In Vitro Assessment of Lyophilized Advanced Platelet-Rich Fibrin from Dogs in Promotion of Growth Factor Release and Wound Healing
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethical Approval, Animal Selection, and Study Design
2.2. Preparation of Canine Fresh A-PRF and Lyophilized A-PRF
2.3. Enzyme-Linked Immunosorbent Assay (ELISA) for the Measurement of Growth Factors
2.4. Evaluation of Biological Properties
2.4.1. Cell Culture
2.4.2. Proliferation Assay
2.4.3. Scratch Wound Assay
2.5. Statistical Analysis
3. Results
3.1. Growth Factor Release from the Fresh and Lyophilized Canine A-PRF
3.2. Influence of Fresh A-PRF and Lyophilized Canine A-PRF on Cell Proliferation
3.3. Influence of Fresh A-PRF and Lyophilized Canine A-PRF on Wound Closure
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Warin, R.; Vongchan, P.; Suriyasathaporn, W.; Boripun, R.; Suriyasathaporn, W. In Vitro Assessment of Lyophilized Advanced Platelet-Rich Fibrin from Dogs in Promotion of Growth Factor Release and Wound Healing. Vet. Sci. 2022, 9, 566. https://doi.org/10.3390/vetsci9100566
Warin R, Vongchan P, Suriyasathaporn W, Boripun R, Suriyasathaporn W. In Vitro Assessment of Lyophilized Advanced Platelet-Rich Fibrin from Dogs in Promotion of Growth Factor Release and Wound Healing. Veterinary Sciences. 2022; 9(10):566. https://doi.org/10.3390/vetsci9100566
Chicago/Turabian StyleWarin, Ravisa, Preeyanat Vongchan, Witaya Suriyasathaporn, Ratchadaporn Boripun, and Wanna Suriyasathaporn. 2022. "In Vitro Assessment of Lyophilized Advanced Platelet-Rich Fibrin from Dogs in Promotion of Growth Factor Release and Wound Healing" Veterinary Sciences 9, no. 10: 566. https://doi.org/10.3390/vetsci9100566