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Article

Effect of Cannabidiol (CBD) on Canine Inflammatory Response: An Ex Vivo Study on LPS Stimulated Whole Blood

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Department of Veterinary Science, University of Messina, 98166 Messina, Italy
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Department of Chemical, Biological, Pharmaceutical, and Environmental Science, University of Messina, 98166 Messina, Italy
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Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, 98166 Messina, Italy
4
Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, Saint Louis, MO 63104, USA
*
Authors to whom correspondence should be addressed.
These authors shared senior authorship.
Vet. Sci. 2021, 8(9), 185; https://doi.org/10.3390/vetsci8090185
Received: 13 July 2021 / Revised: 25 August 2021 / Accepted: 2 September 2021 / Published: 4 September 2021
(This article belongs to the Section Veterinary Microbiology, Parasitology and Immunology)
The use of cannabidiol (CBD) for animal species is an area of growing interest, for example for its anti-inflammatory and immuno-modulating properties, even though all of its biological effects are still not fully understood, especially in veterinary medicine. Therefore, the aim of this study was to investigate the anti-inflammatory and immuno-modulating properties of CBD for the first time directly in canine inflammatory response. We used an ex vivo model of LPS-stimulated whole dog blood. We stimulated the whole blood from healthy dogs with LPS 100 ng/mL for 24 h in the presence or not of CBD 50 and 100 μg/mL. We observed a reduction in IL-6 and TNF-α production from the group treated with CBD, but non-altered IL-10 levels. Moreover, we also observed from the CBD-treated group a reduction in Nf-κB and COX-2 expression. In conclusion, we demonstrated for the first time the anti-inflammatory and immuno-modulating properties of CBD directly in dogs’ immune cells, using a canine ex vivo inflammatory model. The results obtained from these studies encourage further studies to better understand the possible therapeutic role of CBD in veterinary medicine. View Full-Text
Keywords: cannabidiol; ex vivo; whole dog blood; canine inflammatory response cannabidiol; ex vivo; whole dog blood; canine inflammatory response
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MDPI and ACS Style

Gugliandolo, E.; Licata, P.; Peritore, A.F.; Siracusa, R.; D’Amico, R.; Cordaro, M.; Fusco, R.; Impellizzeri, D.; Di Paola, R.; Cuzzocrea, S.; Crupi, R.; Interlandi, C.D. Effect of Cannabidiol (CBD) on Canine Inflammatory Response: An Ex Vivo Study on LPS Stimulated Whole Blood. Vet. Sci. 2021, 8, 185. https://doi.org/10.3390/vetsci8090185

AMA Style

Gugliandolo E, Licata P, Peritore AF, Siracusa R, D’Amico R, Cordaro M, Fusco R, Impellizzeri D, Di Paola R, Cuzzocrea S, Crupi R, Interlandi CD. Effect of Cannabidiol (CBD) on Canine Inflammatory Response: An Ex Vivo Study on LPS Stimulated Whole Blood. Veterinary Sciences. 2021; 8(9):185. https://doi.org/10.3390/vetsci8090185

Chicago/Turabian Style

Gugliandolo, Enrico, Patrizia Licata, Alessio Filippo Peritore, Rosalba Siracusa, Ramona D’Amico, Marika Cordaro, Roberta Fusco, Daniela Impellizzeri, Rosanna Di Paola, Salvatore Cuzzocrea, Rosalia Crupi, and Claudia Dina Interlandi. 2021. "Effect of Cannabidiol (CBD) on Canine Inflammatory Response: An Ex Vivo Study on LPS Stimulated Whole Blood" Veterinary Sciences 8, no. 9: 185. https://doi.org/10.3390/vetsci8090185

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