Transcriptome Analysis Reveals Genes Contributed to Min Pig Villi Hair Follicle in Different Seasons
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Phenotype Observation of Hair and Follicles of Min Pigs
2.3. Data Collection
2.4. Differentially Expressed Gene (DEG) Analysis
2.5. Gene Enrichment Analysis
2.6. Construction of an mRNA Interactive Network
2.7. Identification and Validation of mRNAs by qRT-PCR
2.8. Promoter Methylation Analysis of Core Genes
3. Results
3.1. The Hair Phenotype of Min Pigs
3.1.1. Observations of Hairs of Min Pigs
3.1.2. Villus Length and Density in Min Pigs
3.1.3. Tissue Analysis of Hair Follicles in Winter in Min Pigs
3.2. Identification of Differentially Expressed Genes (DEG) in the Skin of Pigs in Different Seasons
3.3. Functional Enrichment Analysis
3.4. Gene Enrichment Analysis
3.5. Identification and Validation of the Selected Genes
3.6. Transcription Factor Enrichment Analysis and Verification of PPI Network Genes
3.7. Methylation Analysis of Promoter of Target Genes
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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mRNA | Forward Primer | Reverse Primer |
---|---|---|
DSP | CGGGTACAATGACCCCGAAA | TTCCGGCCACGGACATCATC |
KRT15 | GATCGAGAGCCTGAACGAGG | GAGGCTTCTCTGGTGCCAAT |
KRT4 | ATCAGCTGACTCTTCACCGC | GCCTTCTCCCCAAGGAACAAA |
KRT85 | CATGAATGTGTTGTCCCGCA | GGTAGGTGGCGATCTCGATG |
TGM1 | TACAAGGAGTACCAGCCCCA | ACTGCCAGCCATGCTTCTTA |
DSG4 | GTGCGCACAATGTCCAGTTT | GCAGTCACACTCGGAAGACA |
DSC3 | GAGGGAGTTCCCACCTGTTG | AGCCCATCTTCTCTTGGCAC |
PKP1 | TGGCCTACGAATGCTTCCAG | GCGGTCCCGTAGTTGTTGTA |
transcription factor AP-1 | ACTTTCCTCCTTCACGGTCC | ACTGGATTATCAGGCGCTCG |
GAPDH | AGGTCGGTGTGAACGGATTTG | GGGGTCGTTGATGGCAACA |
Gene | Forward Primer | Reverse Primer | |
---|---|---|---|
DSP | M | GTAGTTGGATAAAATTAAAGTCGAT | TCAAATAATCCATCCTCTCGAA |
U | GGTAGTTGGATAAAATTAAAGTTG | TCAAATAATCCATCCTCTCAAAA | |
Probe | TTTCAACAAATTCTCATACTCCTCCTCCAA | ||
KRT15 | M | GGTTGGAGTAGGAGATCGTTA | CTAACTCCTCGACGTTAATACGAA |
U | GGTTGGAGTAGGAGATTGTTA | CTCCTCAACATTAATACAAACTTTACC | |
Probe | CCACCTCCCAAAAAAACTTCTCTAATACCA | ||
KRT4 | M | TAAAGGATGTTTATAGTAAGCGTG | ATCAACTCCTAATATTCACGCAA |
U | AAAGGATGTTTATAGTAAGTGTG | ATCAACTCCTAATATTCACACAA | |
Probe | AACCAACTCCTCCTTAACCTTCTTCAAAAC | ||
TGM1 | M | GAGGTTTAGAAGTTTTCGGA | CAATTAAACTCTAACAAACGACC |
U | GGAGGTTTAGAAGTTTTTGGAA | CAATTAAACTCTAACAAACAACC | |
Probe | CCATAAAACTTAAAATTCACCCTCAAACAA | ||
PKP1 | M | GGGATTAGGGTTGGTAGGAC | TCCGATAAAACACAATCCGA |
U | GGGATTAGGGTTGGTAGGATG | CCAATAAAACACAATCCAAAA | |
Probe | ACCAACCCCTCCTAACTCCTAAAACAAACC |
Hair Length (mm) | Yorkshire | Berkshire | Min Pig | |
Bristles | Bristles | Bristles | Villus | |
41.51 ± 4.7 | 49.48 ± 2.44 | 52.74 ± 2.05 | 30.55 ± 4.72 |
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Tian, M.; He, X.; Wang, W.; Feng, Y.; Zhang, D.; Li, Z.; Liu, D. Transcriptome Analysis Reveals Genes Contributed to Min Pig Villi Hair Follicle in Different Seasons. Vet. Sci. 2022, 9, 639. https://doi.org/10.3390/vetsci9110639
Tian M, He X, Wang W, Feng Y, Zhang D, Li Z, Liu D. Transcriptome Analysis Reveals Genes Contributed to Min Pig Villi Hair Follicle in Different Seasons. Veterinary Sciences. 2022; 9(11):639. https://doi.org/10.3390/vetsci9110639
Chicago/Turabian StyleTian, Ming, Xinmiao He, Wentao Wang, Yanzhong Feng, Dongjie Zhang, Zhongqiu Li, and Di Liu. 2022. "Transcriptome Analysis Reveals Genes Contributed to Min Pig Villi Hair Follicle in Different Seasons" Veterinary Sciences 9, no. 11: 639. https://doi.org/10.3390/vetsci9110639