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Open AccessArticle

The Effect of Novel Heterocyclic Compounds on Cryptococcal Biofilm

Department of Clinical Microbiology and Infectious Diseases, Hadassah-Hebrew University Medical Center, Jerusalem 91120, Israel
Author to whom correspondence should be addressed.
Current address: Department of Medical Neurobiology, Institute for Medical Research Israel-Canada, Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
J. Fungi 2017, 3(3), 42;
Received: 29 May 2017 / Revised: 13 July 2017 / Accepted: 17 July 2017 / Published: 20 July 2017
(This article belongs to the Special Issue Fungal Biofilms)
Biofilm formation by microorganisms depends on their communication by quorum sensing, which is mediated by small diffusible signaling molecules that accumulate in the extracellular environment. During human infection, the pathogenic yeast Cryptococcus neoformans can form biofilm on medical devices, which protects the organism and increases its resistance to antifungal agents. The aim of this study was to test two novel heterocyclic compounds, S-8 (thiazolidinedione derivative, TZD) and NA-8 (succinimide derivative, SI), for their anti-biofilm activity against strains of Cryptococcus neoformans and Cryptococcus gattii. Biofilms were formed in a defined medium in 96-well polystyrene plates and 8-well micro-slides. The effect of sub-inhibitory concentrations of S-8 and NA-8 on biofilm formation was measured after 48 h by a metabolic reduction assay and by confocal laser microscopy analysis using fluorescent staining. The formation and development of cryptococcal biofilms was inhibited significantly by these compounds in concentrations below the minimum inhibitory concentration (MIC) values. These compounds may have a potential role in preventing fungal biofilm development on indwelling medical devices or even as a therapeutic measure after the establishment of biofilm. View Full-Text
Keywords: Cryptococcus; biofilm; quorum sensing; thiazolidinedione derivative; succinimide derivative; auto-inducer Cryptococcus; biofilm; quorum sensing; thiazolidinedione derivative; succinimide derivative; auto-inducer
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MDPI and ACS Style

Korem, M.; Kagan, S.; Polacheck, I. The Effect of Novel Heterocyclic Compounds on Cryptococcal Biofilm. J. Fungi 2017, 3, 42.

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