Next Article in Journal
Invasive Mold Infection of the Central Nervous System in Immunocompromised Children
Previous Article in Journal
Use of the Versatility of Fungal Metabolism to Meet Modern Demands for Healthy Aging, Functional Foods, and Sustainability
Previous Article in Special Issue
Evaluation of Microsatellite Typing, ITS Sequencing, AFLP Fingerprinting, MALDI-TOF MS, and Fourier-Transform Infrared Spectroscopy Analysis of Candida auris
Open AccessArticle

Candida auris Direct Detection from Surveillance Swabs, Blood, and Urine Using a Laboratory-Developed PCR Method

Division of Clinical Microbiology, Mayo Clinic, Rochester, MN 55902, USA
Research and Innovation Office, Mayo Clinic, Rochester, MN 55902, USA
Infection Prevention and Control, Mayo Clinic, Rochester, MN 55902, USA
Public Health Laboratory, Minnesota Department of Health, St. Paul, MN 55164, USA
Author to whom correspondence should be addressed.
J. Fungi 2020, 6(4), 224;
Received: 8 September 2020 / Revised: 9 October 2020 / Accepted: 10 October 2020 / Published: 15 October 2020
(This article belongs to the Special Issue Candida auris 2.0)
Candida auris is an emerging fungal pathogen with cases reported in countries around the world and in 19 states within the United States as of August 2020. The CDC has recommended that hospitals perform active surveillance upon admission for patients with the appropriate risk factors. Currently, active surveillance requires that local hospitals send surveillance swabs to a public health laboratory for analysis. In this work, a real-time PCR assay was developed for the specific detection of C. auris from surveillance swabs, blood, and urine to enable rapid detection of this pathogen. The assay uses commercially available primers and reporter probes and it was verified on the LightCycler 480 PCR platform. Contrived specimens and prospectively collected composite groin/axilla surveillance swabs were used to validate the assay. The performance of the PCR assay on surveillance swabs was also compared to a second PCR assay targeting C. auris that was performed at the Minnesota Department of Health–Public Health Laboratory (MDH-PHL). Our PCR assay is able to detect and differentiate C. auris from closely related Candida species such as C. duobushaemulonii, C. haemulonii, and C. pseudohaemulonii on the basis of melting curve temperature differences. View Full-Text
Keywords: Candida auris; PCR; surveillance; yeast; identification Candida auris; PCR; surveillance; yeast; identification
Show Figures

Figure 1

MDPI and ACS Style

Walchak, R.C.; Buckwalter, S.P.; Zinsmaster, N.M.; Henn, K.M.; Johnson, K.M.; Koelsch, J.M.; Herring, S.A.; Steinmetz, L.K.; Reed, K.A.; Barth, J.E.; Rasmusson, J.M.; Fischer, J.L.; Snippes Vagnone, P.; Sampathkumar, P.; Wengenack, N.L. Candida auris Direct Detection from Surveillance Swabs, Blood, and Urine Using a Laboratory-Developed PCR Method. J. Fungi 2020, 6, 224.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop