | 0.006 | Endophyte |
Neophaeococcomyces catenatus | Decay | 0.264503 | 0.043 | |
Phallus rugulosus | Decay | 0.214286 | 0.001 | |
Pleosporales sp. | Decay | 0.25 | 0.011 | |
Powellomyces sp. | Decay | 0.351021 | 0.004 | Plant pathogen |
Pseudomicrostroma phylloplanum | Decay | 0.361429 | 0.03 | |
Pyrenochaetopsis leptospora | Decay | 0.242138 | 0.001 | |
Schwanniomyces occidentalis | Decay | 0.734502 | 0.019 | |
Tausonia pullulans | Decay | 0.296296 | 0.003 | Saprotroph |
Trichoderma harzianum | Decay | 0.480422 | 0.036 | Endophyte-Fungal Parasite-Plant Pathogen |
Trichomeriaceae sp. | Decay | 0.333857 | 0.015 | Epiphyte |
Ustilago nunavutica | Decay | 0.457515 | 0.001 | |
Wallemia muriae | Decay | 0.257678 | 0.001 | |
Wallemia tropicalis | Decay | 0.654491 | 0.002 | |
Xylariales sp. | Active decay | 0.267912 | 0.027 |
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Share and Cite
Gemmellaro, M.D.; Lorusso, N.S.; Domke, R.; Kovalska, K.M.; Hashim, A.; Arevalo Mojica, M.; O’Connor, A.J.; Patel, U.; Pate, O.; Raise, G.; et al. Assessment of Fungal Succession in Decomposing Swine Carcasses (Sus scrofa L.) Using DNA Metabarcoding. J. Fungi 2023, 9, 866. https://doi.org/10.3390/jof9090866
Gemmellaro MD, Lorusso NS, Domke R, Kovalska KM, Hashim A, Arevalo Mojica M, O’Connor AJ, Patel U, Pate O, Raise G, et al. Assessment of Fungal Succession in Decomposing Swine Carcasses (Sus scrofa L.) Using DNA Metabarcoding. Journal of Fungi. 2023; 9(9):866. https://doi.org/10.3390/jof9090866
Chicago/Turabian StyleGemmellaro, M. Denise, Nicholas Steven Lorusso, Rachel Domke, Kristina M. Kovalska, Ayesha Hashim, Maria Arevalo Mojica, Amanda Joy O’Connor, Urvi Patel, Olivia Pate, Gloria Raise, and et al. 2023. "Assessment of Fungal Succession in Decomposing Swine Carcasses (Sus scrofa L.) Using DNA Metabarcoding" Journal of Fungi 9, no. 9: 866. https://doi.org/10.3390/jof9090866