Baculovirus Vectors Induce the Production of Interferons in Swine: Their Potential in the Development of Antiviral Strategies
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Viruses
2.2. Pigs
2.3. Ethics Statement
2.4. Peripheral Blood Mononuclear Cells
2.5. IFN Measurement
2.6. Antiviral Activity Assays
2.7. Recombinant AcMNPV Construction
2.8. Western Blot
2.9. Statistics
3. Results
3.1. AcMNPV Elicits IFN-α-Mediated Antiviral Activity in Porcine PBMCs In Vitro
3.2. AcMNPV Induces Type I and II IFNs and Antiviral Activity in Swine
3.3. Pseudotyping with G-VSV but Not the Enrichment with pCpG Motifs Improves the Ability of AcMNPV to Induce IFN-α In Vitro
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Molina, G.N.; Amalfi, S.; Otero, I.; Taboga, O.; Molinari, M.P. Baculovirus Vectors Induce the Production of Interferons in Swine: Their Potential in the Development of Antiviral Strategies. Vet. Sci. 2021, 8, 278. https://doi.org/10.3390/vetsci8110278
Molina GN, Amalfi S, Otero I, Taboga O, Molinari MP. Baculovirus Vectors Induce the Production of Interferons in Swine: Their Potential in the Development of Antiviral Strategies. Veterinary Sciences. 2021; 8(11):278. https://doi.org/10.3390/vetsci8110278
Chicago/Turabian StyleMolina, Guido Nicolás, Sabrina Amalfi, Ignacio Otero, Oscar Taboga, and María Paula Molinari. 2021. "Baculovirus Vectors Induce the Production of Interferons in Swine: Their Potential in the Development of Antiviral Strategies" Veterinary Sciences 8, no. 11: 278. https://doi.org/10.3390/vetsci8110278