Identification and Characterization of Eimeria tenella Rhoptry Protein 35 (EtROP35)
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Parasites and Animals
2.2. Molecular Cloning and Bioinformatic Analysis of EtROP35
2.3. Localization of EtROP35
2.4. Sporozoite Secretion Experiment
2.5. The Transcription and Expression Levels of EtROP35 in Different Developmental Stages of E. tenella
2.6. E. tenella Sporozoite Invasion-Blocking Assay
2.7. The Immune Protection of EtROP35 Proteins
2.8. Detection of Serum Antibody Level
2.9. Statistical Analysis
3. Results
3.1. Identification of EtROP35
3.2. The Localization of EtROP35
3.3. Expression of EtROP35 during Different Developmental Stages of E. tenella
3.4. Effect of EtROP35 on E. tenella Sporozoite Invasion of Host Cells
3.5. Immune Protection Induced against E. tenella by Recombinant EtROP35
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, B.; Zhao, N.; Sun, J.; Sun, L.; Li, H.; Wu, Z.; Li, H.; Zhang, X.; Zhao, X. Identification and Characterization of Eimeria tenella Rhoptry Protein 35 (EtROP35). Vet. Sci. 2022, 9, 465. https://doi.org/10.3390/vetsci9090465
Wang B, Zhao N, Sun J, Sun L, Li H, Wu Z, Li H, Zhang X, Zhao X. Identification and Characterization of Eimeria tenella Rhoptry Protein 35 (EtROP35). Veterinary Sciences. 2022; 9(9):465. https://doi.org/10.3390/vetsci9090465
Chicago/Turabian StyleWang, Bingxiang, Ningning Zhao, Jinkun Sun, Lingyu Sun, Huihui Li, Zhiyuan Wu, Hongmei Li, Xiao Zhang, and Xiaomin Zhao. 2022. "Identification and Characterization of Eimeria tenella Rhoptry Protein 35 (EtROP35)" Veterinary Sciences 9, no. 9: 465. https://doi.org/10.3390/vetsci9090465