Bioprospecting White-Rot Basidiomycete Irpex lacteus for Improved Extraction of Lignocellulose-Degrading Enzymes and Their Further Application
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungus and Culture Conditions
2.2. Lignocellulosic Substrates
2.3. Enzyme Production
2.4. Enzyme Extraction
2.5. Enzymatic Hydrolysis
2.6. Sugar Analysis
3. Results and Discussion
3.1. Fungal Cultivation Conditions in Biomass Substrate
3.2. Enzyme Extraction Efficiency
3.3. Production of Fermentable Sugars
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Substrate | Incubation Time | ||
---|---|---|---|
24 h | 48 h | 72 h | |
Hay | 207.5 ± 7.2 | 215.3 ± 4.8 | 221.7 ± 8.0 |
Wood residue chips | 50.3 ± 4.3 | 52.4 ± 4.2 | 59.6 ± 6.2 |
Sawing residue chips | 24.8 ± 0.7 | 28.0 ± 1.2 | 33.4 ± 1.8 |
Barley straw | 81.9 ± 6.6 | 104.3 ± 7.9 | 112.3 ± 10.3 |
Green algae (dried) | 93.7 ± 13.2 | 110.2 ± 5.7 | 113.6 ± 3.9 |
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Mezule, L.; Civzele, A. Bioprospecting White-Rot Basidiomycete Irpex lacteus for Improved Extraction of Lignocellulose-Degrading Enzymes and Their Further Application. J. Fungi 2020, 6, 256. https://doi.org/10.3390/jof6040256
Mezule L, Civzele A. Bioprospecting White-Rot Basidiomycete Irpex lacteus for Improved Extraction of Lignocellulose-Degrading Enzymes and Their Further Application. Journal of Fungi. 2020; 6(4):256. https://doi.org/10.3390/jof6040256
Chicago/Turabian StyleMezule, Linda, and Anna Civzele. 2020. "Bioprospecting White-Rot Basidiomycete Irpex lacteus for Improved Extraction of Lignocellulose-Degrading Enzymes and Their Further Application" Journal of Fungi 6, no. 4: 256. https://doi.org/10.3390/jof6040256
APA StyleMezule, L., & Civzele, A. (2020). Bioprospecting White-Rot Basidiomycete Irpex lacteus for Improved Extraction of Lignocellulose-Degrading Enzymes and Their Further Application. Journal of Fungi, 6(4), 256. https://doi.org/10.3390/jof6040256