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Open AccessArticle

Detection of Fusarium Species in Clinical Specimens by Probe-Based Real-Time PCR

1
Department for Internal Medicine II, University Hospital of Wuerzburg, 97080 Wuerzburg, Germany
2
National Reference Center for Invasive Fungal Infections (NRZMyk), Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, 07745 Jena, Germany
3
Robert Koch Institute Berlin, 13353 Berlin, Germany
4
Institute for Medical Microbiology, Immunology and Hygiene, University Hospital of Cologne, University of Cologne, 50935 Cologne, Germany
5
Institute for Medical Microbiology and Virology, Carl von Ossietzky University of Oldenburg, 26129 Oldenburg, Germany
6
Division of Clinical Microbiology, Institute of Hygiene and Medical Microbiology of the University, Vienna University Hospital, 1090 Vienna, Austria
7
Department of Hematology, Medical Oncology, and Pneumology, University Medical Center of the Johannes Gutenberg University, 55122 Mainz, Germany
8
Institute for Hygiene and Microbiology, University Wuerzburg, 97080 Wuerzburg, Germany
*
Author to whom correspondence should be addressed.
J. Fungi 2019, 5(4), 105; https://doi.org/10.3390/jof5040105
Received: 27 September 2019 / Revised: 6 November 2019 / Accepted: 7 November 2019 / Published: 12 November 2019
(This article belongs to the Special Issue Molecular Diagnostics of Fungal Infections)
The mold Fusarium is a ubiquitous fungus causing plant, animal and human infections. In humans, Fusarium spp. are the major cause of eye infections in patients wearing contact lenses or after local trauma. Systemic infections by Fusarium spp. mainly occur in immunosuppressed patients and can disseminate throughout the human body. Due to high levels of resistance to antifungals a fast identification of the causative agent is an urgent need. By using a probe-based real-time PCR assay specific for the genus Fusarium we analysed several different clinical specimens detecting Fusarium spp. commonly found in clinical samples in Germany. Also, a large collection of lung fluid samples of haematological patients was analysed (n = 243). In these, two samples (0.8%) were reproducibly positive, but only one could be confirmed by sequencing. For this case of probable invasive fungal disease (IFD) culture was positive for Fusarium species. Here we describe a rapid, probe-based real-time PCR assay to specifically detect DNA from a broad range of Fusarium species and its application to clinically relevant specimens. View Full-Text
Keywords: probe-based real-time PCR; Fusarium; bronchoalveolar lavage fluid; fungal molecular diagnostics probe-based real-time PCR; Fusarium; bronchoalveolar lavage fluid; fungal molecular diagnostics
MDPI and ACS Style

Springer, J.; Walther, G.; Rickerts, V.; Hamprecht, A.; Willinger, B.; Teschner, D.; Einsele, H.; Kurzai, O.; Loeffler, J. Detection of Fusarium Species in Clinical Specimens by Probe-Based Real-Time PCR. J. Fungi 2019, 5, 105.

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