Novel Insights into Aspergillus fumigatus Pathogenesis and Host Response from State-of-the-Art Imaging of Host–Pathogen Interactions during Infection
Abstract
:1. Introduction
2. Are A. fumigatus Spores Deposited in the Alveoli?
3. What Microenvironments Does A. fumigatus Encounter upon Lung Deposition?
4. Are Airway Respiratory Cells Internalising A. fumigatus during Infection?
5. How Does Germination Alter the Interactions of A. fumigatus with Immune Cells?
6. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Light Sheet Fluorescence Microscopy (LSFM) | Fluorescence Tomography/Bioluminescence Tomography | Imaging Flow Cytometry (IFC) | Zebrafish Model of Aspergillosis | |
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Key Study | Amich et al. (2020) [15] | Gresnigt et al. (2016) [16] | Bertuzzi et al. (2022) [17] | Rosowski et al. (2018) [18] |
Alternatives | Histology: labour intensive and practically unfeasible for whole lungs, potentially resulting in sampling bias;Confocal laser-scanning microscopy: requires same clearing process as LSFM and has similar disadvantages but may provide higher spatial resolution; however, the imaging depth is limited to approx. 1mm fluorescence signal-pending;ImmunoPET/MRI: similar to fluorescence tomography but primarily being explored as a diagnostic tool. |
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Ortiz, S.C.; Pennington, K.; Thomson, D.D.; Bertuzzi, M. Novel Insights into Aspergillus fumigatus Pathogenesis and Host Response from State-of-the-Art Imaging of Host–Pathogen Interactions during Infection. J. Fungi 2022, 8, 264. https://doi.org/10.3390/jof8030264
Ortiz SC, Pennington K, Thomson DD, Bertuzzi M. Novel Insights into Aspergillus fumigatus Pathogenesis and Host Response from State-of-the-Art Imaging of Host–Pathogen Interactions during Infection. Journal of Fungi. 2022; 8(3):264. https://doi.org/10.3390/jof8030264
Chicago/Turabian StyleOrtiz, Sébastien C., Katie Pennington, Darren D. Thomson, and Margherita Bertuzzi. 2022. "Novel Insights into Aspergillus fumigatus Pathogenesis and Host Response from State-of-the-Art Imaging of Host–Pathogen Interactions during Infection" Journal of Fungi 8, no. 3: 264. https://doi.org/10.3390/jof8030264