Effect of Mineral Element Imbalance on Neutrophil Respiratory Burst Function and Inflammatory and Antioxidant Responses in Sheep
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Experimental Design
2.2. Sample Collection
2.3. Routine Blood Tests
2.4. Blood Gas and Electrolyte Analysis
2.5. Flow Cytometry Analysis
2.6. ELISA, Colorimetric and Hydroxylamine Method Detection
2.7. Statistical Analysis
3. Results
3.1. Effect of Central Venous Catheters on Blood Routines in Sheep
3.2. Effect of EDTA Injection on Blood Gas and Electrolytes in Sheep
3.3. Mineral Element Homeostasis Imbalance Affects Neutrophil Respiratory Burst Function
3.4. Effects of Mineral Element Homeostatic Imbalance on Serum IL-6, IL-1β and TNF-α
3.5. Effects of Mineral Element Homeostatic Imbalance on Serum MMP7 and TIMP1
3.6. Effects of Mineral Element Homeostatic Imbalance on Serum CuZn-SOD, GH-PX, iNOS, TNOS, T-SOD and CP Vitality
3.7. Effects of Mineral Element Homeostatic Imbalance on Serum Blood Urea Nitrogen (BUN) and Creatinine (Crea)
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Item | Pre-Operation | Post-Operation |
---|---|---|
HCT/% | 33.0 ± 1.0 | 31.0 ± 3.0 |
HGB/(g/dL) | 10.73 ± 0.15 | 10.53 ± 1.12 |
MCHC/(g/dL) | 32.60 ± 0.53 | 33.80 ± 0.10 |
WBC/(109/L) | 9.57 ± 1.04 | 10.33 ± 1.10 |
GRANS/(109/L) | 5.70 ± 1.25 | 7.70 ± 1.78 |
L/M | 9.59 ± 0.04 | 9.41 ± 0.12 |
Item | Control Group | EDTA Group |
---|---|---|
pH | 7.40 ± 0.42 | 7.44 ± 0.45 |
PCO2 | 36.00 ± 4.27 | 35.88 ± 4.89 |
HCO3− | 21.32 ± 2.77 | 22.70 ± 3.54 |
tCO2 | 22.31 ± 2.88 | 23.80 ± 3.64 |
Na+ | 143.60 ± 1.90 | 137.13 ± 3.64 ** |
K+ | 3.83 ± 0.35 | 2.35 ± 0.43 ** |
Cl− | 108.10 ± 2.42 | 103.50 ± 2.62 ** |
Ca2+ | 1.07 ± 0.09 | 1.36 ± 0.36 |
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Liu, W.; Wang, D.; Zhou, Q.; Wang, J.; Lian, S. Effect of Mineral Element Imbalance on Neutrophil Respiratory Burst Function and Inflammatory and Antioxidant Responses in Sheep. Vet. Sci. 2023, 10, 241. https://doi.org/10.3390/vetsci10040241
Liu W, Wang D, Zhou Q, Wang J, Lian S. Effect of Mineral Element Imbalance on Neutrophil Respiratory Burst Function and Inflammatory and Antioxidant Responses in Sheep. Veterinary Sciences. 2023; 10(4):241. https://doi.org/10.3390/vetsci10040241
Chicago/Turabian StyleLiu, Weiqi, Di Wang, Qijun Zhou, Jianfa Wang, and Shuai Lian. 2023. "Effect of Mineral Element Imbalance on Neutrophil Respiratory Burst Function and Inflammatory and Antioxidant Responses in Sheep" Veterinary Sciences 10, no. 4: 241. https://doi.org/10.3390/vetsci10040241