Feline Leishmaniosis in Northwestern Italy: Current Status and Zoonotic Implications
Abstract
:1. Introduction
2. Material and Methods
2.1. Ethical Considerations
2.2. Study Population
2.3. Clinical and Laboratory Diagnosis
2.4. Western Blotting and SDS-Polyacrylamide Gel Electrophoresis
2.5. Preparation of Blood Samples and Extraction of DNA
2.6. Qualitative Polymerase Chain Reaction (PCR) for Detection of Leishmania and Mycoplasma DNA
2.7. Detection of FeLV Antigen and FIV Antibody
2.8. Data Analysis
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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WB | |||
PCR | Negative | Positive | Total |
negative | 118 | 17 | 135 |
positive | 41 | 10 | 51 |
Total | 159 | 27 | 186 |
WB Associate Covariates | ||||||
Qualitative variables | Number of WB positive cats | Number of WB negative cats | p | odds ratio (OR) | Confidence interval (CI 95%) | |
Mycoplasma spp. | Pos | 4 | 5 | 0.02 | 4.39 | 1.078–18.63 |
Neg | 15 | 84 | ||||
PCR associated covariates | ||||||
Qualitative variables | Number of PCR positive cats | Number of PCR negative cats | p | OR | CI 95% | |
FIV | Pos | 23 | 18 | 0.003 | 3. 83 | 1.56–9.41 |
Neg | 12 | 36 | ||||
Quantitative variables | Mean value (min-max) | p | OR | CI 95% | ||
BUN | PCR pos | 119 (18–600) | 0.047 | 1.78 | 1.38–6.27 | |
PCR neg | 93 (5.59–583) | |||||
NEUTR | PCR pos | 10.84 (0.21–37.5) | 0.050 | 2.01 | 1.03–9.58 | |
PCR neg | 8.30 (0.33–22.12) | |||||
HCT | PCR pos | 18.37 (4.6–43.4) | 0.032 | 0.86 | 0.05–0.46 | |
PCR neg | 24.94 (4.2–73.4) | |||||
RBC | PCR pos | 4.04 (0.95–10.12) | 0.048 | 0.78 | 0.01–0.95 | |
PCR neg | 5.34 (1.04–11.65) | |||||
Hb | PCR pos | 6.15 (1.6–14.3) | 0.050 | 0.81 | 0.04–0.93 | |
PCR neg | 8.18 (1.7–27.1) |
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Elmahallawy, E.K.; Zanet, S.; Poggi, M.; Alsharif, K.F.; Agil, A.; Trisciuoglio, A.; Ferroglio, E. Feline Leishmaniosis in Northwestern Italy: Current Status and Zoonotic Implications. Vet. Sci. 2021, 8, 215. https://doi.org/10.3390/vetsci8100215
Elmahallawy EK, Zanet S, Poggi M, Alsharif KF, Agil A, Trisciuoglio A, Ferroglio E. Feline Leishmaniosis in Northwestern Italy: Current Status and Zoonotic Implications. Veterinary Sciences. 2021; 8(10):215. https://doi.org/10.3390/vetsci8100215
Chicago/Turabian StyleElmahallawy, Ehab Kotb, Stefania Zanet, Marco Poggi, Khalaf F. Alsharif, Ahmad Agil, Anna Trisciuoglio, and Ezio Ferroglio. 2021. "Feline Leishmaniosis in Northwestern Italy: Current Status and Zoonotic Implications" Veterinary Sciences 8, no. 10: 215. https://doi.org/10.3390/vetsci8100215