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Communication

Rapid Molecular Diagnosis of Sporotrichosis Directly from Biological Samples from a Reference Center in Brazil

by
Amanda Gabriela da Silva
1,2,
Arthur Felipe Cavalcanti de Matos
2,
Bruna Rodrigues de Sousa
1,2,
Claudia Elise Ferraz
3,
Raul Leal Faria Luiz
4,
Rejane Pereira Neves
1,5,
Reginaldo Gonçalves de Lima-Neto
1,2,3,5,*,† and
Manoel Marques Evangelista Oliveira
4,†
1
Postgraduate Program in Tropical Medicine, Center for Medical Sciences, Federal University of Pernambuco (UFPE), Recife 50740-570, Pernambuco, Brazil
2
Laboratory for Research and Diagnosis in Tropical Diseases, Federal University of Pernambuco (UFPE), Recife 50740-570, Pernambuco, Brazil
3
Dermatology Reference Service, Hospital das Clínicas, Federal University of Pernambuco (UFPE), Recife 50670-901, Pernambuco, Brazil
4
Oswaldo Cruz Institute, FIOCRUZ, Av Brasil, 4365, Manguinhos, Rio de Janeiro CEP 21045-360, Brazil
5
Postgraduate Program in Fungal Biology, Biosciences Center, Federal University of Pernambuco (UFPE), Recife 50740-570, Pernambuco, Brazil
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
J. Fungi 2024, 10(6), 432; https://doi.org/10.3390/jof10060432
Submission received: 30 April 2024 / Revised: 13 June 2024 / Accepted: 14 June 2024 / Published: 18 June 2024
(This article belongs to the Section Fungal Pathogenesis and Disease Control)
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Abstract

The gold standard diagnosis of sporotrichosis is the isolation of Sporothrix sp. in culture media, but this is a time-consuming test that is susceptible to contamination and can be affected by the fungal load. Molecular methods such as nested PCR are gaining more ground in the management of several infections as they are tools for the rapid and accurate identification of microorganisms from pure cultures or directly from biological samples. This study aimed to apply a nested PCR molecular protocol for the rapid detection of Sporothrix spp. directly from clinical samples. Thirteen samples—six from skin biopsies, five from skin exudates, and two from conjunctival secretions—were obtained from patients diagnosed with sporotrichosis due to S. brasiliensis. Calmodulin gene sequencing identified all the isolates as S. brasiliensis. Nested PCR was able to detect all the Sporothrix sensu lato directly from clinical samples as well as the CBS 120339 reference strain. The nested PCR protocol stands out as a diagnostic alternative, as it allows the identification of Sporothrix spp. directly from clinical samples without the need for fungal isolation.
Keywords: Sporothrix; nested PCR; skin biopsy; skin exudate; ocular secretion Sporothrix; nested PCR; skin biopsy; skin exudate; ocular secretion

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MDPI and ACS Style

da Silva, A.G.; de Matos, A.F.C.; de Sousa, B.R.; Ferraz, C.E.; Luiz, R.L.F.; Neves, R.P.; Lima-Neto, R.G.d.; Oliveira, M.M.E. Rapid Molecular Diagnosis of Sporotrichosis Directly from Biological Samples from a Reference Center in Brazil. J. Fungi 2024, 10, 432. https://doi.org/10.3390/jof10060432

AMA Style

da Silva AG, de Matos AFC, de Sousa BR, Ferraz CE, Luiz RLF, Neves RP, Lima-Neto RGd, Oliveira MME. Rapid Molecular Diagnosis of Sporotrichosis Directly from Biological Samples from a Reference Center in Brazil. Journal of Fungi. 2024; 10(6):432. https://doi.org/10.3390/jof10060432

Chicago/Turabian Style

da Silva, Amanda Gabriela, Arthur Felipe Cavalcanti de Matos, Bruna Rodrigues de Sousa, Claudia Elise Ferraz, Raul Leal Faria Luiz, Rejane Pereira Neves, Reginaldo Gonçalves de Lima-Neto, and Manoel Marques Evangelista Oliveira. 2024. "Rapid Molecular Diagnosis of Sporotrichosis Directly from Biological Samples from a Reference Center in Brazil" Journal of Fungi 10, no. 6: 432. https://doi.org/10.3390/jof10060432

APA Style

da Silva, A. G., de Matos, A. F. C., de Sousa, B. R., Ferraz, C. E., Luiz, R. L. F., Neves, R. P., Lima-Neto, R. G. d., & Oliveira, M. M. E. (2024). Rapid Molecular Diagnosis of Sporotrichosis Directly from Biological Samples from a Reference Center in Brazil. Journal of Fungi, 10(6), 432. https://doi.org/10.3390/jof10060432

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