Application of ORF3 Subunit Vaccine for Avian Hepatitis E Virus
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Chicks
2.2. Viruses and Plasmids
2.3. Primer Designs and Synthesis
2.4. Amplification of ORF3 Genes and Plasmids Construction
2.5. Protein-Induced Expression and Western Blot Identification
2.6. Vaccine Immunization
2.7. IFA Detections of HEV Antibodies
2.8. Artificial Challenge Test
3. Results
3.1. Construction and Homology Comparison of the ORF3 Recombinant Plasmids of YT-aHEV and VaHEV Strains
3.2. Induced Expression, Protein Purification and Western Blot Assay of Recombinant Bacteria
3.3. HEV Antibodies Detection by IFA
3.4. Results of the Challenge Experiment
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer Name | Primer Sequences (5′–3′) |
Va-ORF3-F | GGATCCatgCGCCTCGGCTGCCAGCAC (BamHI GGATCC) |
Va-ORF3-R | CTCGAGctaCATCTGGTACCGTGCG (XhoI CTCGAG) |
YT-ORF3-F | GGATCCatgTGTCTTAGTTGCCAGTT (BamHI GGATCC) |
YT-ORF3-R | CTCGAGctaCGTCTGGTACCGTGCGA (XhoI CTCGAG) |
Days After Challenge | VaHEV Protein Immunization Group | YT aHEV Protein Immunization Group | Mixed Immunization Group | Negative Control Group |
---|---|---|---|---|
2 | 5/8 | 5/8 | 3/8 | 8/8 |
4 | 1/8 | 0/8 | 0/8 | 8/8 |
7 | 0/8 | 0/8 | 0/8 | 8/8 |
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Yan, H.; Chi, Z.; Zhao, H.; Zhang, Y.; Zhang, Y.; Wang, Y.; Chang, S.; Zhao, P. Application of ORF3 Subunit Vaccine for Avian Hepatitis E Virus. Vet. Sci. 2022, 9, 676. https://doi.org/10.3390/vetsci9120676
Yan H, Chi Z, Zhao H, Zhang Y, Zhang Y, Wang Y, Chang S, Zhao P. Application of ORF3 Subunit Vaccine for Avian Hepatitis E Virus. Veterinary Sciences. 2022; 9(12):676. https://doi.org/10.3390/vetsci9120676
Chicago/Turabian StyleYan, Hongjian, Zengna Chi, Hui Zhao, Yawen Zhang, Yuduo Zhang, Yixin Wang, Shuang Chang, and Peng Zhao. 2022. "Application of ORF3 Subunit Vaccine for Avian Hepatitis E Virus" Veterinary Sciences 9, no. 12: 676. https://doi.org/10.3390/vetsci9120676