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Molecular Diagnostics of Mucormycosis in Hematological Patients: A Literature Review

Department of Clinical Mycology, Allergy and Immunology, I. Mechnikov North Western State Medical University, Saint Petersburg 194291, Russia
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J. Fungi 2019, 5(4), 112; https://doi.org/10.3390/jof5040112
Received: 31 October 2019 / Revised: 26 November 2019 / Accepted: 27 November 2019 / Published: 29 November 2019
(This article belongs to the Special Issue Molecular Diagnostics of Fungal Infections)
Objectives: to analyze the results of molecular methods applying for the diagnosis of mucormycosis in hematologic patients based on a literature review. Data sources: A systematic search in databases PubMed, Google Scholar for August 2019. Review eligibility criteria: original articles published in English, studies of molecular methods for the diagnosis of mucormycosis in hematologic patients. Results. We analyzed the research data from 116 hematological patients with mucormycosis, including children (6%). Patients with localized forms of mucormycosis prevailed (72%), and lung involvement was diagnosed in 58% of these cases. For molecular verification of the causative agent of mucormycosis, blood serum was most often used, less commonly postoperative and autopsy material, biopsy specimens, formalin-fixed paraffin-embedded samples and bronchoalveolar lavage, pleural fluid and sputum. The sensitivity of molecular diagnostics of mucormycosis in a cohort of hematological patients was 88.2%. Conclusion. The use of molecular techniques along with standard mycological methods will improve the diagnostics of mucormycosis in hematologic patients. However, prospective studies of the effectiveness of molecular methods for the diagnosis of mucormycosis of various etiologies in hematological patients, including children, using bronchoalveolar lavage (BAL) and cerebrospinal fluid (CSF) are needed. View Full-Text
Keywords: hematological patients; Mucorales; mucormycosis; molecular diagnosis; PCR; real-time quantitative PCR; sequencing hematological patients; Mucorales; mucormycosis; molecular diagnosis; PCR; real-time quantitative PCR; sequencing
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Shadrivova, O.V.; Burygina, E.V.; Klimko, N.N. Molecular Diagnostics of Mucormycosis in Hematological Patients: A Literature Review. J. Fungi 2019, 5, 112.

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