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Mycobacterium avium subsp. paratuberculosis ELISA Responses in Milk Samples from Vaccinated and Nonvaccinated Dairy Goat Herds in The Netherlands

1
Department of Small Ruminant Health, GD Animal Health, P.O. Box 9, 7400 AA Deventer, The Netherlands
2
Epidemiology Group, GD Animal Health, P.O. Box 9, 7400 AA Deventer, The Netherlands
3
Department of Research and Development, GD Animal Health, P.O. Box 9, 7400 AA Deventer, The Netherlands
4
Department of Farm Animal Health, Faculty of Veterinary Medicine Utrecht University, Yalelaan 7, 3584 CL Utrecht, The Netherlands
*
Author to whom correspondence should be addressed.
Vet. Sci. 2019, 6(2), 58; https://doi.org/10.3390/vetsci6020058
Received: 27 April 2019 / Revised: 17 June 2019 / Accepted: 20 June 2019 / Published: 22 June 2019
(This article belongs to the Special Issue Mycobacterial Diseases in Animals)
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Abstract

The aims of our study were to calculate the most appropriate cut-off value for milk samples in a serum-validated Mycobacterium avium subsp. paratuberculosis (MAP) ELISA and to analyze MAP ELISA responses in milk samples from vaccinated and nonvaccinated dairy goats in the Netherlands. Analyzed herds were representative for location and herd size of dairy goat herds in the Netherlands. A significantly higher proportion of the analyzed 49 herds were organic as compared with the total Dutch dairy goat population. First, the MAP ELISA was optimized using 992 paired serum and milk samples. At a cut-off of 25 S/P%, the relative sensitivity (Se) was 58.4% (n = 992, 95% CI: 48.8%−67.6%) and relative specificity (Sp) was 98.5% (n = 992, 95% CI: 97.5%−99.2%), as compared to serum ELISA results. The percentage of positively tested herds was 78.2% (n = 49, 95% CI: 63.4%−88.1%). The percentage of positive milk samples per herd (n = 22) was on average 4.6% (median, min, and max of 4.7%, 0.0%, and 10.7%, respectively). Average age of ELISA-positive (3.2 years) and -negative goats (3.2 years) was not different. Significantly more vaccinated goats tested positive (6.7%) as compared with nonvaccinated goats (1.1%). This study shows that a high number of vaccinated and nonvaccinated commercial dairy goat herds in the Netherlands have MAP-ELISA-positive goats. View Full-Text
Keywords: dairy goat; caprine; antibody response; Netherlands; ELISA; Johne’s disease; paratuberculosis dairy goat; caprine; antibody response; Netherlands; ELISA; Johne’s disease; paratuberculosis
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Luttikholt, S.; Lievaart-Peterson, K.; Gonggrijp, M.; Aalberts, M.; van Schaik, G.; Vellema, P. Mycobacterium avium subsp. paratuberculosis ELISA Responses in Milk Samples from Vaccinated and Nonvaccinated Dairy Goat Herds in The Netherlands. Vet. Sci. 2019, 6, 58.

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