Utilization of High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry for Characterization of 8-O-methylbostrycoidin Production by Species of the Fungus Fusarium
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Fungal Strains and Preparation of Maize Materia
2.3. Extraction of 8-O-methylbostrycoidin from Maize
2.4. ESI-MS and MS/MS
2.5. HPLC-MS/MS
2.6. Spike/Recovery Experiments and Method Validation
2.7. Field Test
3. Results
3.1. MS and MS/MS by Flow Injection
3.2. HPLC Coupled to MS
3.3. LC-MS/MS Quantitation
3.4. Spike Recovery Studies
3.5. Matrix Interference
3.6. Determination of 8-O-methylbostrycoidin from Field Grown Maize
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Strain | Disease Score 1 | Level of 8-O-methylbostrycoidin (µg/g) 1 |
---|---|---|
AMR 5 | 4.9 (1.9) | 0.5 (0.9) |
AMR 10 | 4.4 (1.6) | 2.6 (4.8) |
Control inoculation | 1.3 (0.9) | 0 (–) |
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Busman, M. Utilization of High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry for Characterization of 8-O-methylbostrycoidin Production by Species of the Fungus Fusarium. J. Fungi 2017, 3, 43. https://doi.org/10.3390/jof3030043
Busman M. Utilization of High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry for Characterization of 8-O-methylbostrycoidin Production by Species of the Fungus Fusarium. Journal of Fungi. 2017; 3(3):43. https://doi.org/10.3390/jof3030043
Chicago/Turabian StyleBusman, Mark. 2017. "Utilization of High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry for Characterization of 8-O-methylbostrycoidin Production by Species of the Fungus Fusarium" Journal of Fungi 3, no. 3: 43. https://doi.org/10.3390/jof3030043