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Open AccessArticle

Differences in Sirtuin Regulation in Response to Calorie Restriction in Cryptococcus neoformans

Department of Biomedical Sciences, Long Island University-Post, Brookville, NY 11548, USA
Department of Medicine (Division of Infectious Diseases) and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794 USA
Author to whom correspondence should be addressed.
J. Fungi 2018, 4(1), 26;
Received: 26 January 2018 / Revised: 15 February 2018 / Accepted: 16 February 2018 / Published: 18 February 2018
(This article belongs to the Special Issue Cryptococcus and Cryptococcosis)
Cryptococcus neoformans successfully replicates in low glucose in infected patients. In the serotype A strain, H99, growth in this condition prolongs lifespan regulated by SIR2, and can be modulated with SIR2-specific drugs. Previous studies show that lifespan modulation of a cryptococcal population affects its sensitivity to antifungals, and survival in an infection model. Sirtuins and their role in longevity are conserved among fungi; however, the effect of glucose starvation is not confirmed even in Saccharomyces cerevisiae. Lifespan analysis of C. neoformans strains in low glucose showed that 37.5% exhibited pro-longevity, and lifespan of a serotype D strain, RC2, was shortened. Transcriptome comparison of H99 and RC2 under calorie restriction demonstrated differences, confirmed by real-time PCR showing that SIR2, TOR1, SCH9, and PKA1 expression correlated with lifespan response to calorie restriction. As expected, RC2-sir2Δ cells exhibited a shortened lifespan, which was reconstituted. However, shortened lifespan from calorie restriction was independent of SIR2. In contrast to H99 but consistent with altered SIR2 regulation, SIR2-specific drugs did not affect outcome of RC2 infection. These data suggest that SIR2 regulation and response to calorie restriction varies in C. neoformans, which should be considered when Sirtuins are investigated as potential therapy targets for fungal infections. View Full-Text
Keywords: fungus; pathogen; sirtuins; aging; resilience; glucose fungus; pathogen; sirtuins; aging; resilience; glucose
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Bouklas, T.; Masone, L.; Fries, B.C. Differences in Sirtuin Regulation in Response to Calorie Restriction in Cryptococcus neoformans. J. Fungi 2018, 4, 26.

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