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Vet. Sci. 2019, 6(1), 8; https://doi.org/10.3390/vetsci6010008

Investigation of PAG2 mRNA Expression in Water Buffalo Peripheral Blood Mononuclear Cells and Polymorphonuclear Leukocytes from Maternal Blood at the Peri-Implantation Period

1
Dipartimento di Medicina Veterinaria, Università degli studi di Perugia, 06100 Perugia, Italy
2
Laboratoires d’Endocrinologie Animale et de Reproduction, Faculté de Médicine Vétérinaire, Université de Liège, B-4000 Liège, Belgium
3
CREA Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria, Centro di ricerca Zootecnia e Acquacoltura, 00015 Monterotondo (Roma), Italy
*
Author to whom correspondence should be addressed.
Received: 14 December 2018 / Revised: 8 January 2019 / Accepted: 9 January 2019 / Published: 14 January 2019
(This article belongs to the Special Issue Italian Society of the Veterinary Sciences SISVet 2018)
PDF [414 KB, uploaded 14 January 2019]

Abstract

: The main objective of this study was to assess PAG2 mRNA expression in maternal blood cells at the peri-implantation period in water buffalo; moreover, we wanted to evaluate the earliest time in which PAG-2 could be detected in maternal blood. Thirty-two lactating buffaloes artificially inseminated (AI) were utilized. Blood was collected at Days 0, 14, 18, 28, 40 after AI (AI = day 0). Pregnancy was diagnosed by ultrasound at Days 28 and 40 post AI. Out of 32 buffaloes, 14 were pregnant (P group) and 18 were not pregnant (NP group). The plasma PAG-2 threshold of 1.0 ng/mL in the P group was reached at day 40 post AI. PAG2 mRNA expression differed between the P and NP groups, and was either evaluated in Peripheral Blood Mononuclear Cells (PBMC) or Polymorphonuclear Leukocytes (PMN), starting from day 14. However, both the estimated marginal means and multiple comparisons showed that PAG2 mRNA expression was higher in PMN than PBMC. In the present study, PAG-2 appeared in the blood (40 Days post AI), and an early expression of PAG2 mRNA at Day 14 post AI was also observed. Although further research is undoubtedly required, PAG2 mRNA in peripheral blood leukocytes could be using to better understand the role that PAGs play during pregnancy in buffalo.
Keywords: PAG2 mRNA; peri-implantation; pregnancy diagnosis; water buffalo PAG2 mRNA; peri-implantation; pregnancy diagnosis; water buffalo
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Barbato, O.; Guelfi, G.; Menchetti, L.; Brecchia, G.; de Sousa, N.M.; Canali, C.; Grandoni, F.; Scatà, M.C.; De Matteis, G.; Casano, A.B.; Beckers, J.F.; Barile, V.L. Investigation of PAG2 mRNA Expression in Water Buffalo Peripheral Blood Mononuclear Cells and Polymorphonuclear Leukocytes from Maternal Blood at the Peri-Implantation Period. Vet. Sci. 2019, 6, 8.

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