Identification of a C2H2 Transcription Factor (PsCZF3) Associated with RxLR Effectors and Carbohydrate-Active Enzymes in Phytophthora sojae Based on WGCNA
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strain and Culture Conditions
2.2. RNA Isolation, Library Preparation, and Sequencing
2.3. Digital RNA-Seq and Data Analysis
2.4. Weighted Gene Co-Expression Network Analysis
2.5. Gene Ontology (GO) Enrichment Analysis
2.6. DNA-Binding Site Prediction
2.7. Identification and Phylogenetic Analysis of PsCZF3 Orthologs
2.8. Validation by Reverse Transcription-Quantitative PCR (RT-qPCR)
3. Results
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hu, Y.; He, Z.; Kang, Y.; Ye, W.; Cui, L. Identification of a C2H2 Transcription Factor (PsCZF3) Associated with RxLR Effectors and Carbohydrate-Active Enzymes in Phytophthora sojae Based on WGCNA. J. Fungi 2022, 8, 998. https://doi.org/10.3390/jof8100998
Hu Y, He Z, Kang Y, Ye W, Cui L. Identification of a C2H2 Transcription Factor (PsCZF3) Associated with RxLR Effectors and Carbohydrate-Active Enzymes in Phytophthora sojae Based on WGCNA. Journal of Fungi. 2022; 8(10):998. https://doi.org/10.3390/jof8100998
Chicago/Turabian StyleHu, Yanhong, Zhihua He, Yebin Kang, Wenwu Ye, and Linkai Cui. 2022. "Identification of a C2H2 Transcription Factor (PsCZF3) Associated with RxLR Effectors and Carbohydrate-Active Enzymes in Phytophthora sojae Based on WGCNA" Journal of Fungi 8, no. 10: 998. https://doi.org/10.3390/jof8100998