Heparin and Progesterone Exert Synergistic Effects to Improve the In-Vitro Fertilization Rate of Bovine Sperm Bound to Oviduct Cell Aggregates from the Isthmus
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Experiment 1: Effect of Pre-Incubation of Sperm with Cell Aggregates from Different Oviduct Segments on In-Vitro Fertilization Rate
2.2. Experiment 2: Impact of the Addition of Mature COCs Treated with Heparin or Progesterone to Sperm Pre-Incubated with Isthmus Cells Aggregates on In-Vitro Fertilization Outcomes
2.3. Experiment 3: Influence of Addition of Mature COCs Treated with a Combination of Heparin and Progesterone to Sperm Pre-Incubated with Isthmus Cells Aggregates on In-Vitro Fertilization Rate
2.4. Collection of Oviduct Epithelial Cells
2.5. Sperm Processing and Preparation
2.6. Assay of Sperm Binding to Oviduct Epithelial Cells
2.7. Oocyte Preparation
2.8. In-Vitro Fertilization (IVF) and Culture (IVC)
2.9. Nuclear Staining of Embryos
2.10. Statistical Analysis
3. Results
3.1. Experiment 1: Effect of Pre-Incubation of Sperm with Cell Aggregates from Different Oviduct Segments on In-Vitro Fertilization Rate
3.2. Experiment 2: Impact of the Addition of Mature COCs Treated with Heparin or Progesterone to Sperm Pre-Incubated with Isthmus Cells Aggregates on In-Vitro Fertilization Outcomes
3.3. Experiment 3: Influence of Addition of Mature COCs Treated with a Combination of Heparin and Progesterone to Sperm Pre-Incubated with Isthmus Cells Aggregates on In-Vitro Fertilization Rate
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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El-Sokary, M.M.M.; Shehata, S.F.; Mahmoud, K.G.M. Heparin and Progesterone Exert Synergistic Effects to Improve the In-Vitro Fertilization Rate of Bovine Sperm Bound to Oviduct Cell Aggregates from the Isthmus. Vet. Sci. 2022, 9, 372. https://doi.org/10.3390/vetsci9070372
El-Sokary MMM, Shehata SF, Mahmoud KGM. Heparin and Progesterone Exert Synergistic Effects to Improve the In-Vitro Fertilization Rate of Bovine Sperm Bound to Oviduct Cell Aggregates from the Isthmus. Veterinary Sciences. 2022; 9(7):372. https://doi.org/10.3390/vetsci9070372
Chicago/Turabian StyleEl-Sokary, Mohamed M. M., Seham F. Shehata, and Karima G. M. Mahmoud. 2022. "Heparin and Progesterone Exert Synergistic Effects to Improve the In-Vitro Fertilization Rate of Bovine Sperm Bound to Oviduct Cell Aggregates from the Isthmus" Veterinary Sciences 9, no. 7: 372. https://doi.org/10.3390/vetsci9070372