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Acknowledgement to Reviewers of Veterinary Sciences in 2019
Open AccessArticle

In Vitro Antibacterial Activity of Biological-Derived Silver Nanoparticles: Preliminary Data

1
Department of Veterinary Medicine, Università degli Studi di Milano, 26900 Lodi, Italy
2
Department of Biomedical Sciences for Health, Università degli Studi di Milano, 20133 Milano, Italy
*
Author to whom correspondence should be addressed.
Vet. Sci. 2020, 7(1), 12; https://doi.org/10.3390/vetsci7010012
Received: 14 December 2019 / Revised: 10 January 2020 / Accepted: 21 January 2020 / Published: 23 January 2020
(This article belongs to the Special Issue Italian Society of the Veterinary Sciences SISVet 2019)
Silver nanoparticles (AgNPs) are promising alternatives to antibiotics. The aims of this study were to produce AgNPs using two biological methods and determine their antibacterial activity against Pseudomonas aeruginosa and Staphylococcus pseudintermedius. AgNPs were biosynthesized from an infusion of Curcuma longa (turmeric) and the culture supernatant of E. coli. Characterization was achieved by ultraviolet-visible spectroscopy and by Transmission Electron Microscopy (TEM). The antibacterial properties of NPs from C. longa (ClAgNPs) and E. coli (EcAgNPs), alone and in combination with carbenicillin and ampicillin, were investigated through the Kirby-Bauer disk diffusion assay and the minimum inhibitory concentration (MIC). Dimensions of NPs ranged from 11.107 ± 2.705 nm (ClAgNPs) to 27.282 ± 2.68 nm (EcAgNPs). Kirby-Bauer and MIC assays showed great antibacterial abilities for both NPs alone and in combination with antibiotics. EcAgNPs alone showed the most powerful antibacterial activities, resulting in MIC values ranging from 0.438 ± 0.18 µM (P. aeruginosa) to 3.75 ± 3.65 µM (S. pseudintermedius) compared to those of ClAgNPs: 71.8 ± 0 µM (P. aeruginosa) and 143.7 ± 0 µM (S. pseudintermedius). The antibiofilm abilities were strain-dependent, but no statistical differences were found between the two NPs. These results suggest the antibacterial potential of AgNPs for the treatment of infectious diseases.
Keywords: antibiotic resistance; green synthesis; Pseudomonas aeruginosa; silver nanoparticles; Staphylococcus pseudintermedius antibiotic resistance; green synthesis; Pseudomonas aeruginosa; silver nanoparticles; Staphylococcus pseudintermedius
MDPI and ACS Style

Meroni, G.; Soares Filipe, J.F.; Martino, P.A. In Vitro Antibacterial Activity of Biological-Derived Silver Nanoparticles: Preliminary Data. Vet. Sci. 2020, 7, 12.

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