Spontaneous Lesions of Endangered Geriatric Julia Creek Dunnarts (Sminthopsis douglasi, Archer 1979) with Emphasis in Reproductive Pathology

Simple Summary

This study focuses on the spontaneous lesions observed in a captive colony of geriatric Julia Creek dunnarts, an endangered carnivorous marsupial. Despite their conservation status, no prior research has focused on the conditions affecting captive individuals attaining senescence. We examined one wild and thirty-five captive-born, mostly elderly dunnarts that failed to reproduce over several breeding periods. From these, ten dunnarts had normal findings. Among females, the most common issue was cystic glandular hyperplasia (eight cases); cutaneous lesions were infrequent (two cases). Males showed testicular degeneration, aspermatogenesis, or atrophy (three cases). Cutaneous lesions compatible with epitheliotropic T cell lymphomas were observed in both sexes (five cases) and thus, an underlying oncogenic viral etiology is suspected. This is the first study that documents spontaneous diseases in aging Julia Creek dunnarts, shedding light on geriatric conditions within a conservation context.

Abstract

Julia Creek dunnarts are an endangered species of carnivorous marsupials and the focus of multiple conservation strategies involving significant resources such as captive breeding programs. Despite the relevance for conservation, no study to date has focused on evaluating geriatric diseases in dunnarts. This study describes the pathology findings in a group of one wild and thirty-five captive-born, mostly geriatric Julia Creek dunnarts that failed to produce offspring over multiple breeding periods. A total of 20 females and 16 males were submitted for a postmortem examination, with ages ranging from 9 to 42 and 12 to 42 months for females and males, respectively. Of these, 10 had unremarkable findings. The most common condition in females was cystic glandular hyperplasia (n = 8), typical of hormonal dysregulation profiles in senescence, particularly hyperestrogenism. Rarely, cutaneous disease represented by unidentified dermal round cell infiltrates was observed in females (n = 2). Primary reproductive hormonal dysregulation was also suspected in males diagnosed with testicular degeneration, aspermatogenesis and/or atrophy (n = 3). Cutaneous round cell infiltrates, possibly compatible with epitheliotropic lymphomas, were seen in males (n = 3), and 2/3 affected males also had concurrent testicular degeneration or atrophy, indicating male sex could be a predictor for lymphoid neoplasia in aged dunnarts, especially in individuals with concurrent testosterone-luteinizing hormone dysregulation as it occurs in gonadectomized animals. The role of an underlying viral etiology is also explored. This study is the first to describe major spontaneous diseases in endangered aged Julia Creek dunnarts, providing an important understanding of senescence and geriatric diseases within a conservation context.

1. Introduction

Captive breeding programs have been used to counteract wildlife population declines, maintain gene pool diversity, achieve threat mitigation and reintroduce future self-sustaining populations. Such programs have been key in the recovery of multiple species such as the Arabian oryx (Oryx leucoryx), California condor (Gymnogyps californianus) and the golden lion tamarin (Leontopithecus rosalia), amongst others [1,2,3]. However, captive breeding is considered a last resort conservation tool due to the inexorable behavioral, phenotypical and genetic challenges faced by wildlife in captive environments. Successful establishment of captive breeding populations is challenging and requires careful planning to evoke an appropriate environment to achieve successful reproduction, major logistics, long-term financial and administrative continuity as well as avoiding domestication. These conditions are necessary to secure the maintenance of healthy individuals and ensure successful reintroductions and long-term maintenance of genetically viable populations.

Captive breeding has been one of the conservation tools used to secure healthy breeding stocks of Julia Creek dunnarts (Sminthopsis douglasi), a small nocturnal Australian carnivorous marsupial of the family Dasyuridae endemic to the state of Queensland. Longevity is similar to other small marsupials; wild male and female Julia Creek dunnarts live up to 12 and 24 months, respectively [4], with records extending to three years in captivity. Typically, they are found in low densities [5] and their habitat includes grassland ecosystems with heavy clay soils and a rich diversity of invertebrate and vertebrate prey [4]. The species was considered extinct in the 1980s and was re-discovered from owl pellets, cat remains and by the capture of live specimens a decade later [6,7]. Currently, Julia Creek dunnarts are listed as vulnerable under the Environment Protection and Biodiversity Conservation Act 1999 and Endangered under the Nature Conservation Act 1992 in Queensland [5], with feral cats (Felis catus) and invasive weeds (e.g., prickly acacia, Vachellia nilotica) outcompeting understory vegetation and heavy livestock grazing as important threats [8]. Additional threats include extreme weather from climate change, as Julia Creek dunnarts abundance patterns are positively correlated to rainfall, which directly influences understory cover growth, which in turn provides shelter from potential predators [4]. Significant resources have been invested to bring forward a conservation plan for the species [9] which does not include disease management despite the documented predisposition of dasyurids to proliferative diseases [10]. Thus, diseases are not currently considered a threat to the species and no active measures to investigate or prevent the occurrence of common diseases have been proposed.

Similar to other wildlife managed in captive environments, Julia Creek dunnarts are vulnerable to potential disease outbreaks and age-related diseases as they easily outlive wild conspecifics. Due to the direct impact of spontaneous geriatric diseases on their conservation, this manuscript focuses on documenting the reproductive and non-reproductive lesions observed in geriatric Julia Creek dunnarts held in a captive breeding colony.

2. Materials and Methods

2.1. Population and Data Collection

The colony was initially formed in the 1990s following the capture of eight wild-caught founder individuals that were held at La Trobe University prior to their transfer to the Pearcedale Conservation Park (PCP) in Victoria [6,9], where an additional eight wild-caught dunnarts from Bladensburg National Park were transferred to the captive colony in April 2001 [6]. In August 2002, 12 dunnarts (3 M:9 F) were transferred from PCP to David Fleay Wildlife Park (DFWP) on the Gold Coast in Queensland, Australia. In August 2006, a male was introduced into the captive colony and was the last wild-caught dunnart to be introduced into the colony. The captive colony was transferred from DFWP to The University of Queensland (UQ) Native Wildlife Teaching and Research (NWT&R) Facility on the Gatton Campus in March 2009 (Animal Ethics permits SAS/930/08/BREED, SAFS/441/11/BREED; Queensland Government permit WIEP05809909).

Reproductive management of the captive colony at UQ involved monitoring female dunnarts for signs of estrus (increased presence of epithelial cells in urine, running wheel activity and feed residue) and were paired for breeding. Following the placement of the male in the female’s enclosure (n = 298), mating occurred in approximately one-third of the introductions from 2009 to 2014. A total of 11 litters (47 joeys) were produced at the NWT&R Facility with 98% surviving to weaning.

The colony in the UQ NWT&R Facility rapidly attained senescence with multiple individuals reaching up to 3.5 years (lifespan 1–2 years) failing to produce offspring, despite observed matings across multiple breeding seasons. The absence of new genetics into the colony contributed to inbreeding depression, resulting in the disbanding of the colony in early 2014. Dunnarts eligible for inclusion in this study were found dead aged >1 year or euthanized due to age and/or signs of disease (e.g., progressive loss of body condition, abdominal distension, lethargy, alopecia), which failed to reproduce upon reaching senescence and received postmortem examination. There were two subsets of dunnarts. The first subset included those that had received a postmortem examination and follow-up histopathology at the time of death/euthanasia (1991–2014, n = 17). Detailed postmortem examinations were performed in 10 cases and histopathology was conducted in sections of formalin-fixed tissues received in an additional seven cases from offsite autopsies. Relevant case material was procured from archived samples from the UQ Pathology Department. The second subset comprised those that had died/been euthanized between 2009 and 2012, preserved at −20 °C (n = 19), and autopsied after a slow thawing process at 4 °C in April 2023. Details about origin, postmortem workup, broad lesion category and demographics are included in Supplementary Table S1.

2.2. Gross Examination, Sample Collection and Processing

A detailed postmortem examination was conducted in each dunnart on the first subset following UQ’s standard operating procedures. Representative samples of main organs such as brain, lungs, heart, liver, spleen and/or lymph nodes, stomach, intestine, kidneys, urinary bladder, skeletal muscle, reproductive tract (ovaries, uteri, cervix and vagina, testes and prostate) were collected and fixed in 10% buffered formalin prior to routine histopathology processing, to be paraffin-embedded, sectioned at 4 um, mounted on glass slides, and stained with hematoxylin and eosin (H&E) stain. Due to the expected severe freezing artefact and lack of funding to cover for histopathology of all main organs, histopathology was only carried out in the reproductive tract and skin in the second subset of dunnarts showing gross lesions.

2.3. Ancillary Testing

Immunohistochemistry (IHC) methods for markers targeting pan-leukocytic cellular populations (CD68), T-lymphocytes (CD3), B-lymphocytes (CD20, CD79a, Pax-5), histiocytic cells (Iba-1), hematopoietic tissue (CD45 LCA) or epithelial cells (AE1/AE3) were carried out according to published protocols [11]. Details about IHC protocols can be found in Supplementary Material S1. Photomicrographs obtained during the validation procedures for each marker can be found in Supplementary Figures S1–S8. Histochemistry (Gram, Toluidine blue) was performed in a case-by-case basis following UQ’s and external laboratory standard laboratory protocols.

3. Results

Main demographic data, reproductive tract and significant non-reproductive tract morphological diagnoses are included in

Table 1. Female reproductive and non-reproductive tract lesions in endangered geriatric Julia Creek dunnarts from a captive breeding colony.

Diagnoses	Dunnart ID	Age (Months)
Ovaries
Cystic ovary (possibly bursal)	6	25
Age-related ovarian atrophy	8	25
Age-related ovarian atrophy	10	42
Oviduct
Cystic oviduct	10	42
Uterus
Cystic glandular hyperplasia, squamous metaplasia	3	27
Cystic glandular hyperplasia/dysplasia, squamous metaplasia	7	25
Cystic glandular hyperplasia with mucin	8	25
Cystic glandular hyperplasia	2	24
Cystic glandular hyperplasia	10	42
Cystic glandular hyperplasia	24	24
Cystic glandular hyperplasia, polyp (glandular)	27	24
Cystic glandular hyperplasia, squamous metaplasia	11	20
Cystic glandular hyperplasia, squamous metaplasia, polyp (glandular)	14	42
Endometrial polyp (glandular)	23	12
Endometrial polyp (glandular), cystic	30	24
Endometrial polyp (glandular), cystic	32	24
Endometrial adenoma, papillary, focal squamous metaplasia	13	42
Cervix and vagina
Neutrophilic infiltrates in cervix and vagina with fibrosis	8	25
Neutrophilic infiltrates in vagina	10	42
Skin
Round cell infiltrates, superficial dermis, non-epitheliotropic	30	24
Round cell infiltrates, mid-to-deep dermal, epitheliotropic	33	12

and

Table 2. Male reproductive and non-reproductive tract lesions in endangered geriatric Julia Creek dunnarts from a captive breeding colony.

Diagnoses	Dunnart ID	Age (Months)
Testes
Testicular tubular degeneration/atrophy with aspermatogenesis	4	42
Testicular tubular degeneration/atrophy with aspermatogenesis and metastatic lymphoma	5	42
Testicular tubular degeneration/atrophy	9	42
Prostate
Prostatic mineralization, multifocal	9	42
Lymphoplasmacytic prostatitis	12	42
Skin
Cutaneous epitheliotropic lymphoma (mycosis fungoides)	1	24
Cutaneous epitheliotropic lymphoma (mycosis fungoides), with splenic and pulmonary metastasis	4	42
Cutaneous epitheliotropic lymphoma (mycosis fungoides), with splenic, pulmonary and testicular metastasis	5	42
Cutaneous round cell infiltrates, telogen alopecia	25	48
Cutaneous round cell infiltrates	31	48
Telogen alopecia, hyperkeratosis	20	36
Telogen alopecia	28	24
Telogen alopecia	29	24

and in Supplementary Table S1. A total of 36 dunnarts from the colony were submitted for a postmortem examination: 20 females and 16 males from 2009 through 2022. From these, five geriatric males, one adult non-geriatric male and four adult, non-geriatric females were excluded from analyses as their gross and histopathological examinations were unremarkable or had non-reproductive lesions (e.g., cryptococcosis in male dunnart 127) or their histopathology examination was outsourced (fibrosarcoma in the hindlimb of male dunnart 176). Few dunnarts had good to excellent (n = 5) or adequate/moderate body condition (n = 6). Poor body condition was recorded in nine dunnarts. Body condition was not disclosed in the clinical history of five dunnarts. Weight was recorded in the second subset of autopsies with dunnarts being in normal to excellent body condition when weighing around >52 g (n = 9), whilst those in poor body condition weighing often <50 g (n = 10).

For the second subset of dunnarts in the present study (n = 14), there was a similar mating success (33.33% from 168 introductions) in the colony. Only two litters (six joeys) were produced, resulting in a lower birthing success; however, all six offspring survived to weaning.

3.1. Gross Pathology—Female Reproductive Tract

Lesions were often found affecting the uteri, mainly represented by cystic glandular hyperplasia (CGH, Figure 1a–c), and less frequently by cysts affecting other parts such as the oviduct or ovaries (Figure 1d). Reproductive inflammation was uncommon and was mostly represented by neutrophils (Figure 1e). No cases of malignant neoplasms were observed. Grossly, when the reproductive tract was affected by cystic disease, lesions were more often evident in the endometrium (dunnarts 3, 7–8, 10–11, 14, 24, 27), rarely accompanied by cystic expansion of adjacent anatomy such as the oviduct (cystic oviduct; dunnart 10) or ovary only (ovarian cyst Not Otherwise Specified—NOS; dunnart 6). Ovarian cyst NOS were described as having unilateral severe distension, and the oviducts as having one enlarged 5–7 mm diameter, light yellow-pink, soft, fluid-filled cyst each mostly in the proximal end. No macroscopic lesions were observed in the external genitalia in any of the females in this study. In general, and for those individuals for which information was recorded, disease of the reproductive tract in females was more often unilateral (n = 14) in comparison to bilateral (n = 3).

3.2. Histopathology—Female Reproductive Tract

CGH was the most commonly diagnosed finding (Figure 2c–f) and was characterized by numerous proliferating, often cystic, endometrial glands. The stroma was reduced in comparison with the lesser affected portions of the endometrium. Additionally, in two dunnarts with CGH, anovulatory ovaries were found, deemed to be age-related atrophy cases (Figure 2a). CGH with concurrent squamous metaplasia was observed in four cases (Figure 2e), usually observed as focal lesions only co-occurring within CGH tissue. Endometrial (glandular) polyps were found in three cases (Figure 2f), containing numerous endometrial-like glandular profiles. Although oviductal lesions were uncommon in this study, a cystic oviduct was observed (Figure 2b) causing luminal expansion and degeneration of the oviductal mucosa. A single case of an endometrial adenoma was observed (Figure 2g,h) and was diagnosed as a papillary type, with numerous mucin-producing cells resulting in the production of large amounts of mucin. Findings in the cervix and vagina were infrequent (two cases) and were represented by neutrophilic infiltrates, one case along fibrosis (Figure 2i).

3.3. Additional Significant Non-Reproductive Disease—Female Dunnarts

From the second subset, two dunnarts presented cutaneous round cell infiltrates: dunnarts 30 and 33. In detail, dunnart 30 had a cystic glandular uterine polyp and also presented with bilaterally, markedly thickened skin with alopecia and with focal, asymmetrical/unilateral erosions grossly, which histologically corresponded to a moderate proliferation of round cells lacking epitheliotropism but infiltrating and separating the dermal collagen in an equivocal sheet pattern. Dunnart 33 did not have concurrent reproductive lesions and was diagnosed instead with deep to mid-dermal round cell infiltrates that were not immunolabeled by any of the immunohistochemical markers used, as well as with cutaneous erosions and severe alopecia.

3.4. Gross Pathology—Male Reproductive Tract

Grossly visible lesions were only seen in the testes and scrotum. No gross lesions were observed for the epididymis, ductus deferens, bulbourethral glands or penis. Main lesions observed were primarily degenerative, represented by testicular degeneration/atrophy (Figure 3a), and causing a dramatic reduction in the length of the inguinal canal compared to a control (Figure 3b). Asymmetrical testes were noted in two cases, one of which was confirmed as bilateral testicular tubular degeneration/atrophy, likely in combination with autolysis (Figure 3c). For those individuals in which information was recorded, reproductive disease was more often unilateral (n = 3) in comparison to bilateral (n = 1) as seen in females.

3.5. Histopathology—Male Reproductive Tract

Testicular tubular degeneration/atrophy with aspermatogenesis (Figure 4a–c) were diagnosed in two males, one of which also had a primary cutaneous epitheliotropic lymphoma resulting in a splenic, pulmonary and unilateral testicular metastasis (Figure 4d). In these cases, common features included tubules being either segmentally/partially or diffusely depleted from germ cells, and lined by the highly resistant Sertoli cells, where postmortem preservation was adequate. Exfoliated individualized or multinucleated germ cells were observed within tubules containing some viable germ epithelium. In cases of either bilateral testicular atrophy (Figure 4e) or absence of palpable intra-scrotal testes (Figure 4f), degenerative lesions were also observed in the epididymis. Lesions in the prostate were mild and represented by interstitial inflammatory infiltrates (Figure 4g) or intratubular calcified concretions (Figure 4h).

3.6. Additional Significant Non-Reproductive Disease—Male Dunnarts

In the first subset, significant non-reproductive disease in males was represented by three cases (dunnarts 1, 4, and 5) of primary cutaneous epitheliotropic lymphoma, resulting in severe and extensive gross cutaneous lesions (Figure 3d,e) compared to a lymphoplasmacytic dermatitis of unknown origin (Figure 3f). Telogen alopecia was also observed in four geriatric males (Figure 3g,h) occasionally along comedone formation (Figure 3i). In the second autopsied subset of dunnarts, two additional male dunnarts (dunnarts 31 and 33) were found with round cell infiltrates in the dermis, all manifesting in a clear sheet pattern but with dunnart 31 lacking epitheliotropism. On histopathology, the cutaneous epitheliotropic lymphoma cases presented with a densely cellular neoplasm arranged in sheets (Figure 5a) expanding the dermis, reaching the subcutis, and multifocally infiltrating the follicular epithelium (Figure 5b) extending to the epidermis filling vesicles (Figure 5c).

3.7. Histochemistry and Immunohistochemistry (IHC)

Toluidine blue (TB)—a metachromatic dye that stains cytoplasmic heparin granules in mast cells a deep purple color—was run in the second group of autopsied dunnarts that had cutaneous round cell infiltrates as there were frequently larger, rounded cells accompanying the primary infiltrating population. These larger cells corresponded to mast cells and their metachromatic granules were highlighted with TB. Regarding IHC controls, dunnart nodal tissue had adequate immunolabeling patterns of paracortical T (CD3) and cortical B cell (CD20, CD79a, Pax-5) markers, and IHC recovery was similar in all samples. However, there was minimal pan-leukocytic (CD68), histiocytic (Iba-1) or hemopoietic tumor markers’ (CD45 LCA) reactivity in control tissue. Pan-cytokeratin labeling was adequate in control tissue, and negative in the cells inducing the cutaneous round cell infiltrates.

3.8. Non-Reproductive, Non-Clinically Significant Findings—Geriatric Male and Female Dunnarts

In the first subset, non-reproductive lesions were also observed but was generally considered of little or with no clinical significance (e.g., mild inflammatory infiltrates in stomach, intestine, kidneys, and/or liver—five cases combined; mild hepatocellular lipidosis—two cases; or mild hepatic glycogen storage—one case), associated with senile changes (e.g., multifocal mild renal mineralization or fibrosis—three cases; hepatic lipofuscinosis—one case) or euthanasia artefacts (e.g., pulmonary hemorrhages, congestion and edema—seven cases).

4. Discussion

In captivity, female Julia Creek dunnarts become sexually mature between 17 and 27 weeks and males between 28 and 31 weeks [4]. Similar to other dasyurids, dunnarts have evolved reproductive strategies to maximize fitness within their short lifespans (2–3 years), such as being seasonally polyestrous, an avoidance of semelparity (rapid senescence and death after first breeding season as in Antechinus sp.), having a spontaneous ovulation, multiparity and the ability to store sperm, which are unique amongst marsupials [12,13]. In addition, in situ surveys have also found that Julia Creek dunnarts can raise two litters in an extended breeding season, which is a common reproductive trait in species inhabiting semi-arid ecosystems where food availability is unpredictable [4]. Despite these adaptations, captive dunnarts unable to breed rapidly attain senescence, increasing their vulnerability to aging-associated conditions as observed in this study. Photoperiod cues could offer some insight into reproductive failure, particularly in male dasyurids. As photoperiod increases in the southern hemisphere, plasma cortisol levels do so as well, negatively influencing spermatogenesis [14]. Mortality coincides in certain dasyurids with elevated post-mating plasma cortisol levels as well as a testosterone-dependent, post-mating decrease in plasma corticosteroid-binding globulin. Such reproductive strategy might bring positive, short-term reproductive benefits for species evolved to have few mating encounters, via increasing energy mobilization. However, it can also induce fatal syndromes resulting in anemia, anorexia, hippocampal neuronal apoptosis, increased vulnerability to parasitic and bacterial infections, and decreased splenic follicle size, amongst other issues. The role of reproduction in post-mating morbidity and mortality is further supported by the significant increase in lifespan upon castration or pre-mating removal of males [14].

Susceptibility to age- and non-age-related hyperplasias, benign and malignant tumors vary considerably between marsupials or dasyurids. Interestingly and according to the literature, dasyurids are overrepresented in terms of neoplastic disease compared to other mammals, with one study reporting an incidence of 46% in this family [10] compared to 2–10% reported for other mammals [15]. None of the reported tumors of high incidence in dasyurids or other marsupials such as adenocarcinomas, mammary carcinomas, trichoepitheliomas, squamous cell carcinomas, hemangiomas or hemangiosarcomas, and hepatic adenomas were observed in this study, with the exception of lymphoma [14,16] and one case of a fibrosarcoma in a hind limb (not included in this study). Neoplasia in Julia Creek dunnarts was only represented by multiple cutaneous lymphomas and a uterine adenoma. Additionally, the literature reports degenerative conditions in geriatric dasyurids primarily affecting nervous or musculoskeletal system. Aged quolls (Dasyurus sp.) and Tasmanian devils (Sarcophilus harrisii) (>3 years old) acquire progressive leukoencephalopathies, myelopathies and intervertebral disc disease [17]; these were not observed in the dunnarts in this study.

It is important to note that the present study had a relatively small sample size and unfortunately not all dunnarts received a thorough postmortem examination with histopathology. These factors could have negatively influenced our ability to have a more accurate demonstration of geriatric dunnart diseases. Nonetheless, the literature on dunnart diseases is very limited compared to other marsupials. This fact is not surprising as diseases are not considered a significant threat to dunnart conservation, compared to other dasyurids with well-studied diseases such as the case with Tasmanian devils [18], or other marsupials, such as koalas (Phascolarctos cinereus) [19]. This fact has understandably re-directed research efforts into mapping conservation-priority habitat or answering ecology or biology questions [4,7,12,20]. A few studies regarding naturally occurring disease in captive fat-tailed dunnarts (S. crassicaudata) documented dermal spindle cell tumors, splenic lymphomas, squamous cell carcinomas and round cell sarcomas of the upper limb [10], whilst others stressed the vulnerability of captive dunnarts to the reverse transmission of zoonotic pathogens when human-derived Helicobacter pylori infection resulted in an outbreak of gastric bleeding and weight loss [21]. The cause of the high incidence of neoplasia is currently unknown, but genetic predisposition to lymphoma has been suggested in fat-tailed dunnarts, which grants further exploration of this question in dunnart species [10].

A small amount of information is available in the peer-reviewed literature on ovarian tumors in marsupials, or more specifically, dasyurids, with scattered reports of ovarian hemangiomas and adenocarcinomas in quolls and koalas [22,23]. Most of the lesions found in the female reproductive tract of these dunnarts are considered degenerative changes, namely CEH and cystic oviduct, which, as in other species, are associated with long-term exposure to female reproductive hormones in non-castrated animals [24]. Interestingly, this prolonged exposure is also a risk factor for mammary neoplasia, which was not observed in this study despite being reported in other marsupials [16,25]. The reasons behind this discrepancy are unknown. It is worth noting that in our study, 5 of 11 female dunnarts that did not meet the inclusion criteria (autopsy at NWT&R with no histopathology) also had gross ovarian lesions (dunnarts 134, 159, 170, 200), illustrating the high frequency of reproductive lesions in this colony.

As reported elsewhere in possums [16,23], CGH was the most common disorder of growth in aged female dunnarts, as in aged rabbits, mice, rats and dogs [26,27,28]. Uterine cysts and uterine metaplasia have also been reported in mature gray short-tailed possums (Monodelphis domestica) [29]. Malignant neoplasia appears to be of common occurrence in the marsupial literature [10,30], and from these, carcinomas occur but are more often associated with other tissues (e.g., pulmonary, mammary gland) rather than endometrium [16,30,31,32,33]. Interestingly, primary malignant reproductive neoplasia was not observed in this study, and likewise, it was found to not be the major cause of death in a captive colony of endangered mountain pygmy-possum (Burramys parvus), with the report focusing instead on progressive renal disease [25]. Endometrial carcinomas are uncommon in animals except for the rabbit, cattle and certain rat strains. Multiple parallels regarding reproduction have been identified between rabbits and dunnarts which might provide insights into the pathogenesis of reproductive disorders in dunnarts. The two most common uterine disorders in rabbits are adenocarcinomas—affecting rabbits at any age and commonly resulting in pulmonary metastases—and CGH, with endometrial inflammation being uncommon [34,35]. CGH was the only entity frequently observed in this study. Notably, vaginal serosanguineous discharge and abdominal distension were frequent clinical findings in captive Julia Creek dunnarts with CGH and are also observed in rabbits with adenocarcinoma [35,36]; thus, it is recommended that any future captive breeding attempt in the species incorporates frequent checkups for either of these two clinical signs, with culling being a suitable endpoint. Ovariohysterectomy is the only successful treatment to date, with an overall post-surgical survival of 80% in rabbits [35]; however, this would not be a viable treatment option for a captive breeding colony, where fertility is important. In rabbits with endometrial adenocarcinoma, the non-carcinomatous foci in the endometrium have evidence of CGH, suggesting an association with hyperestrogenism. As induced ovulators, both rabbits and dunnarts can have quite prolonged estrus and experience little luteal activity, resulting in a persisting estrogen/progesterone ratio favoring estrogens [37]. At least in the rabbit, there is evidence of CGH evolving to malignant anaplastic carcinoma [36], so it is unknown if given sufficient time, the CGH dunnart cases would have turned malignant. Future research in this area evaluating the degree of hormone dependence of endometrial carcinomas and CGH could help in understanding the pathogenesis of spontaneous proliferative reproductive disorders in dunnarts.

Both cases of neutrophilic infiltration in cervix and vagina could suggest an acute ascending bacterial infection—despite bacteria not observed on histopathology—or alternatively, their presence is possibly contingent to the phase of the ovarian cycle. Vaginal and cervical inflammation have been reported before in marsupials, mostly due to ascending bacterial infections from the cloaca (site of convergence for urogenital and alimentary tract) or due to tears during mating with an overly aggressive male [38]. Equally, the presence of granulocytes/neutrophils—along cornified and nucleated epithelial cells—in vaginal and cervical segments is a normal finding in mice and rats during metestrus and diestrus, serving as an arm of localized innate immunity and assisting when staging vaginal smears [39,40]. It is unknown to the authors if the dasyurid ovarian cycle behaves similarly to rodents. The literature on the cellular reproductive profile in other marsupials (e.g., Tasmanian rat-kangaroo (Potorus tridmtylus) [41] and Common brushtail possum (Trichosurus vulpecula)) [42] suggests that the ovarian cyclic phase might influence rodents and marsupials in a similar fashion. Nonetheless, it is worth noting that both female dunnarts in which neutrophilic infiltration was observed had concurrent CGH and evidence of anovulatory ovaries, which may favor a pathologic cause for neutrophilic recruitment.

Cases of testicular degeneration are consistent with advanced age as reported elsewhere in other aged male marsupials [29]. In one case (dunnart 9), the left testicle was unilaterally enlarged, necrotic and with a diffusely edematous tunica albuginea, suggesting that the necrosis was secondary to impaired thermoregulation [43]. Testicular atrophy is seen as an advanced state of degeneration, with mineralization and fibrosis. Causes are multiple; however, intrinsically, aging is a well-recognized predisposing factor in all species, probably due to degenerative vascular lesions in testis or pampiniform plexus [43]. Aggregates of interstitial (Leydig) cells were observed in one case of testicular degeneration with concurrent seminiferous tubular loss and replacement by granulomas within a background of autolysis. It is possible that the loss of tubular profiles mimicked interstitial cell hyperplasia, a recognized phenomenon in aged animals with or without atrophied testes. If the cellular proliferation was a real change, hyperplasia would have been the more likely interpretation instead of adenoma, as the diameter of the proliferating foci was less than three seminiferous tubules [44]. It is also worth noting that testicular interstitial cells are prominent in marsupials, particularly in American marsupial families (and Australian bandicoots), where the proportion of testicular volume occupied by the interstitial cells can reach up to 20%, known as a type 3 organization [45]. Although most Australian marsupials are documented to have a type 2 organization, occupying about 5% of testicular volume [45], some dasyurids such as Antechinus stuartii are known to have two separate interstitial cell populations, with one forming peritubular clusters thought to correspond to renewed cells in adulthood [46]. It is unknown if this characteristic is shared by all dasyurid males [47], but once again, such peritubular clustering could have mimicked hyperplasia. Although the functional significance and phylogenetic connotations of such changes are not unknown, familiarity of the anatomic pathologist with such anatomical variations is of utmost importance to perform adequate histological examinations in non-conventional species.

Prostatitis has been reported in sugar gliders (Petaurus breviceps) and Virginia opossums (Didelphis virginiana), mostly associated with urinary tract infection, resulting in hematuria, constipation and local pain; none of which were observed in this study perhaps due to the mild nature of the inflammation observed in dunnart 11 [31,38]. Sexually-transmitted, cytomegalovirus-associated prostatitis has been reported in other Australian dasyurids, the brush-tailed phascogale (Phascogale tapoatafa), the brown antechinus (Antechinus stuartii) and the dusky antechinus (A. swainsonii; [48,49]). As with other cytomegaloviruses, lesions were most frequent during stressful periods. If the inflammation observed in dunnart 11 was virally induced, it is unlikely that viable virions would still be observable in the tissue due to chronicity. Nonetheless, future studies should consider a potential cytomegalovirus infection in dasyurid cases showing prostatic compromise. Prostatic mineralization is considered a senile change lacking clinical significance.

Cutaneous lymphoma was frequent in male dunnarts. There were two females in which both gross and histopathology findings could support a putative diagnosis of lymphoma; however, this diagnosis could not be confirmed due to the severe artefact present in the tissue, impeding confirmatory testing. Thus, we will only discuss cutaneous epitheliotropic lymphoma in the male cohort. Dunnarts with cutaneous epitheliotropic lymphomas presented with extensive alopecia with crusting and ulcerative lesions in the skin. Interestingly, lesions were remarkably similar in distribution and severity, which could indicate a viral etiology. Oncogenic viruses, specifically those of the retroviral lineage, such as the Koala Retrovirus and Gunnison’s Prairie Dog Retrovirus are associated with lymphoid neoplasia in wildlife in Australia and elsewhere [50,51]. This hypothesis might be worth exploring in future studies. Cutaneous epitheliotropic T-cell lymphoma (ETL) is primarily a disease of aged animals and has been reported in a variety of marsupials [52,53] and dasyurids, including Tasmanian devils [54]. In dogs, lesions develop from a patch displaying significant epitheliotropism into a tumor stage with metastasis in as little time as 5.5 months [55], with cutaneous forms (as the dunnarts in this study) considered independent predictors of poor survival compared to mucocutaneous/mucosal forms [56]. The ETL disease spectrum is divided into mycosis fungoides (MF; most common form, strong tropism for epidermis and adnexa), pagetoid reticulosis (MF with lymphadenopathy and circulating neoplastic lymphocytes) and Sézary syndrome (solitary plaque; [57]). According to this classification, the three ETL cases in this study correspond to MF due to the presence of (1) intraepidermal vesicles containing pleomorphic neoplastic lymphocytes (Pautrier’s microabscesses), (2) infiltration of follicles and adnexa (epitheliotropism), and (3) pleomorphic infiltrate predominantly comprising lymphocytes with occasional histiocytes and granulocytes. Predisposing factors to cutaneous ETL are not known; however, chronic inflammation and immune dysregulation, as it occurs in atopic dermatitis cases, is noted as a risk factor for dogs [58]. It remains to be determined if chronic dermatitis, as diagnosed in one male excluded from the study, predisposes aged dunnarts to cutaneous ETL. Although speculative, another possible risk factor for dunnart lymphoma occurrence is the male sex, as indicated by previous studies in neutered dogs, and humans [59,60], suggesting an endocrine dysregulation component. Interestingly and perhaps unsurprisingly, two of the three dunnarts with ETL also had concurrent testicular degeneration, which would lead to low circulating testosterone levels and, subsequently, supraphysiologic luteinizing hormone (LH) levels due to the absence of negative feedback to the hypothalamus and anterior pituitary axis, as would happen in gonadectomized animals. LH has receptors in reproductive and non-reproductive tissues such as lymphocytes and lymphoid tissue (thymic medulla); the function of these receptors is still not fully understood but appears to relate to the induction of cell function and division [61], with the latter via an ERK-dependent pathway [62]. High LH would lead to a constant activation and magnification of LH effects in non-reproductive tissues, including T lymphocytes [63]. LH-receptor positive T lymphocytes have been shown to circulate in higher numbers in gonadectomized dogs compared to intact individuals [64], plausibly stimulating cell division through T-lymphocyte-bound LH receptor activation. These findings correspond to outcomes of in vitro experiments and thus, prospective case–control trials are required to further verify a causative association [63,65]. Nonetheless, if dunnarts with testicular degeneration have comparable endocrine profiles to gonadectomized dogs, it could potentially lead to similar pathophysiological effects of sustained supraphysiologic LH levels. It is also unknown, however, if the onset of testicular degeneration in the male dunnarts included in this study occurred earlier or later in life. We speculate testicular degeneration is likely to be a consequence of aging, as its occurrence in younger or pre-pubertal dunnarts would likely have led to other significant health issues due to the loss of negative feedback to the hypothalamus and anterior pituitary. In dogs and humans, sustained supraphysiologic levels of LH have been documented to lead to metabolic (hypothyroidism), musculoskeletal issues (increased ligament laxity) or urinary issues (incontinence) [63,65]. Lesions compatible with these health issues were not observed in the dunnarts included in this study.

The insufficient leukocytic marker immunolabeling observed in nodal control tissue hampered our ability to utilize IHC to definitively immunophenotype the cutaneous lymphomas observed. Possible issues that could have negatively impacted IHC immunolabeling quality include poor preservation of sample prior to freezing, age of samples (nearly a decade old), preservation artefact (freezing), lack of proper validation procedures in the species and prolonged or variable formalin fixation time between samples [66]. Pan-cytokeratin did provide adequate diagnostic immunolabeling in control tissue, facing the same preservation and fixation challenges. However, with pan-cytokeratin being a different epitope, it is unlikely to be equally negatively influenced by the aforementioned challenges that did affect leukocytic and hematopoietic markers.

Hair loss has been extensively documented in captive wildlife and can be caused by stress, excessive grooming, parasites, integument diseases, dietary deficiencies and infections (fungal and bacterial), amongst others. The alopecia observed could have some similarity with telogen effluvium, a well-described condition in domestic animals in which there is pelage loss associated with extrinsic physical, physiological or mental stressors or improper diets. With telogen effluvium, there is excessive generalized hair shedding with a reduction in hair volume that does not render complete baldness. The pattern of alopecia observed in the three males affected were similar to what has been reported in macaques [67] and bats [68], where stress has been detected as an important cause. Further, comedone formation, although infrequently observed, could support underlying stress or endocrinopathies as it has been documented in small animals [69].

Barbering—the act of plucking whiskers and fur in mice due to excessive grooming—would be an important differential diagnosis in these cases if it is confirmed that captive dunnarts can replicate such behavior responding to social dominance [70]. Excessive hair pulling (trichotillomania) is different and has also been observed in macaques and can be partially corrected with environmental enrichment [71]. Despite whiskers being intact in this study, males were over-represented with alopecia, which may grant the exploration of hierarchical conflicts with females as a cause, as female mice are more likely to apply this behavior than males. As chronic stress is a well-recognized issue in captive wildlife and has been found to be associated with conditions observed in this study, future attempts at establishing captive breeding dunnart colonies could benefit from exploring environmental enrichment as well as the incorporation of non-invasive fecal glucocorticoid metabolite testing or hair cortisol levels as biomarkers to monitor stress levels [72].

Senescence is a risk factor for cancer development. Both mature age and cancer share disease mechanisms such as genomic instability, epigenetic changes, altered nutrient sensing metabolism and are fundamentally divergent in others, such as telomere attrition in geriatric cells in contrast to the ability to perform rapid cell division and high energy consumption of neoplastic cells [73]. The lifespan of captive Julia Creek dunnarts was increased by approximately one year, and so, the molecular mechanisms associated with aging are suggested as the underlying mechanisms for the interconnectedness of aging and neoplasia observed in this study. Nonetheless, future studies should aim at investigating other potential etiologies including oncogenic viruses.

Reproductive aging is a crucial phenomenon of concern in any conservation breeding program, particularly for genetically valuable wildlife, as research has shown long-term captivity can lead to prolonged exposure to endogenous sex steroids and long non-reproductive intervals, resulting in asymmetric reproductive aging and low reproductive performance [74]. In asymmetric reproductive aging, females in which first breeding attempts and pregnancies are substantially delayed result in frequent cyclic fluctuations of estrogen, subsequently causing reproductive disease, as tested in cheetahs (Acinonyx jubatus) [75]. The theory is interesting and could be applied to dasyurids, although its veracity remains to be tested. To avoid asymmetric reproductive aging, reduced fertility and irreversible acyclicity, especially in short-lived wildlife, captive breeding programs can benefit from incorporating a basic cytological assessment of reproductive cycle phases (e.g., urogenital/vaginal mucus or urine smears [76,77,78,79,80] to monitor fertility and perform early and regular selective breeding of young and genetically valued females). Alternatively, captive breeding programs could benefit from the use of pioneered assisted reproductive technologies to induce pregnancies as early as possible [74,81,82], or monitoring for behavioral signals (e.g., wheel running activity [83]).

5. Conclusions

From the findings reported in this study, it can be inferred that captive breeding colonies of dunnarts can benefit from including health examinations into their program, particularly upon reaching geriatric age. This study is the first to document a comprehensive list of spontaneous reproductive and significant non-reproductive conditions affecting geriatric dunnarts to improve the paucity of the dasyurid disease literature. These results paired with effective management prescriptions, including captive breeding, maintenance and restoration of land with essential structure for the species, are vital for the long-term conservation success of Julia Creek dunnarts.