Comparison of Two Leptospira Type Strains of Serovar Grippotyphosa in Microscopic Agglutination Test (MAT) Diagnostics for the Detection of Infections with Leptospires in Horses, Dogs and Pigs
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Samples
2.2. MAT
2.3. Detection of Pathogenic Leptospira by PCR
2.4. Statistical Analyses
3. Results and Discussion
3.1. Differences in MAT Titres with Moskva and Duyster
3.2. MAT Titres by Animal Species and Sample Material by Grippotyphosa Type Strains Only
3.3. Assessment of Discrepant MAT Results with the Serovar Grippotyphosa Type Strains Considering Further Information and Supplementary Test Results
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Serogroup | Positive Samples per Serogroup * | |||||
---|---|---|---|---|---|---|
Pig | Dog | Horse | ||||
Number | Percentage | Number | Percentage | Number | Percentage | |
Australis | 437 | 14.6 | 9 | 16.4 | 14 | 12.2 |
Autumnalis | 127 | 4.2 | 0 | 0.0 | 3 | 2.6 |
Canicola | 11 | 0.4 | 0 | 0.0 | 1 | 0.9 |
Grippotyphosa | 19 | 0.6 | 11 | 20.0 | 41 | 35.7 |
Ictero-haemorrhagiae | 274 | 9.1 | 15 | 27.3 | 5 | 4.3 |
Pomona | 42 | 1.4 | 3 | 5.5 | 4 | 3.5 |
Sejroe | 13 | 0.4 | 2 | 3.6 | 3 | 2.6 |
Tarassovi | 67 | 2.2 | 0 | 0.0 | 1 | 0.9 |
Multiple | 185 | 6.2 | 10 | 18.2 | 9 | 7.8 |
Negative | 1821 | 60.8 | 5 | 9.1 | 34 | 29.6 |
Total | 2996 | 100.0 | 55 | 100.0 | 115 | 100.0 |
Titre (a) | <25 * | 25 | 50 | 100 | 200 | 400 | 800 | 1600 | 3200 | >3200 * |
---|---|---|---|---|---|---|---|---|---|---|
Titre level (a) | 0 (negative) | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
Log2-titre (a) | −3.3 | 4.6 | 5.6 | 6.6 | 7.6 | 8.6 | 9.6 | 10.6 | 11.6 | 12.6 |
Titre (b) | <100 | 100 | 200 | 400 | 800 | 1600 | 3200 | >3200 | ||
Titre level (b) | 0 (negative) | 3 | 4 | 5 | 6 | 7 | 8 | 9 | ||
Log2-titre (b) | −3.3 | 6.6 | 7.6 | 8.6 | 9.6 | 10.6 | 11.6 | 12.6 |
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Strutzberg-Minder, K.; Ullerich, A.; Dohmann, K.; Boehmer, J.; Goris, M. Comparison of Two Leptospira Type Strains of Serovar Grippotyphosa in Microscopic Agglutination Test (MAT) Diagnostics for the Detection of Infections with Leptospires in Horses, Dogs and Pigs. Vet. Sci. 2022, 9, 464. https://doi.org/10.3390/vetsci9090464
Strutzberg-Minder K, Ullerich A, Dohmann K, Boehmer J, Goris M. Comparison of Two Leptospira Type Strains of Serovar Grippotyphosa in Microscopic Agglutination Test (MAT) Diagnostics for the Detection of Infections with Leptospires in Horses, Dogs and Pigs. Veterinary Sciences. 2022; 9(9):464. https://doi.org/10.3390/vetsci9090464
Chicago/Turabian StyleStrutzberg-Minder, Katrin, Astrid Ullerich, Karen Dohmann, Jan Boehmer, and Marga Goris. 2022. "Comparison of Two Leptospira Type Strains of Serovar Grippotyphosa in Microscopic Agglutination Test (MAT) Diagnostics for the Detection of Infections with Leptospires in Horses, Dogs and Pigs" Veterinary Sciences 9, no. 9: 464. https://doi.org/10.3390/vetsci9090464