Comparison of Protein Gut Samples from Rhipicephalus spp. Using a Crude and an Innovative Preparation Method for Proteome Analysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Single Epithelial Cell Dissociation from the Tick Gut Epithelium of R. microplus
2.2. Isolation of Single Epithelial Cells and Purification of Surface Proteins
2.3. LC/MS Analysis of Biotinylated Gut Surface and Crude Gut Proteins
3. Results
Purification of Biotinylated Membrane-Bound Protein and LC-MS/MS Analysis
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Crude Extraction | Membrane-Bound | |
---|---|---|
Total tick proteins | 692 | 1242 |
Total membrane-bound | 108 | 314 |
Total host proteins | 824 | 595 |
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Karbanowicz, T.P.; Nouwens, A.; Tabor, A.E.; Rodriguez-Valle, M. Comparison of Protein Gut Samples from Rhipicephalus spp. Using a Crude and an Innovative Preparation Method for Proteome Analysis. Vet. Sci. 2018, 5, 30. https://doi.org/10.3390/vetsci5010030
Karbanowicz TP, Nouwens A, Tabor AE, Rodriguez-Valle M. Comparison of Protein Gut Samples from Rhipicephalus spp. Using a Crude and an Innovative Preparation Method for Proteome Analysis. Veterinary Sciences. 2018; 5(1):30. https://doi.org/10.3390/vetsci5010030
Chicago/Turabian StyleKarbanowicz, Thomas P., Amanda Nouwens, Ala E. Tabor, and Manuel Rodriguez-Valle. 2018. "Comparison of Protein Gut Samples from Rhipicephalus spp. Using a Crude and an Innovative Preparation Method for Proteome Analysis" Veterinary Sciences 5, no. 1: 30. https://doi.org/10.3390/vetsci5010030