Pathological Characteristics of Pregnant Tree Shrews Infected by Zoonotic Hepatitis E Virus Genotype and the Effect of Estrogen on Virus Replication
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Animals and Reagents
2.2. Procedure for HEV Infection in Tree Shrews
2.3. Liver Function Analysis
2.4. Estrogen Level Measurement
2.5. Histopathological Examination
2.6. Effect of Estrogen on Kernow-C1 Replication in A549 Cells
2.7. Quantification of HEV Titer
3. Results
3.1. Clinical Observations and Pathological Changes
3.2. Quantification of HEV Replication in Tissues
3.3. Serum ALT and AST Dynamics in Liver Function Tests
3.4. Dynamic of Serum Estrogen
3.5. Histopathological Changes in HEV-Infected Pregnant Tree Shrews
3.6. The Effect of Estrogen on HEV Replication in Cell Culture
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
HEV | hepatitis E virus |
AST | Aspartate aminotransferase |
ALT | Alanine aminotransferase |
A549 | human lung cancer cells |
Huh-7 | human hepatocellular carcinoma cells |
DES | Diethylstilbestrol tablets |
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Zhu, P.; Wang, G.; Punyapornwithaya, V.; Jainonthee, C.; Tian, J.; Liu, Y.; Suksawat, F.; Angkititrakul, S.; Nan, Y.; Li, Z.; et al. Pathological Characteristics of Pregnant Tree Shrews Infected by Zoonotic Hepatitis E Virus Genotype and the Effect of Estrogen on Virus Replication. Vet. Sci. 2025, 12, 483. https://doi.org/10.3390/vetsci12050483
Zhu P, Wang G, Punyapornwithaya V, Jainonthee C, Tian J, Liu Y, Suksawat F, Angkititrakul S, Nan Y, Li Z, et al. Pathological Characteristics of Pregnant Tree Shrews Infected by Zoonotic Hepatitis E Virus Genotype and the Effect of Estrogen on Virus Replication. Veterinary Sciences. 2025; 12(5):483. https://doi.org/10.3390/vetsci12050483
Chicago/Turabian StyleZhu, Peiying, Guojun Wang, Veerasak Punyapornwithaya, Chalita Jainonthee, Jijing Tian, Yan Liu, Fanan Suksawat, Sunpetch Angkititrakul, Yuchen Nan, Zailei Li, and et al. 2025. "Pathological Characteristics of Pregnant Tree Shrews Infected by Zoonotic Hepatitis E Virus Genotype and the Effect of Estrogen on Virus Replication" Veterinary Sciences 12, no. 5: 483. https://doi.org/10.3390/vetsci12050483
APA StyleZhu, P., Wang, G., Punyapornwithaya, V., Jainonthee, C., Tian, J., Liu, Y., Suksawat, F., Angkititrakul, S., Nan, Y., Li, Z., Duan, X., & Li, W. (2025). Pathological Characteristics of Pregnant Tree Shrews Infected by Zoonotic Hepatitis E Virus Genotype and the Effect of Estrogen on Virus Replication. Veterinary Sciences, 12(5), 483. https://doi.org/10.3390/vetsci12050483