Stability of Porcine Epidemic Diarrhea Virus on Fomite Materials at Different Temperatures
Abstract
:1. Introduction
2. Materials and Methods
2.1. PEDV Propagation and Quantification
2.2. Application of Virus on Fomites and Assessment of Stability
2.3. Quantitative Real-Time RT-PCR
2.4. Immunoplaque Assay
2.5. Statistical Analysis
3. Results
3.1. Survival of Infectious Virus on Fomite Materials
3.2. Immunoplaque Assay
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Fomite Material | Immunoplaque Assay (FFU/mL) | qRT-PCR (Ct Value) † | |
---|---|---|---|
Cultured | Feces | ||
Input virus | 1.0 ± 0.7 × 106 | 17.06 ± 0.77 | 29.65 ± 1.79 |
Room Temperature/2 days * | |||
Cultured | Feces | ||
Styrofoam | <24 | 16.57 ± 0.34 | 30.04 ± 0.38 |
Tyvek coverall | <24 | 16.65 ± 0.12 | 28.61 ± 0.49 |
Cardboard | <24 | 21.50 ± 0.39 | 27.56 ± 0.26 |
4C/20 days * | |||
Cultured | Feces | ||
Styrofoam | 9.2 ± 0.6 × 102 | 17.64 ± 0.51 | 28.89 ± 0.54 |
Tyvek coverall | 5.0 ± 2.5 × 101 | 16.93 ± 0.53 | 27.24 ± 0.75 |
Cardboard | <24 | 17.64 ± 0.22 | 27.69 ± 0.83 |
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Kim, Y.; Krishna, V.D.; Torremorell, M.; Goyal, S.M.; Cheeran, M.C.-J. Stability of Porcine Epidemic Diarrhea Virus on Fomite Materials at Different Temperatures. Vet. Sci. 2018, 5, 21. https://doi.org/10.3390/vetsci5010021
Kim Y, Krishna VD, Torremorell M, Goyal SM, Cheeran MC-J. Stability of Porcine Epidemic Diarrhea Virus on Fomite Materials at Different Temperatures. Veterinary Sciences. 2018; 5(1):21. https://doi.org/10.3390/vetsci5010021
Chicago/Turabian StyleKim, Yonghyan, Venkatramana D. Krishna, Montserrat Torremorell, Sagar M. Goyal, and Maxim C.-J. Cheeran. 2018. "Stability of Porcine Epidemic Diarrhea Virus on Fomite Materials at Different Temperatures" Veterinary Sciences 5, no. 1: 21. https://doi.org/10.3390/vetsci5010021