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Brief Report

Poly ADP-Ribosylation in a Plant Pathogenic Oomycete Phytophthora infestans: A Key Controller of Growth and Host Plant Colonisation

by
Viktoriya O. Samarskaya
1,
Sofya Koblova
2,
Tatiana Suprunova
2,
Eugene A. Rogozhin
1,
Nadezhda Spechenkova
1,
Sofiya Yakunina
1,
Andrew J. Love
3,
Natalia O. Kalinina
4 and
Michael Taliansky
1,3,*
1
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia
2
Doka-Gene Technologies Ltd., Moscow Region, 141880 Rogachevo, Russia
3
James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK
4
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia
*
Author to whom correspondence should be addressed.
J. Fungi 2025, 11(1), 29; https://doi.org/10.3390/jof11010029
Submission received: 6 November 2024 / Revised: 27 December 2024 / Accepted: 31 December 2024 / Published: 3 January 2025
(This article belongs to the Special Issue Current Research in Soil Borne Plant Pathogens)
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Abstract

ADP-ribosylation is a reversible modification of proteins and nucleic acids, which controls major cellular processes, including DNA damage repair, cell proliferation and differentiation, metabolism, stress, and immunity in plants and animals. The involvement of ADP-ribosylation in the life cycle of Dictyostelium and some filamentous fungi has also been demonstrated. However, the role of this process in pathogenic oomycetes has never been addressed. Here, we show that the Phytophthora infestans genome contains two PARP-like protein genes (PiPARP1 and PiPARP2), and provide evidence of PARylation activity for one of them (PiPARP2). Using dsRNA-mediated RNA silencing of the PiPARP2 gene and chemical (pharmacological) inhibition of PARP activity by 3-aminobenzamide (3AB) PARP inhibitor, we demonstrate the critical functional role of ADP-ribosylation in Phytophthora mycelium growth. Virulence test on detached leaves also suggests an important role of ADP-ribosylation in Phytophthora host plant colonisation and pathogenesis. On a practical level, our data suggest that targeting the PARylation system may constitute a novel powerful approach for the management of Phytophthora diseases.
Keywords: Phytophthora infestans; potato; RNA interference; dsRNA; poly ADP-ribosylation; PARP inhibitor Phytophthora infestans; potato; RNA interference; dsRNA; poly ADP-ribosylation; PARP inhibitor

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MDPI and ACS Style

Samarskaya, V.O.; Koblova, S.; Suprunova, T.; Rogozhin, E.A.; Spechenkova, N.; Yakunina, S.; Love, A.J.; Kalinina, N.O.; Taliansky, M. Poly ADP-Ribosylation in a Plant Pathogenic Oomycete Phytophthora infestans: A Key Controller of Growth and Host Plant Colonisation. J. Fungi 2025, 11, 29. https://doi.org/10.3390/jof11010029

AMA Style

Samarskaya VO, Koblova S, Suprunova T, Rogozhin EA, Spechenkova N, Yakunina S, Love AJ, Kalinina NO, Taliansky M. Poly ADP-Ribosylation in a Plant Pathogenic Oomycete Phytophthora infestans: A Key Controller of Growth and Host Plant Colonisation. Journal of Fungi. 2025; 11(1):29. https://doi.org/10.3390/jof11010029

Chicago/Turabian Style

Samarskaya, Viktoriya O., Sofya Koblova, Tatiana Suprunova, Eugene A. Rogozhin, Nadezhda Spechenkova, Sofiya Yakunina, Andrew J. Love, Natalia O. Kalinina, and Michael Taliansky. 2025. "Poly ADP-Ribosylation in a Plant Pathogenic Oomycete Phytophthora infestans: A Key Controller of Growth and Host Plant Colonisation" Journal of Fungi 11, no. 1: 29. https://doi.org/10.3390/jof11010029

APA Style

Samarskaya, V. O., Koblova, S., Suprunova, T., Rogozhin, E. A., Spechenkova, N., Yakunina, S., Love, A. J., Kalinina, N. O., & Taliansky, M. (2025). Poly ADP-Ribosylation in a Plant Pathogenic Oomycete Phytophthora infestans: A Key Controller of Growth and Host Plant Colonisation. Journal of Fungi, 11(1), 29. https://doi.org/10.3390/jof11010029

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