Utilization of High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry for Characterization of 8-O-methylbostrycoidin Production by Species of the Fungus Fusarium
by
Mark Busman
Mark Busman
Mycotoxin Prevention and Applied Microbiology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, IL 61604, USA
J. Fungi 2017, 3(3), 43; https://doi.org/10.3390/jof3030043
Received: 1 June 2017 / Revised: 14 July 2017 / Accepted: 20 July 2017 / Published: 25 July 2017
(This article belongs to the Special Issue Fungal Pigments)
The pigment 8-O-methylbostrycoidin is a polyketide metabolite produced by multiple species of the fungus Fusarium that infects plant crops, including maize. A technique was developed for the analysis of 8-O-methylbostrycoidin by high performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry. The quantitative nature of the LC-MS/MS experiment was demonstrated over a range of concentrations in maize. Limits of detection for the method (10 ng/g from 8-O-methylbostrycoidin spiked into ground maize) were shown, and susceptibility of the method to matrix effects from maize was also evaluated. The method was applied to evaluate the ability of the maize pathogen Fusarium verticillioides to produce 8-O-methylbostrycoidin in developing maize ears grown in an agricultural field.
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MDPI and ACS Style
Busman, M. Utilization of High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry for Characterization of 8-O-methylbostrycoidin Production by Species of the Fungus Fusarium. J. Fungi 2017, 3, 43.
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