Efficacy of Live Attenuated Vaccine and Commercially Available Lectin against Avian Pathogenic E. coli Infection in Broiler Chickens
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Chicks
2.2. Vaccine and Medication
2.3. E. coli Challenge Isolates
2.4. Experiment Design
2.4.1. Performance Parameters Evaluation
2.4.2. Clinical Signs and Lesion Scoring
2.4.3. Histopathological Examination
2.4.4. E. coli Count on Eosin Methylene Blue (EMB) Agar
2.5. Statistical Analysis
3. Results
3.1. Performance Evaluation
3.2. Clinical and Lesion Scores
3.3. APEC Recovery from Vaccinated and Treated Groups
3.4. Histopathological Lesions
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Groups (25 Birds/Group) | CBL® 1 Vaccine Day Old by Spray | Lector 0.5 mL/L in Drinking Water (18, 19 and 20 Days Old) | Challenge 2 with APEC O78 at Day 21— 0.5 mL of 108 CFU/mL Bird | Challenge with APEC O125 at Day 21 —0.5 mL of 108 CFU/mL Bird | |
---|---|---|---|---|---|
1 | Negative control (NC) | - | - | - | - |
2 | Vaccinated nonchallenged (CBL-V) | + | - | - | - |
3 | Vaccinated challenged with APEC O78 (CBL-V/ O78-C) | + | - | + | - |
4 | Vaccinated challenged with APEC O125 (CBL-V/O O125-C) | + | - | - | + |
5 | Lector before challenge with APEC O78 (Lector+O78-C) | - | + | + | - |
6 | Lector before challenge with APEC O125 (Lector+O125-C) | - | + | - | + |
7 | Positive control APEC O78 challenge (PC-O78) | - | - | + | - |
8 | Positive control APEC O125 (PC-O125) | - | - | - | + |
Groups 1 | Clinical Score 2 | Lesion Score 2 | Mortality | FCR 3 | |||
---|---|---|---|---|---|---|---|
Heart | Liver | Air Sacs | 7 dpi | Cumulative (1–28 Days) | |||
NC | 0 a | 0 a | 0 a | 0 a | 0% | 1.50 | 1.39 |
CBL-V | 0 a | 0 a | 0 a | 0 a | 0% | 1.50 | 1.38 |
CBL-V/O78-C | 1.04 ± 1.48 abd | 0.71 ± 0.69 a | 0.28 ± 0.45 ab | 0.35 ± 0.47 a | 16% | 1.70 | 1.45 |
CBL-V/O125-C | 1.68 ± 1.7 bd | 1.35 ± 0.68 bc | 0.64 ± 0.47 bc | 1.23 ± 0.42 b | 28% | 2.50 | 1.53 |
Lector+O78-C | 0.96 ± 1.56 abd | 0.60 ± 0.71 a | 0.26 ± 0.44 ab | 0.33 ± 0.47 a | 20% | 1.60 | 1.38 |
Lector+O125-C | 0.80 ± 1.46 abd | 0.71 ± 0.69 a | 0.21 ± 0.41 ab | 0.42 ± 0.49 a | 16% | 1.55 | 1.36 |
PC-O78 | 3.40 ± 0.80 c | 1.52 ± 0.49 c | 0.84 ± 0.61 c | 1.52 ± 0.64 b | 60% | 4.00 | 1.59 |
PC-O125 | 2.04 ± 1.70 d | 1.44 ± 0.49 c | 0.83 ± 0.50 c | 1.27 ± 0.44 b | 32% | 3.48 | 1.55 |
DPI 1 | Group 2 | Heart Lesion Scores 3 | Liver Lesion Scores | ||||||
---|---|---|---|---|---|---|---|---|---|
Pericarditis | Myocarditis | Myocardial Necrosis (Hyalinosis) | Necrosis of Hepatocytes | Inflammation in the Portal Area | Inflammation in the Hepatic Parenchyma | Congestion | Glisson’s Capsule Leucocytic Infiltration | ||
2 | NC | - | - | + | - | - | - | + | - |
CBL-V/O78-C | + | + | + | + | +/++ | +/++ | + | - | |
CBL-V/O125-C | ++ | ++ | +++ | + | +/++ | +/++ | ++ | - | |
Lector/O78-C | - | - | + | ++ | ++ | ++ | ++ | - | |
Lector/O125-C | -/+ | + | + | ++ | ++ | ++ | ++ | - | |
PC-O78 | +++ | + | ++ | ++ | ++ | ++ | ++ | - | |
PC-125 | ++ | ++ | ++ | ++ | ++ | ++ | ++ | - | |
7 | NC | - | - | -/+ | -/+ | - | - | -/+ | - |
CBL-V/O78-C | -/+ | + | -/+ | +/++ | +/++ | +/++ | ++ | - | |
CBL-V/O125-C | +/++ | +/++ | +/++ | ++ | ++ | ++ | ++ | - | |
Lector/O78-C | + | -/+ | + | ++ | ++ | ++ | +++ | - | |
Lector/O125-C | -/+ | + | + | ++ | ++ | +++ | ++ | - | |
PC-O78 | +++ | +++ | +++ | +++ | +++ | +++ | +++ | ++ | |
PC-125 | +++ | ++ | +++ | +++ | +++ | +++ | +++ | ++ |
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Abd El-Mawgoud, A.I.; El-Nahass, E.-S.; Shany, S.A.S.; EL-Sawah, A.A.; Dahshan, A.-H.M.; Nasef, S.A.; Ali, A. Efficacy of Live Attenuated Vaccine and Commercially Available Lectin against Avian Pathogenic E. coli Infection in Broiler Chickens. Vet. Sci. 2020, 7, 65. https://doi.org/10.3390/vetsci7020065
Abd El-Mawgoud AI, El-Nahass E-S, Shany SAS, EL-Sawah AA, Dahshan A-HM, Nasef SA, Ali A. Efficacy of Live Attenuated Vaccine and Commercially Available Lectin against Avian Pathogenic E. coli Infection in Broiler Chickens. Veterinary Sciences. 2020; 7(2):65. https://doi.org/10.3390/vetsci7020065
Chicago/Turabian StyleAbd El-Mawgoud, Ahmed I., El-Shayma El-Nahass, Salama A.S. Shany, Azza A. EL-Sawah, Al-Hussien M. Dahshan, Soad A. Nasef, and Ahmed Ali. 2020. "Efficacy of Live Attenuated Vaccine and Commercially Available Lectin against Avian Pathogenic E. coli Infection in Broiler Chickens" Veterinary Sciences 7, no. 2: 65. https://doi.org/10.3390/vetsci7020065