Supplementation with Rumen-Protected Methionine Reduced the Parasitic Effect of Haemonchus contortus in Goats
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals, Management and Experimental Design
2.2. Animal Samples and Measurements
2.3. Chemical Analyses and Analytical Procedures
2.4. Statistical Analysis
3. Results
3.1. Experimental Diets and Growth Performance
3.2. Parasitological and Physiological Parameters
3.3. Serological and Blood Analysis
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Diet Composition | Control | Low Methionine | High Methionine |
---|---|---|---|
Ingredient composition (g/kg DM) | |||
Maize | 221.5 | 225 | 230 |
Soybean Meal | 103 | 96 | 83 |
Hay (Digitaria spp.) | 675 | 675 | 675 |
Methionine (Smartamine®) | 0 | 3.5 | 11.5 |
Mineral Mixture | 0.5 | 0.5 | 0.5 |
Nutrient composition (g/Kg) | |||
Dry Matter | 931.4 | 931.6 | 920.7 |
Organic Matter | 921.9 | 921.6 | 920.7 |
Crude Protein | 75.2 | 74.9 | 75.4 |
Neutral Detergent Fiber | 537.2 | 536.0 | 533.7 |
Acid Detergent Fiber | 304.9 | 304.3 | 303.1 |
Acid Detergent Lignin | 53.9 | 53.9 | 53.8 |
Methionine | 1.02 | 3.31 | 8.56 |
Predictive nutritive value | |||
Metabolizable Energy (MJ/Kg) | 9.25 | 9.25 | 9.25 |
Net Energy (MJ/Kg) | 5.47 | 5.47 | 5.48 |
PDI 1 (g/Kg) | 85.66 | 85.65 | 85.67 |
PDIA 2 | 44.36 | 44.38 | 44.42 |
MetDi 3 (% PDI) | 1.87 | 2.02 | 2.75 |
LysDi 4 (% PDI) | 6.60 | 6.56 | 6.62 |
HisDi 5 (% PDI) | 2.12 | 2.12 | 2.12 |
Period 1 21 to 26 dpi 1 | Period 2 35 to 40 dpi | p-Value | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
C 2 | LM 3 | HM 4 | C | LM | HM | SEM | G 5 | P 6 | G × P | |
Intake (g/d) | ||||||||||
DMI 7 | 490.5 | 543.2 | 506.0 | 475.8 | 516.1 | 527.0 | 12.46 | 0.102 | 0.697 | 0.505 |
OMI 8 | 438.3 | 489.0 | 457.8 | 430.2 | 471.1 | 477.5 | 11.20 | 0.064 | 0.895 | 0.599 |
CPI 9 | 32.9 a | 35.3 a | 34.1 a | 36.4 b | 38.3 b | 44.5 b | 1.48 | 0.189 | 0.010 | 0.270 |
NDFI 10 | 238.4 | 269.6 | 228.4 | 243.4 | 268.6 | 266.0 | 9.08 | 0.186 | 0.281 | 0.415 |
ADFI 11 | 137.1 | 152.9 | 135.3 | 139.9 | 152.7 | 150.7 | 5.19 | 0.286 | 0.418 | 0.649 |
Total tract digestibility (g/kg DM) | ||||||||||
DDM 12 | 671.4 a | 697.1 a | 660.9 a | 642.3 b | 656.1 b | 656.0 b | 6.92 | 0.199 | 0.014 | 0.313 |
DOM 13 | 684.9 a | 711.6 a | 677.6 a | 661.5 b | 679.3 b | 673.4 b | 6.44 | 0.105 | 0.033 | 0.431 |
DCP 14 | 428.0 a | 477.9 a | 486.8 a | 545.5 b | 572.6 b | 638.7 b | 22.92 | 0.166 | 0.001 | 0.765 |
ADG 15 (g/d) | 27.3 | 35.2 | 36.7 | 27.3 | 35.2 | 36.7 | 4.38 | 0.417 | 1.000 | 1.000 |
C 1 | LM 2 | HM 3 | SEM 4 | p-Value | |||
---|---|---|---|---|---|---|---|
G 5 | dpi 6 | G × dpi | |||||
FEC (eggs/g of faeces) | 5369 | 2773 | 3001 | 1652 | 0.366 | <0.0001 | 0.577 |
Female | 389 | 291 | 290 | 94 | 0.727 | - | - |
Male | 349 | 297 | 202 | 161 | 0.653 | - | - |
Parasite burden | 739 | 589 | 492 | 292 | 0.702 | - | - |
C 1 | LM 2 | HM 3 | SEM 4 | p-Value | |||
---|---|---|---|---|---|---|---|
G 5 | dpi 6 | G × dpi | |||||
IgE anti-L3 7 | 0.175 a | 0.141 b | 0.157 ab | 0.009 | 0.017 | 0.009 | 0.789 |
IgE anti-ESP 8 | 0.143 | 0.131 | 0.108 | 0.005 | 0.130 | <0.0001 | 0.667 |
IgA anti-L3 | 0.183 a | 0.200 a | 0.225 b | 0.021 | 0.021 | 0.009 | 0.994 |
IgA anti-ESP | 0.159 a | 0.255 b | 0.243 b | 0.024 | 0.008 | 0.013 | 0.984 |
U_Tyrosine 9 | 1.624 a | 1.521 a | 1.346 b | 0.061 | 0.005 | <0.0001 | 0.952 |
Complete Blood Count (%) | C 1 | LM 2 | HM 3 | SEM 4 | p-Value | ||
---|---|---|---|---|---|---|---|
G 5 | dpi 6 | G × dpi | |||||
Lymphocytes | 40.04 | 40.37 | 39.63 | 1.3 | 0.828 | 0.003 | 0.828 |
Monocytes | 4.06 | 4.26 | 4.16 | 1.0 | 0.324 | 0.01 | 0.668 |
Polymorphonuclear neutrophils | 47.15 a | 45.08 a | 51.95 b | 1.6 | 0.004 | 0.012 | 0.674 |
Eosinophils | 8.61 a | 10.16 a | 4.17 b | 1.2 | 0.002 | 0.343 | 0.443 |
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Montout, L.; Bahloul, L.; Feuillet, D.; Jean-Bart, M.; Archimède, H.; Bambou, J.-C. Supplementation with Rumen-Protected Methionine Reduced the Parasitic Effect of Haemonchus contortus in Goats. Vet. Sci. 2023, 10, 559. https://doi.org/10.3390/vetsci10090559
Montout L, Bahloul L, Feuillet D, Jean-Bart M, Archimède H, Bambou J-C. Supplementation with Rumen-Protected Methionine Reduced the Parasitic Effect of Haemonchus contortus in Goats. Veterinary Sciences. 2023; 10(9):559. https://doi.org/10.3390/vetsci10090559
Chicago/Turabian StyleMontout, Laura, Lahlou Bahloul, Dalila Feuillet, Max Jean-Bart, Harry Archimède, and Jean-Christophe Bambou. 2023. "Supplementation with Rumen-Protected Methionine Reduced the Parasitic Effect of Haemonchus contortus in Goats" Veterinary Sciences 10, no. 9: 559. https://doi.org/10.3390/vetsci10090559