Species Identification and In Vitro Antifungal Susceptibility of Paecilomyces/Purpureocillium Species Isolated from Clinical Respiratory Samples: A Multicenter Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Isolates
2.2. Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry Identification
2.3. Molecular Identification and Phylogenetic Analysis
2.4. Antifungal Susceptibility Testing
3. Results
3.1. Isolates and Samples
3.2. Species Identification
3.3. Antifungal Susceptibility
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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CBS No | Gene Bank Accession N° | ||
---|---|---|---|
Species | ITS [20] | D1D2 [20] | |
CBS 372.70 T | Paecilomyces maximus | MH859719.1 | MH871470.1 |
CBS 371.70 T | Paecilomyces maximus | MH859718.1 | MH871469.1 |
CBS 339.51 | Paecilomyces variotii | MH856887.1 | MH868409.1 |
CBS 338.51 | Paecilomyces variotii | MH856886.1 | MH868408.1 |
CBS 284.48 T | Paecilomyces divaricatus | MH856344.1 | MH867896.1 |
CBS 368.70 | Paecilomyces dactylethromorphus | MH859715.1 | MH871467.1 |
CBS 129474 | Purpureocillium lilacinum | MH865347.1 | MH876802.1 |
CBS 346.51 | Purpureocillium lilacinum | MH856891.1 | MH868413.1 |
CBS 528.71 T | Thermoascus thermophilus | MH860254.1 | MH872018.1 |
CBS 128677 T | Purpureocillium lavendulum | MH864976.1 | MH876429.1 |
CBS 182.27 T | Marquandomyces marquandii | MH854923.1 | MH866418.1 |
CBS 156.65 | Cordyceps farinosa | MH858528.1 | MH870163.1 |
Species and Drug | Range | GM | MIC50/MEC50 | MIC90/MEC90 |
---|---|---|---|---|
(mg/L) | (mg/L) | (mg/L) | (mg/L) | |
Purpureocillium lilacinum (n = 28) | ||||
VRC | 0.06–4 | 0.320 | 0.25 | 0.5 |
ITC | 0.5–8 | 1.414 | 1 | 2 |
PCZ | 0.06–0.5 | 0.262 | 0.25 | 0.5 |
ISA | 0.125–4 | 0.305 | 0.25 | 1 |
CAS | 8–16 | 13.125 | 16 | 16 |
MCF | 16–16 | 16 | 16 | 16 |
AND | 8–16 | 13.792 | 16 | 16 |
AMB | 16–16 | 16 | 16 | 16 |
Paecilomyces variotii stricto sensu (n = 26) | ||||
VRC | 0.25–16 | 4.108 | 4 | 16 |
ITC | 0.015–0.5 | 0.130 | 0.125 | 0.25 |
PCZ | 0.015–0.25 | 0.080 | 0.06 | 0.125 |
ISA | 0.06–16 | 5.499 | 8 | 16 |
CAS | 0.06–16 | 4.095 | 8 | 8 |
MCF | 0.015–0.03 | 0.015 | 0.015 | 0.015 |
AND | 0.015–0.015 | 0.015 | 0.015 | 0.015 |
AMB | 0.06–0.5 | 0.171 | 0.25 | 0.25 |
Paecilomyces maximus (n = 16) | ||||
VRC | 0.5–16 | 8.775 | 8 | 16 |
ITC | 0.125–1 | 0.379 | 0.5 | 0.5 |
PCZ | 0.125–0.5 | 0.228 | 0.25 | 0.25 |
ISA | 4–16 | 12.699 | 16 | 16 |
CAS | 0.015–16 | 0.746 | 1 | 8 |
MCF | 0.015–0.06 | 0.016 | 0.015 | 0.015 |
AND | 0.015–0.015 | 0.015 | 0.015 | 0.015 |
AMB | 0.125–0.5 | 0.262 | 0.25 | 0.5 |
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Monpierre, L.; Aït-Ammar, N.; Valsecchi, I.; Normand, A.-C.; Guitard, J.; Riat, A.; Huguenin, A.; Bonnal, C.; Sendid, B.; Hasseine, L.; et al. Species Identification and In Vitro Antifungal Susceptibility of Paecilomyces/Purpureocillium Species Isolated from Clinical Respiratory Samples: A Multicenter Study. J. Fungi 2022, 8, 684. https://doi.org/10.3390/jof8070684
Monpierre L, Aït-Ammar N, Valsecchi I, Normand A-C, Guitard J, Riat A, Huguenin A, Bonnal C, Sendid B, Hasseine L, et al. Species Identification and In Vitro Antifungal Susceptibility of Paecilomyces/Purpureocillium Species Isolated from Clinical Respiratory Samples: A Multicenter Study. Journal of Fungi. 2022; 8(7):684. https://doi.org/10.3390/jof8070684
Chicago/Turabian StyleMonpierre, Lorra, Nawel Aït-Ammar, Isabel Valsecchi, Anne-Cécile Normand, Juliette Guitard, Arnaud Riat, Antoine Huguenin, Christine Bonnal, Boualem Sendid, Lilia Hasseine, and et al. 2022. "Species Identification and In Vitro Antifungal Susceptibility of Paecilomyces/Purpureocillium Species Isolated from Clinical Respiratory Samples: A Multicenter Study" Journal of Fungi 8, no. 7: 684. https://doi.org/10.3390/jof8070684