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Inactivation of Dermatophytes Causing Onychomycosis and Its Therapy Using Non-Thermal Plasma

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Department of Epidemiology and Public Health, Faculty of Medicine, University of Ostrava, Syllabova 19, 703 00 Ostrava, Czech Republic
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Podiatric Center Medicia, Daliborova 421/15, 709 00 Ostrava, Czech Republic
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Department of Bacteriology and Mycology, Public Health Institute in Ostrava, Partyzánské nám. 7, 702 00 Ostrava, Czech Republic
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Department of Biomedical Sciences, Institute of Microbiology and Immunology, Faculty of Medicine, University of Ostrava, Syllabova 19, 703 00 Ostrava, Czech Republic
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Department of Dermatology and Venereology, First Faculty of Medicine, Charles University and General University Hospital in Prague, U Nemocnice 499/2, 128 08 Praha 2, Czech Republic
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Department of Dermatology, University Hospital Ostrava, 17. listopadu 1790/5, 708 52 Ostrava, Czech Republic
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Department of Physics and Measurements, Faculty of Chemical Engineering, University of Chemistry and Technology Prague, Technická 5, 166 28 Praha, Czech Republic
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Department of Computing and Control Engineering, Faculty of Chemical Engineering, University of Chemistry and Technology Prague, Technická 5, 166 28 Praha, Czech Republic
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Department of Mathematics, University of Chemistry and Technology Prague, Technická 5, 166 28 Praha, Czech Republic
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Department of Microbiology, University Hospital Olomouc, Hněvotínská 3, 775 15 Olomouc, Czech Republic
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Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University, Studničkova 7, 128 00 Praha 2, Czech Republic
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Author to whom correspondence should be addressed.
J. Fungi 2020, 6(4), 214; https://doi.org/10.3390/jof6040214
Received: 1 September 2020 / Revised: 18 September 2020 / Accepted: 1 October 2020 / Published: 10 October 2020
Onychomycosis is one of the most common nail disorders. Its current treatment is not satisfactorily effective and often causes adverse side effects. This study aims to determine the optimal conditions for non-thermal plasma (NTP) inactivation of the most common dermatophytes in vitro and to apply it in patient`s therapy. The in vitro exposure to NTP produced by negative DC corona discharge caused full inactivation of Trichophyton spp. if applied during the early growth phases. This effect decreased to negligible inactivation with the exposure applied six days after inoculation. In a group of 40 patients with onychomycosis, NTP therapy was combined with nail plate abrasion and refreshment (NPAR) or treatment with antimycotics. The cohort included 17 patients treated with NPAR combined with NTP, 11 patients treated with antimycotics and NTP, and 12 patients treated with NPAR alone. The combination of NPAR and NTP resulted in clinical cure in more than 70% of patients. The synergistic effect of NPAR and NTP caused 85.7% improvement of mycological cure confirmed by negative microscopy and culture of the affected nail plate. We conclude that NTP can significantly improve the treatment of onychomycosis. View Full-Text
Keywords: non-thermal plasma; Trichophyton; fungal inactivation; onychomycosis therapy non-thermal plasma; Trichophyton; fungal inactivation; onychomycosis therapy
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Lux, J.; Dobiáš, R.; Kuklová, I.; Litvik, R.; Scholtz, V.; Soušková, H.; Khun, J.; Mrázek, J.; Kantorová, M.; Jaworská, P.; Prejdová, T.; Šnupárková, J.; Hamal, P.; Julák, J. Inactivation of Dermatophytes Causing Onychomycosis and Its Therapy Using Non-Thermal Plasma. J. Fungi 2020, 6, 214.

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